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Chemokine-directed migration of tumor-inhibitory neural progenitor cells towards an intracranially growing glioma.

Honeth, Gabriella LU ; Staflin, Karin LU ; Kalliomäki, Suzanne LU ; Lindvall, Magnus LU and Kjellman, Christian LU (2006) In Experimental Cell Research 312(8). p.1265-1276
Abstract
We have earlier shown that the rat neural progenitor cell line HiB5 is capable of suppressing intracranial growth of glioma cells in Fisher rats. Unlike some neural progenitor cells, HiB5 cells have not shown homing capacity towards glioma cells growing intracranially. In this study, we have genetically modified HiB5 progenitor cells to over-express the chemokine receptor CXCR3. We show that the introduced receptor is functionally responding to ligand stimulation with increased phosphorylation levels of ERK and SAPK/JNK and a transcriptional response of an AP-1 reporter system introduced into HIB5 cells. These transfected progenitor cells migrate in vitro in response to IP-10 and I-TAC. Further, we show an enhanced in vivo migration of the... (More)
We have earlier shown that the rat neural progenitor cell line HiB5 is capable of suppressing intracranial growth of glioma cells in Fisher rats. Unlike some neural progenitor cells, HiB5 cells have not shown homing capacity towards glioma cells growing intracranially. In this study, we have genetically modified HiB5 progenitor cells to over-express the chemokine receptor CXCR3. We show that the introduced receptor is functionally responding to ligand stimulation with increased phosphorylation levels of ERK and SAPK/JNK and a transcriptional response of an AP-1 reporter system introduced into HIB5 cells. These transfected progenitor cells migrate in vitro in response to IP-10 and I-TAC. Further, we show an enhanced in vivo migration of the CXCR3 transfected HiB5 cells over the corpus callosum towards an IP-10 and I-TAC expressing glioma, as compared to wild type HiB5 cells. Our data indicate that it is possible to take advantage of chemokines natural capacity to initiate migratory responses, and to use this ability to enhance tumor-inhibitory neural progenitor cells to target an intracranially growing glioma. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Glioma, Neural progenitor cells, Chemokines, Migration, IP-10, I-TAC, corpus callosum, Rat
in
Experimental Cell Research
volume
312
issue
8
pages
1265 - 1276
publisher
Academic Press
external identifiers
  • wos:000236980400005
  • pmid:16434036
  • scopus:33646036119
ISSN
1090-2422
DOI
10.1016/j.yexcr.2005.12.018
language
English
LU publication?
yes
id
5d44a3b4-dde7-4f27-bf31-dbb8f8f2ef89 (old id 150135)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16434036&dopt=Abstract
date added to LUP
2007-07-13 13:53:34
date last changed
2019-02-20 04:31:55
@article{5d44a3b4-dde7-4f27-bf31-dbb8f8f2ef89,
  abstract     = {We have earlier shown that the rat neural progenitor cell line HiB5 is capable of suppressing intracranial growth of glioma cells in Fisher rats. Unlike some neural progenitor cells, HiB5 cells have not shown homing capacity towards glioma cells growing intracranially. In this study, we have genetically modified HiB5 progenitor cells to over-express the chemokine receptor CXCR3. We show that the introduced receptor is functionally responding to ligand stimulation with increased phosphorylation levels of ERK and SAPK/JNK and a transcriptional response of an AP-1 reporter system introduced into HIB5 cells. These transfected progenitor cells migrate in vitro in response to IP-10 and I-TAC. Further, we show an enhanced in vivo migration of the CXCR3 transfected HiB5 cells over the corpus callosum towards an IP-10 and I-TAC expressing glioma, as compared to wild type HiB5 cells. Our data indicate that it is possible to take advantage of chemokines natural capacity to initiate migratory responses, and to use this ability to enhance tumor-inhibitory neural progenitor cells to target an intracranially growing glioma.},
  author       = {Honeth, Gabriella and Staflin, Karin and Kalliomäki, Suzanne and Lindvall, Magnus and Kjellman, Christian},
  issn         = {1090-2422},
  keyword      = {Glioma,Neural progenitor cells,Chemokines,Migration,IP-10,I-TAC,corpus callosum,Rat},
  language     = {eng},
  number       = {8},
  pages        = {1265--1276},
  publisher    = {Academic Press},
  series       = {Experimental Cell Research},
  title        = {Chemokine-directed migration of tumor-inhibitory neural progenitor cells towards an intracranially growing glioma.},
  url          = {http://dx.doi.org/10.1016/j.yexcr.2005.12.018},
  volume       = {312},
  year         = {2006},
}