Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.
Owerbach, D. ; Lernmark, A. LU
; Rask, L.
; Peterson, P. A.
; Platz, P.
and Svejgaard, A.
(1983)
In Proceedings of the National Academy of Sciences of the United States of America
80(12).
p.3758-3761
- Abstract
Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of... (More)
Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.
(Less)
- author
- Owerbach, D.
; Lernmark, A.
LU
; Rask, L.
; Peterson, P. A.
; Platz, P.
and Svejgaard, A.
- publishing date
- 1983-01-01
- type
- Contribution to journal
- publication status
- published
- in
- Proceedings of the National Academy of Sciences of the United States of America
- volume
- 80
- issue
- 12
- pages
- 4 pages
- publisher
- National Academy of Sciences
- external identifiers
-
- scopus:0020772532
- pmid:6304735
- ISSN
- 0027-8424
- DOI
- 10.1073/pnas.80.12.3758
- language
- English
- LU publication?
- no
- id
- 5f667838-1000-44a3-b503-79a522887d52
- date added to LUP
- 2019-09-16 12:45:03
- date last changed
- 2025-10-14 13:09:43
@article{5f667838-1000-44a3-b503-79a522887d52,
abstract = {{<p>Sequences of different sizes are generated when DNA from homozygous HLA-Dw/DR typing cells are digested with restriction endonuclease and analyzed by hybridization with a HLA-D region class II antigen beta-chain cDNA probe. The patterns of hybridization were highly polymorphic but one endonuclease, BamHI, defined sequences unique to all HLA-Dw/DR specificities 1-8 except HLA-Dw/DR 2 and 6; however, these two specificities were resolved with the enzyme EcoRI. Digestion with other endonucleases such as Pst I results in patterns of restriction fragments that differ between homozygous typing cells of the same HLA-Dw/DR specificity. HLA-D region beta-chain probes permit HLA-D region genotyping at the DNA level and may allow detection of genes controlling the association of HLA specificities with a wide variety of diseases.</p>}},
author = {{Owerbach, D. and Lernmark, A. and Rask, L. and Peterson, P. A. and Platz, P. and Svejgaard, A.}},
issn = {{0027-8424}},
language = {{eng}},
month = {{01}},
number = {{12}},
pages = {{3758--3761}},
publisher = {{National Academy of Sciences}},
series = {{Proceedings of the National Academy of Sciences of the United States of America}},
title = {{Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells.}},
url = {{http://dx.doi.org/10.1073/pnas.80.12.3758}},
doi = {{10.1073/pnas.80.12.3758}},
volume = {{80}},
year = {{1983}},
}