Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates.
(2015) In Nanoscale 7(43). p.18020-18024- Abstract
- The fluorescence interference contrast (FLIC) effect prevents the use of fluorescence techniques to probe the continuity and fluidity of supported lipid bilayers on reflective materials due to a lack of detectable fluorescence. Here we show that adding nanostructures onto reflective surfaces to locally confer a certain distance between the deposited fluorophores and the reflecting surface enables fluorescence detection on the nanostuctures. The nanostructures consist of either deposited nanoparticles or epitaxial nanowires directly grown on the substrate and are designed such that they can support a lipid bilayer. This simple method increases the fluorescence signal sufficiently to enable bilayer fluorescence detection and to observe the... (More)
- The fluorescence interference contrast (FLIC) effect prevents the use of fluorescence techniques to probe the continuity and fluidity of supported lipid bilayers on reflective materials due to a lack of detectable fluorescence. Here we show that adding nanostructures onto reflective surfaces to locally confer a certain distance between the deposited fluorophores and the reflecting surface enables fluorescence detection on the nanostuctures. The nanostructures consist of either deposited nanoparticles or epitaxial nanowires directly grown on the substrate and are designed such that they can support a lipid bilayer. This simple method increases the fluorescence signal sufficiently to enable bilayer fluorescence detection and to observe the recovery of fluorescence after photobleaching in order to assess lipid bilayer formation on any reflective surface. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8148834
- author
- Dabkowska, Aleksandra LU ; Piret, Gaelle LU ; Niman, Cassandra LU ; Lard, Mercy LU ; Linke, Heiner LU ; Nylander, Tommy LU and Prinz, Christelle LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nanoscale
- volume
- 7
- issue
- 43
- pages
- 18020 - 18024
- publisher
- Royal Society of Chemistry
- external identifiers
-
- pmid:26482860
- wos:000364048900005
- scopus:84946108249
- pmid:26482860
- ISSN
- 2040-3372
- DOI
- 10.1039/c5nr05427c
- language
- English
- LU publication?
- yes
- id
- 5d937d2f-e859-4ea4-b093-839c5ae176d1 (old id 8148834)
- date added to LUP
- 2016-04-01 09:59:24
- date last changed
- 2023-10-11 14:59:44
@article{5d937d2f-e859-4ea4-b093-839c5ae176d1, abstract = {{The fluorescence interference contrast (FLIC) effect prevents the use of fluorescence techniques to probe the continuity and fluidity of supported lipid bilayers on reflective materials due to a lack of detectable fluorescence. Here we show that adding nanostructures onto reflective surfaces to locally confer a certain distance between the deposited fluorophores and the reflecting surface enables fluorescence detection on the nanostuctures. The nanostructures consist of either deposited nanoparticles or epitaxial nanowires directly grown on the substrate and are designed such that they can support a lipid bilayer. This simple method increases the fluorescence signal sufficiently to enable bilayer fluorescence detection and to observe the recovery of fluorescence after photobleaching in order to assess lipid bilayer formation on any reflective surface.}}, author = {{Dabkowska, Aleksandra and Piret, Gaelle and Niman, Cassandra and Lard, Mercy and Linke, Heiner and Nylander, Tommy and Prinz, Christelle}}, issn = {{2040-3372}}, language = {{eng}}, number = {{43}}, pages = {{18020--18024}}, publisher = {{Royal Society of Chemistry}}, series = {{Nanoscale}}, title = {{Surface nanostructures for fluorescence probing of supported lipid bilayers on reflective substrates.}}, url = {{https://lup.lub.lu.se/search/files/1455179/8866638}}, doi = {{10.1039/c5nr05427c}}, volume = {{7}}, year = {{2015}}, }