A novel nonneuronal catecholaminergic system : Exocrine pancreas synthesizes and releases dopamine
Mezey, Éva LU ; Eisenhofer, Graeme ; Harta, Gyöngyi ; Hansson, Stefan LU
; Gould, Lydia
; Hunyady, Béla
and Hoffman, Beth J.
(1996)
In Proceedings of the National Academy of Sciences of the United States of America
93(19).
p.10377-10382
- Abstract
Cells of the exocrine pancreas produce digestive enzymes potentially harmful to the intestinal mucosa. Dopamine has been reported to protect against mucosal injury. In looking for the source of dopamine in the small intestine, we found that the duodenal juice contains high levels of dopamine and that the pancreas itself has a high dopamine [and dihydroxyphenylalanine (dopa)] content that does not change significantly after chemical sympathectomy. Furthermore, we were able to demonstrate tyrosine hydroxylase (TH) activity in control pancreas as well as in pancreas from rats after chemical sympathectomy. Immunostaining and in situ hybridization histochemistry confirmed both the presence of TH, dopamine, and the dopamine transporter, and... (More)
Cells of the exocrine pancreas produce digestive enzymes potentially harmful to the intestinal mucosa. Dopamine has been reported to protect against mucosal injury. In looking for the source of dopamine in the small intestine, we found that the duodenal juice contains high levels of dopamine and that the pancreas itself has a high dopamine [and dihydroxyphenylalanine (dopa)] content that does not change significantly after chemical sympathectomy. Furthermore, we were able to demonstrate tyrosine hydroxylase (TH) activity in control pancreas as well as in pancreas from rats after chemical sympathectomy. Immunostaining and in situ hybridization histochemistry confirmed both the presence of TH, dopamine, and the dopamine transporter, and the mRNAs encoding TH and dopamine transporter, and the presence of both types of vesicular monoamine transporters in the exocrine cells of the pancreas. Since there are no catecholaminergic enteric ganglia in the pancreas, the above results indicate that pancreatic cells have all the characteristics of dopamine-producing cells. We suggest that the pancreas is an important source of nonneuronal dopamine in the body, and that this dopamine has a role in protecting the intestinal mucosa and suggests that dopamine D1b receptor agonists might be used to help mucosal healing in the gastrointestinal tract.
(Less)
- author
- Mezey, Éva
LU
; Eisenhofer, Graeme
; Harta, Gyöngyi
; Hansson, Stefan
LU
; Gould, Lydia
; Hunyady, Béla
and Hoffman, Beth J.
- publishing date
- 1996-09-17
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- dopamine receptor, monoamine transporters, mucosal healing
- in
- Proceedings of the National Academy of Sciences of the United States of America
- volume
- 93
- issue
- 19
- pages
- 10377 - 10382
- publisher
- National Academy of Sciences
- external identifiers
-
- scopus:0029813426
- pmid:8816808
- ISSN
- 0027-8424
- DOI
- 10.1073/pnas.93.19.10377
- language
- English
- LU publication?
- no
- id
- 5daacecc-93e6-44f4-bff1-62ef896d2641
- date added to LUP
- 2021-02-12 10:47:21
- date last changed
- 2024-04-04 01:34:26
@article{5daacecc-93e6-44f4-bff1-62ef896d2641,
abstract = {{<p>Cells of the exocrine pancreas produce digestive enzymes potentially harmful to the intestinal mucosa. Dopamine has been reported to protect against mucosal injury. In looking for the source of dopamine in the small intestine, we found that the duodenal juice contains high levels of dopamine and that the pancreas itself has a high dopamine [and dihydroxyphenylalanine (dopa)] content that does not change significantly after chemical sympathectomy. Furthermore, we were able to demonstrate tyrosine hydroxylase (TH) activity in control pancreas as well as in pancreas from rats after chemical sympathectomy. Immunostaining and in situ hybridization histochemistry confirmed both the presence of TH, dopamine, and the dopamine transporter, and the mRNAs encoding TH and dopamine transporter, and the presence of both types of vesicular monoamine transporters in the exocrine cells of the pancreas. Since there are no catecholaminergic enteric ganglia in the pancreas, the above results indicate that pancreatic cells have all the characteristics of dopamine-producing cells. We suggest that the pancreas is an important source of nonneuronal dopamine in the body, and that this dopamine has a role in protecting the intestinal mucosa and suggests that dopamine D1b receptor agonists might be used to help mucosal healing in the gastrointestinal tract.</p>}},
author = {{Mezey, Éva and Eisenhofer, Graeme and Harta, Gyöngyi and Hansson, Stefan and Gould, Lydia and Hunyady, Béla and Hoffman, Beth J.}},
issn = {{0027-8424}},
keywords = {{dopamine receptor; monoamine transporters; mucosal healing}},
language = {{eng}},
month = {{09}},
number = {{19}},
pages = {{10377--10382}},
publisher = {{National Academy of Sciences}},
series = {{Proceedings of the National Academy of Sciences of the United States of America}},
title = {{A novel nonneuronal catecholaminergic system : Exocrine pancreas synthesizes and releases dopamine}},
url = {{http://dx.doi.org/10.1073/pnas.93.19.10377}},
doi = {{10.1073/pnas.93.19.10377}},
volume = {{93}},
year = {{1996}},
}