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Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.

Owerbach, D. ; Lernmark, A. LU ; Rask, L. ; Peterson, P. A. ; Platz, P. and Svejgaard, A. (1983) In Proceedings of the National Academy of Sciences of the United States of America 80(12). p.3758-3761
Abstract

Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of... (More)

Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.

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publishing date
type
Contribution to journal
publication status
published
in
Proceedings of the National Academy of Sciences of the United States of America
volume
80
issue
12
pages
4 pages
publisher
National Acad Sciences
external identifiers
  • scopus:0020772532
  • pmid:6304735
ISSN
0027-8424
DOI
10.1073/pnas.80.12.3758
language
English
LU publication?
no
id
5f667838-1000-44a3-b503-79a522887d52
date added to LUP
2019-09-16 12:45:03
date last changed
2021-01-03 05:55:29
@article{5f667838-1000-44a3-b503-79a522887d52,
  abstract     = {<p>Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.</p>},
  author       = {Owerbach, D. and Lernmark, A. and Rask, L. and Peterson, P. A. and Platz, P. and Svejgaard, A.},
  issn         = {0027-8424},
  language     = {eng},
  month        = {01},
  number       = {12},
  pages        = {3758--3761},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences of the United States of America},
  title        = {Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.},
  url          = {http://dx.doi.org/10.1073/pnas.80.12.3758},
  doi          = {10.1073/pnas.80.12.3758},
  volume       = {80},
  year         = {1983},
}