Absorption of kininogen from human plasma by Streptococcus pyogenes is followed by the release of bradykinin
(1997) In Biochemical Journal 326(3). p.657-660- Abstract
H-kininogen (high-molecular-mass kininogen, HK) is the precursor of the vasoactive peptide hormone bradykinin (BK). Previous work has demonstrated that HK binds to Streptococcus pyogenes through M-proteins, fibrous surface proteins and important virulence factors of these bacteria. Here we find that M-protein-expressing bacteria absorb HK from human plasma. The HK bound to the bacteria was found to be cleaved, and analysis of the degradation pattern suggested that the cleavage of HK at the bacterial surface is associated with the release of BK. Moreover, addition of activated plasma prekallikrein to bacteria preincubated with human plasma, resulted in BK release. This mechanism, by which a potent vasoactive and proinflammatory peptide... (More)
H-kininogen (high-molecular-mass kininogen, HK) is the precursor of the vasoactive peptide hormone bradykinin (BK). Previous work has demonstrated that HK binds to Streptococcus pyogenes through M-proteins, fibrous surface proteins and important virulence factors of these bacteria. Here we find that M-protein-expressing bacteria absorb HK from human plasma. The HK bound to the bacteria was found to be cleaved, and analysis of the degradation pattern suggested that the cleavage of HK at the bacterial surface is associated with the release of BK. Moreover, addition of activated plasma prekallikrein to bacteria preincubated with human plasma, resulted in BK release. This mechanism, by which a potent vasoactive and proinflammatory peptide is generated at the site of infection, should influence the host-parasite relationship during S. pyogenes infections.
(Less)
- author
- Ben Nasr, Abdelhakim
; Herwald, Heiko
LU
; Sjöbring, Ulf LU ; Renné, Thomas ; Müller-Esterl, Werner and Björck, Lars LU
- organization
- publishing date
- 1997-09-15
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemical Journal
- volume
- 326
- issue
- 3
- pages
- 657 - 660
- publisher
- Portland Press
- external identifiers
-
- pmid:9307013
- scopus:0030802005
- ISSN
- 0264-6021
- DOI
- 10.1042/bj3260657
- language
- English
- LU publication?
- yes
- id
- 5ef53a61-f222-482b-83cf-ef29696dce17
- date added to LUP
- 2019-05-27 06:39:38
- date last changed
- 2025-01-09 11:58:28
@article{5ef53a61-f222-482b-83cf-ef29696dce17, abstract = {{<p>H-kininogen (high-molecular-mass kininogen, HK) is the precursor of the vasoactive peptide hormone bradykinin (BK). Previous work has demonstrated that HK binds to Streptococcus pyogenes through M-proteins, fibrous surface proteins and important virulence factors of these bacteria. Here we find that M-protein-expressing bacteria absorb HK from human plasma. The HK bound to the bacteria was found to be cleaved, and analysis of the degradation pattern suggested that the cleavage of HK at the bacterial surface is associated with the release of BK. Moreover, addition of activated plasma prekallikrein to bacteria preincubated with human plasma, resulted in BK release. This mechanism, by which a potent vasoactive and proinflammatory peptide is generated at the site of infection, should influence the host-parasite relationship during S. pyogenes infections.</p>}}, author = {{Ben Nasr, Abdelhakim and Herwald, Heiko and Sjöbring, Ulf and Renné, Thomas and Müller-Esterl, Werner and Björck, Lars}}, issn = {{0264-6021}}, language = {{eng}}, month = {{09}}, number = {{3}}, pages = {{657--660}}, publisher = {{Portland Press}}, series = {{Biochemical Journal}}, title = {{Absorption of kininogen from human plasma by Streptococcus pyogenes is followed by the release of bradykinin}}, url = {{http://dx.doi.org/10.1042/bj3260657}}, doi = {{10.1042/bj3260657}}, volume = {{326}}, year = {{1997}}, }