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Adsorption of human carbonic anhydrase II onto silicon oxides surfaces : The effects of truncation in the N-terminal region

Billsten, P ; Wahlgren, M LU orcid and Elwing, H (1998) In Progress in Colloid & Polymer Science book series, PROGCOLLOID 108. p.161-165
Abstract
The adsorption of human carbonic anhydrase II pseudo-wild type (HCAIIpwt) and an N-terminally truncated version thereof onto silica surfaces were studied. The amount adsorbed and the adsorption kinetics were measured using in situ ellipsometry. A substantial difference was seen between the two proteins. The adsorbed amount of the truncated version (2.53 mg/m2) indicates an end-on orientation, while the HCAIIpwt seems to adsorb side-on (1.84 mg/m2). It is suggested that the orientation effects arise from the truncation. The truncation is known to unfold the two most N-terminal helical segments, which could inhibit adsorption with the N-terminal region facing the surface, due to steric repulsion.
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Protein adsorption, Ellipsometry, Carbonic Anhydrases, Mutant proteins, Solid Surfaces
host publication
The Colloid Science of Lipids New Paradigms for Self-Assembly in Science and Technology
series title
Progress in Colloid & Polymer Science book series, PROGCOLLOID
editor
Lindman, B. and Ninham, B. W.
volume
108
pages
5 pages
publisher
Springer
external identifiers
  • scopus:0542369594
ISSN
1435-1536
0340-255X
ISBN
978-3-7985-1112-5
978-3-7985-1655-7
DOI
10.1007/BFb0117973
language
English
LU publication?
yes
id
5e5b67bd-60b9-42a5-89e8-69d258e164cf
date added to LUP
2016-04-15 19:29:39
date last changed
2024-01-03 23:50:30
@inproceedings{5e5b67bd-60b9-42a5-89e8-69d258e164cf,
  abstract     = {{The adsorption of human carbonic anhydrase II pseudo-wild type (HCAIIpwt) and an N-terminally truncated version thereof onto silica surfaces were studied. The amount adsorbed and the adsorption kinetics were measured using in situ ellipsometry. A substantial difference was seen between the two proteins. The adsorbed amount of the truncated version (2.53 mg/m2) indicates an end-on orientation, while the HCAIIpwt seems to adsorb side-on (1.84 mg/m2). It is suggested that the orientation effects arise from the truncation. The truncation is known to unfold the two most N-terminal helical segments, which could inhibit adsorption with the N-terminal region facing the surface, due to steric repulsion.}},
  author       = {{Billsten, P and Wahlgren, M and Elwing, H}},
  booktitle    = {{The Colloid Science of Lipids New Paradigms for Self-Assembly in Science and Technology}},
  editor       = {{Lindman, B. and Ninham, B. W.}},
  isbn         = {{978-3-7985-1112-5}},
  issn         = {{1435-1536}},
  keywords     = {{Protein adsorption; Ellipsometry; Carbonic Anhydrases; Mutant proteins; Solid Surfaces}},
  language     = {{eng}},
  pages        = {{161--165}},
  publisher    = {{Springer}},
  series       = {{Progress in Colloid & Polymer Science book series, PROGCOLLOID}},
  title        = {{Adsorption of human carbonic anhydrase II onto silicon oxides surfaces : The effects of truncation in the N-terminal region}},
  url          = {{http://dx.doi.org/10.1007/BFb0117973}},
  doi          = {{10.1007/BFb0117973}},
  volume       = {{108}},
  year         = {{1998}},
}