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Adsorption of human carbonic anhydrase II onto silicon oxides surfaces : The effects of truncation in the N-terminal region

Billsten, P; Wahlgren, M LU and Elwing, H (1998) In The Colloid Science of Lipids New Paradigms for Self-Assembly in Science and Technology 108. p.161-165
Abstract (Swedish)
The adsorption of human carbonic anhydrase II pseudo-wild type (HCAIIpwt) and an N-terminally truncated version thereof onto silica surfaces were studied. The amount adsorbed and the adsorption kinetics were measured using in situ ellipsometry. A substantial difference was seen between the two proteins. The adsorbed amount of the truncated version (2.53 mg/m2) indicates an end-on orientation, while the HCAIIpwt seems to adsorb side-on (1.84 mg/m2). It is suggested that the orientation effects arise from the truncation. The truncation is known to unfold the two most N-terminal helical segments, which could inhibit adsorption with the N-terminal region facing the surface, due to steric repulsion.
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Protein adsorption, Ellipsometry , Carbonic Anhydrases, Mutant proteins, Solid Surfaces
in
The Colloid Science of Lipids New Paradigms for Self-Assembly in Science and Technology
editor
Lindman, B.; Ninham, B. W. ; and
volume
108
pages
5 pages
publisher
Springer
external identifiers
  • scopus:0542369594
ISSN
1435-1536
0340-255X
ISBN
978-3-7985-1112-5
978-3-7985-1655-7
DOI
10.1007/BFb0117973
language
English
LU publication?
yes
id
5e5b67bd-60b9-42a5-89e8-69d258e164cf
date added to LUP
2016-04-15 19:29:39
date last changed
2017-06-22 11:07:42
@inproceedings{5e5b67bd-60b9-42a5-89e8-69d258e164cf,
  abstract     = {The adsorption of human carbonic anhydrase II pseudo-wild type (HCAIIpwt) and an N-terminally truncated version thereof onto silica surfaces were studied. The amount adsorbed and the adsorption kinetics were measured using in situ ellipsometry. A substantial difference was seen between the two proteins. The adsorbed amount of the truncated version (2.53 mg/m2) indicates an end-on orientation, while the HCAIIpwt seems to adsorb side-on (1.84 mg/m2). It is suggested that the orientation effects arise from the truncation. The truncation is known to unfold the two most N-terminal helical segments, which could inhibit adsorption with the N-terminal region facing the surface, due to steric repulsion.},
  author       = {Billsten, P and Wahlgren, M and Elwing, H},
  booktitle    = {The Colloid Science of Lipids New Paradigms for Self-Assembly in Science and Technology},
  editor       = {Lindman, B. and Ninham, B. W. },
  isbn         = {978-3-7985-1112-5},
  issn         = {1435-1536},
  keyword      = {Protein adsorption,Ellipsometry ,Carbonic Anhydrases,Mutant proteins,Solid Surfaces},
  language     = {eng},
  pages        = {161--165},
  publisher    = {Springer},
  title        = {Adsorption of human carbonic anhydrase II onto silicon oxides surfaces : The effects of truncation in the N-terminal region},
  url          = {http://dx.doi.org/10.1007/BFb0117973},
  volume       = {108},
  year         = {1998},
}