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Modeling Metastatic Colonization in a Decellularized Organ Scaffold-Based Perfusion Bioreactor

Rafaeva, Maria ; Horton, Edward R. ; Jensen, Adina R.D. ; Madsen, Chris D. LU ; Reuten, Raphael ; Willacy, Oliver ; Brøchner, Christian B. ; Jensen, Thomas H. ; Zornhagen, Kamilla Westarp and Crespo, Marina , et al. (2022) In Advanced healthcare materials 11(1).
Abstract

Metastatic cancer spread is responsible for most cancer-related deaths. To colonize a new organ, invading cells adapt to, and remodel, the local extracellular matrix (ECM), a network of proteins and proteoglycans underpinning all tissues, and a critical regulator of homeostasis and disease. However, there is a major lack in tools to study cancer cell behavior within native 3D ECM. Here, an in-house designed bioreactor, where mouse organ ECM scaffolds are perfused and populated with cells that are challenged to colonize it, is presented. Using a specialized bioreactor chamber, it is possible to monitor cell behavior microscopically (e.g., proliferation, migration) within the organ scaffold. Cancer cells in this system recapitulate cell... (More)

Metastatic cancer spread is responsible for most cancer-related deaths. To colonize a new organ, invading cells adapt to, and remodel, the local extracellular matrix (ECM), a network of proteins and proteoglycans underpinning all tissues, and a critical regulator of homeostasis and disease. However, there is a major lack in tools to study cancer cell behavior within native 3D ECM. Here, an in-house designed bioreactor, where mouse organ ECM scaffolds are perfused and populated with cells that are challenged to colonize it, is presented. Using a specialized bioreactor chamber, it is possible to monitor cell behavior microscopically (e.g., proliferation, migration) within the organ scaffold. Cancer cells in this system recapitulate cell signaling observed in vivo and remodel complex native ECM. Moreover, the bioreactors are compatible with co-culturing cell types of different genetic origin comprising the normal and tumor microenvironment. This degree of experimental flexibility in an organ-specific and 3D context, opens new possibilities to study cell–cell and cell–ECM interplay and to model diseases in a controllable organ-specific system ex vivo.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cancer metastasis, experimental methods, extracellular matrix, specialized bioreactors
in
Advanced healthcare materials
volume
11
issue
1
article number
2100684
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:34734500
  • scopus:85119064261
ISSN
2192-2640
DOI
10.1002/adhm.202100684
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2021 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH
id
5d4d2e0c-3ce4-4b87-a754-71eb50c1a1c1
date added to LUP
2021-12-03 15:16:51
date last changed
2025-03-09 23:32:40
@article{5d4d2e0c-3ce4-4b87-a754-71eb50c1a1c1,
  abstract     = {{<p>Metastatic cancer spread is responsible for most cancer-related deaths. To colonize a new organ, invading cells adapt to, and remodel, the local extracellular matrix (ECM), a network of proteins and proteoglycans underpinning all tissues, and a critical regulator of homeostasis and disease. However, there is a major lack in tools to study cancer cell behavior within native 3D ECM. Here, an in-house designed bioreactor, where mouse organ ECM scaffolds are perfused and populated with cells that are challenged to colonize it, is presented. Using a specialized bioreactor chamber, it is possible to monitor cell behavior microscopically (e.g., proliferation, migration) within the organ scaffold. Cancer cells in this system recapitulate cell signaling observed in vivo and remodel complex native ECM. Moreover, the bioreactors are compatible with co-culturing cell types of different genetic origin comprising the normal and tumor microenvironment. This degree of experimental flexibility in an organ-specific and 3D context, opens new possibilities to study cell–cell and cell–ECM interplay and to model diseases in a controllable organ-specific system ex vivo.</p>}},
  author       = {{Rafaeva, Maria and Horton, Edward R. and Jensen, Adina R.D. and Madsen, Chris D. and Reuten, Raphael and Willacy, Oliver and Brøchner, Christian B. and Jensen, Thomas H. and Zornhagen, Kamilla Westarp and Crespo, Marina and Grønseth, Dina S. and Nielsen, Sebastian R. and Idorn, Manja and Straten, Per thor and Rohrberg, Kristoffer and Spanggaard, Iben and Højgaard, Martin and Lassen, Ulrik and Erler, Janine T. and Mayorca-Guiliani, Alejandro E.}},
  issn         = {{2192-2640}},
  keywords     = {{cancer metastasis; experimental methods; extracellular matrix; specialized bioreactors}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Advanced healthcare materials}},
  title        = {{Modeling Metastatic Colonization in a Decellularized Organ Scaffold-Based Perfusion Bioreactor}},
  url          = {{http://dx.doi.org/10.1002/adhm.202100684}},
  doi          = {{10.1002/adhm.202100684}},
  volume       = {{11}},
  year         = {{2022}},
}