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MreB of Streptomyces coelicolor is not required for vegetative growth but is involved in spore wall synthesis

Mazza, Paola ; Noens, Elke E. ; Schirner, Kathrin ; Grantcharova, Nina ; Mommass, A. Mieke ; Koerten, Henk K. ; Muth, Gunther ; Flärdh, Klas LU ; van Wezel, Gilles and Wohlleben, Wolfgang (2006) In Molecular Microbiology 60(4). p.838-852
Abstract
MreB forms a cytoskeleton in many rod-shaped bacteria which is involved in cell shape determination and chromosome segregation. PCR-based and Southern analysis of various actinomycetes, supported by analysis of genome sequences, revealed mreB homologues only in genera that form an aerial mycelium and sporulate. We analysed MreB in one such organism, Streptomyces coelicolor. Ectopic overexpression of mreB impaired growth, and caused swellings and lysis of hyphae. A null mutant with apparently normal vegetative growth was generated. However, aerial hyphae of this mutant were swelling and lysing; spores doubled their volume and lost their characteristic resistance to stress conditions. Loss of cell wall consistency was observed in... (More)
MreB forms a cytoskeleton in many rod-shaped bacteria which is involved in cell shape determination and chromosome segregation. PCR-based and Southern analysis of various actinomycetes, supported by analysis of genome sequences, revealed mreB homologues only in genera that form an aerial mycelium and sporulate. We analysed MreB in one such organism, Streptomyces coelicolor. Ectopic overexpression of mreB impaired growth, and caused swellings and lysis of hyphae. A null mutant with apparently normal vegetative growth was generated. However, aerial hyphae of this mutant were swelling and lysing; spores doubled their volume and lost their characteristic resistance to stress conditions. Loss of cell wall consistency was observed in MreB-depleted spores by transmission electron microscopy. An MreB–EGFP fusion was constructed to localize MreB in the mycelium. No clearly localized signal was seen in vegetative mycelium. However, strong fluorescence was observed at the septa of sporulating aerial hyphae, then as bipolar foci in young spores, and finally in a ring- or shell-like pattern inside the spores. Immunogold electron microscopy using MreB-specific antibodies revealed that MreB is located immediately underneath the internal spore wall. Thus, MreB is not essential for vegetative growth of S. coelicolor, but exerts its function in the formation of environmentally stable spores, and appears to primarily influence the assembly of the spore cell wall. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Microbiology
volume
60
issue
4
pages
838 - 852
publisher
Wiley-Blackwell
external identifiers
  • scopus:33646412602
  • pmid:16677297
ISSN
1365-2958
DOI
10.1111/j.1365-2958.2006.05134.x
language
English
LU publication?
no
id
5f0bd5fc-15b2-433b-81cb-3e44b02984d8 (old id 770063)
date added to LUP
2016-04-01 11:42:04
date last changed
2020-02-12 03:42:57
@article{5f0bd5fc-15b2-433b-81cb-3e44b02984d8,
  abstract     = {MreB forms a cytoskeleton in many rod-shaped bacteria which is involved in cell shape determination and chromosome segregation. PCR-based and Southern analysis of various actinomycetes, supported by analysis of genome sequences, revealed mreB homologues only in genera that form an aerial mycelium and sporulate. We analysed MreB in one such organism, Streptomyces coelicolor. Ectopic overexpression of mreB impaired growth, and caused swellings and lysis of hyphae. A null mutant with apparently normal vegetative growth was generated. However, aerial hyphae of this mutant were swelling and lysing; spores doubled their volume and lost their characteristic resistance to stress conditions. Loss of cell wall consistency was observed in MreB-depleted spores by transmission electron microscopy. An MreB–EGFP fusion was constructed to localize MreB in the mycelium. No clearly localized signal was seen in vegetative mycelium. However, strong fluorescence was observed at the septa of sporulating aerial hyphae, then as bipolar foci in young spores, and finally in a ring- or shell-like pattern inside the spores. Immunogold electron microscopy using MreB-specific antibodies revealed that MreB is located immediately underneath the internal spore wall. Thus, MreB is not essential for vegetative growth of S. coelicolor, but exerts its function in the formation of environmentally stable spores, and appears to primarily influence the assembly of the spore cell wall.},
  author       = {Mazza, Paola and Noens, Elke E. and Schirner, Kathrin and Grantcharova, Nina and Mommass, A. Mieke and Koerten, Henk K. and Muth, Gunther and Flärdh, Klas and van Wezel, Gilles and Wohlleben, Wolfgang},
  issn         = {1365-2958},
  language     = {eng},
  number       = {4},
  pages        = {838--852},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Microbiology},
  title        = {MreB of Streptomyces coelicolor is not required for vegetative growth but is involved in spore wall synthesis},
  url          = {http://dx.doi.org/10.1111/j.1365-2958.2006.05134.x},
  doi          = {10.1111/j.1365-2958.2006.05134.x},
  volume       = {60},
  year         = {2006},
}