Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Lifestyle chemical carcinogens associated with mutations in cell cycle regulatory genes increases the susceptibility to gastric cancer risk

Yadav, Ravi Prakash ; Ghatak, Souvik LU ; Chakraborty, Payel LU ; Lalrohlui, Freda ; Ravi, Kannan ; Kumar, Rajeev ; Pautu, Jeremy L ; Zomingthanga, John ; Chenkual, Saia and Muthukumaran, Rajendra , et al. (2018) In Environmental Science and Pollution Research 25. p.31691-31704
Abstract
In the present study, we correlated the various lifestyle habits and their associated mutations in cell cycle (P21 and MDM2) and DNA damage repair (MLH1) genes to investigate their role in gastric cancer (GC). Multifactor dimensionality reduction (MDR) analysis revealed the two-factor model of oral snuff and smoked meat as the significant model for GC risk. The interaction analysis between identified mutations and the significant demographic factors predicted that oral snuff is significantly associated with P21 3′UTR mutations. A total of five mutations in P21 gene, including three novel mutations in intron 2 (36651738G > A, 36651804A > T, 36651825G > T), were identified. In MLH1 gene, two variants were identified viz. one in exon... (More)
In the present study, we correlated the various lifestyle habits and their associated mutations in cell cycle (P21 and MDM2) and DNA damage repair (MLH1) genes to investigate their role in gastric cancer (GC). Multifactor dimensionality reduction (MDR) analysis revealed the two-factor model of oral snuff and smoked meat as the significant model for GC risk. The interaction analysis between identified mutations and the significant demographic factors predicted that oral snuff is significantly associated with P21 3′UTR mutations. A total of five mutations in P21 gene, including three novel mutations in intron 2 (36651738G > A, 36651804A > T, 36651825G > T), were identified. In MLH1 gene, two variants were identified viz. one in exon 8 (37053568A > G; 219I > V) and a novel 37088831C > G in intron 16. Flow cytometric analysis predicted DNA aneuploidy in 07 (17.5%) and diploidy in 33 (82.5%) tumor samples. The G2/M phase was significantly arrested in aneuploid gastric tumor samples whereas high S-phase fraction was observed in all the gastric tumor samples. This study demonstrated that environmental chemical carcinogens along with alteration in cell cycle regulatory (P21) and mismatch repair (MLH1) genes may be stimulating the susceptibility of GC by altering the DNA content level abnormally in tumors in the Mizo ethic population. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; and (Less)
publishing date
type
Contribution to journal
publication status
published
in
Environmental Science and Pollution Research
volume
25
pages
31691 - 31704
publisher
Springer
external identifiers
  • scopus:85053537688
ISSN
1614-7499
DOI
10.1007/s11356-018-3080-1
language
English
LU publication?
no
id
5eab2304-1f0c-4f03-9122-12c9af2664ac
date added to LUP
2021-11-09 15:58:00
date last changed
2022-03-26 23:01:56
@article{5eab2304-1f0c-4f03-9122-12c9af2664ac,
  abstract     = {{In the present study, we correlated the various lifestyle habits and their associated mutations in cell cycle (P21 and MDM2) and DNA damage repair (MLH1) genes to investigate their role in gastric cancer (GC). Multifactor dimensionality reduction (MDR) analysis revealed the two-factor model of oral snuff and smoked meat as the significant model for GC risk. The interaction analysis between identified mutations and the significant demographic factors predicted that oral snuff is significantly associated with P21 3′UTR mutations. A total of five mutations in P21 gene, including three novel mutations in intron 2 (36651738G > A, 36651804A > T, 36651825G > T), were identified. In MLH1 gene, two variants were identified viz. one in exon 8 (37053568A > G; 219I > V) and a novel 37088831C > G in intron 16. Flow cytometric analysis predicted DNA aneuploidy in 07 (17.5%) and diploidy in 33 (82.5%) tumor samples. The G2/M phase was significantly arrested in aneuploid gastric tumor samples whereas high S-phase fraction was observed in all the gastric tumor samples. This study demonstrated that environmental chemical carcinogens along with alteration in cell cycle regulatory (P21) and mismatch repair (MLH1) genes may be stimulating the susceptibility of GC by altering the DNA content level abnormally in tumors in the Mizo ethic population.}},
  author       = {{Yadav, Ravi Prakash and Ghatak, Souvik and Chakraborty, Payel and Lalrohlui, Freda and Ravi, Kannan and Kumar, Rajeev and Pautu, Jeremy L and Zomingthanga, John and Chenkual, Saia and Muthukumaran, Rajendra and Senthil Kumar, Nachimuthu}},
  issn         = {{1614-7499}},
  language     = {{eng}},
  pages        = {{31691--31704}},
  publisher    = {{Springer}},
  series       = {{Environmental Science and Pollution Research}},
  title        = {{Lifestyle chemical carcinogens associated with mutations in cell cycle regulatory genes increases the susceptibility to gastric cancer risk}},
  url          = {{http://dx.doi.org/10.1007/s11356-018-3080-1}},
  doi          = {{10.1007/s11356-018-3080-1}},
  volume       = {{25}},
  year         = {{2018}},
}