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Steroid hydroxylation using immobilized spores of Curvularia lunata germinated in situ

Ohlson, Sten ; Flygare, Susanne ; Larsson, Per-Olof LU and Mosbach, Klaus LU (1980) In European Journal of Applied Microbiology and Biotechnology 10. p.1-9
Abstract
Spores of Curvularia lunata were immobilized in polyacrylamide granules and in calcium alginate beads (2–3 mm in diam.). Germination of the spores, initiated by the addition of nutrients, resulted in an even distribution of mycelium throughout the beads after 48 h. Such beads were used for the conversion of cortexolone to cortisol by steroid-11β-hydroxylation. In order to improve the steroid transforming ability several parameters were studied. It was found that preparations based on calcium alginate gave the best results.

The possible merits of immobilizing spores rather than vegetative cells, followed by in situ germination are discussed also for other microorganisms and immobilization processes.
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Immobilized spores, Curvularia lunata, In situ germination
in
European Journal of Applied Microbiology and Biotechnology
volume
10
pages
9 pages
publisher
Springer
external identifiers
  • scopus:0002547527
ISSN
0171-1741
DOI
10.1007/BF00504722
language
English
LU publication?
yes
id
5d97e991-51f2-4b91-8f98-a2337bee566f
date added to LUP
2024-06-19 09:44:29
date last changed
2024-09-09 10:04:44
@article{5d97e991-51f2-4b91-8f98-a2337bee566f,
  abstract     = {{Spores of <i>Curvularia lunata</i> were immobilized in polyacrylamide granules and in calcium alginate beads (2–3 mm in diam.). Germination of the spores, initiated by the addition of nutrients, resulted in an even distribution of mycelium throughout the beads after 48 h. Such beads were used for the conversion of cortexolone to cortisol by steroid-11<i>β</i>-hydroxylation. In order to improve the steroid transforming ability several parameters were studied. It was found that preparations based on calcium alginate gave the best results.<br/><br/>The possible merits of immobilizing spores rather than vegetative cells, followed by in situ germination are discussed also for other microorganisms and immobilization processes.}},
  author       = {{Ohlson, Sten and Flygare, Susanne and Larsson, Per-Olof and Mosbach, Klaus}},
  issn         = {{0171-1741}},
  keywords     = {{Immobilized spores; Curvularia lunata; In situ germination}},
  language     = {{eng}},
  pages        = {{1--9}},
  publisher    = {{Springer}},
  series       = {{European Journal of Applied Microbiology and Biotechnology}},
  title        = {{Steroid hydroxylation using immobilized spores of <i>Curvularia lunata</i> germinated in situ}},
  url          = {{http://dx.doi.org/10.1007/BF00504722}},
  doi          = {{10.1007/BF00504722}},
  volume       = {{10}},
  year         = {{1980}},
}