Quantitative analysis of enzymatic fractionation of multiple substrate mixtures.
Shanker, Shiva LU and Adlercreutz, Patrick LU
(2013)
In Biotechnology and Bioengineering
110(1).
p.78-86
- Abstract
- The enzymatic conversion of mixtures of multiple substrates was studied quantitatively, based on established methodology used for the enzymatic kinetic resolution of racemic mixtures, involving the use of competitive factors: ratios of specificity constants (k(cat) /K(M) ) of substrate pairs. The competitive factors of the substrates were defined in relation to a reference substrate. These competitive factors were used to predict the composition of the reaction mixture as a function of the degree of conversion of the reaction. The methodology was evaluated using three different lipases to hydrolyse a model mixture of four fatty acid methyl esters and for the esterification of a mixture of the same fatty acids in free form with ethanol. In... (More)
- The enzymatic conversion of mixtures of multiple substrates was studied quantitatively, based on established methodology used for the enzymatic kinetic resolution of racemic mixtures, involving the use of competitive factors: ratios of specificity constants (k(cat) /K(M) ) of substrate pairs. The competitive factors of the substrates were defined in relation to a reference substrate. These competitive factors were used to predict the composition of the reaction mixture as a function of the degree of conversion of the reaction. The methodology was evaluated using three different lipases to hydrolyse a model mixture of four fatty acid methyl esters and for the esterification of a mixture of the same fatty acids in free form with ethanol. In most cases, the competitive factors determined from the initial phase of the reactions predicted the product composition during the rest of the reaction very well. The slowest reacting fatty acid was erucic acid (both in free form and as methyl ester), which was thus enriched in the remaining substrate fraction, while the other fatty acids: lauric acid, palmitic acid and oleic acid were converted faster. Simulations of the compositions of reaction mixtures with different values of the competitive factors were carried out to provide an overview of what could be achieved using enzymatic enrichment. Possible applications include reactions involving homologous substrates and mixtures of multiple isomers. The analysis presented provides guidelines that can be useful in the screening and development of enzymes for enzymatic enrichment applications. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2966919
- author
- Shanker, Shiva
LU
and Adlercreutz, Patrick
LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- enzymatic enrichment, enzyme catalysis, competing substrates, lipase
- in
- Biotechnology and Bioengineering
- volume
- 110
- issue
- 1
- pages
- 78 - 86
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000311380200006
- pmid:22811287
- scopus:84869864345
- pmid:22811287
- ISSN
- 1097-0290
- DOI
- 10.1002/bit.24613
- language
- English
- LU publication?
- yes
- id
- 5fec1b14-376a-43be-8ac2-290d87127927 (old id 2966919)
- date added to LUP
- 2016-04-01 09:51:43
- date last changed
- 2022-04-03 23:58:29
@article{5fec1b14-376a-43be-8ac2-290d87127927,
abstract = {{The enzymatic conversion of mixtures of multiple substrates was studied quantitatively, based on established methodology used for the enzymatic kinetic resolution of racemic mixtures, involving the use of competitive factors: ratios of specificity constants (k(cat) /K(M) ) of substrate pairs. The competitive factors of the substrates were defined in relation to a reference substrate. These competitive factors were used to predict the composition of the reaction mixture as a function of the degree of conversion of the reaction. The methodology was evaluated using three different lipases to hydrolyse a model mixture of four fatty acid methyl esters and for the esterification of a mixture of the same fatty acids in free form with ethanol. In most cases, the competitive factors determined from the initial phase of the reactions predicted the product composition during the rest of the reaction very well. The slowest reacting fatty acid was erucic acid (both in free form and as methyl ester), which was thus enriched in the remaining substrate fraction, while the other fatty acids: lauric acid, palmitic acid and oleic acid were converted faster. Simulations of the compositions of reaction mixtures with different values of the competitive factors were carried out to provide an overview of what could be achieved using enzymatic enrichment. Possible applications include reactions involving homologous substrates and mixtures of multiple isomers. The analysis presented provides guidelines that can be useful in the screening and development of enzymes for enzymatic enrichment applications. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc.}},
author = {{Shanker, Shiva and Adlercreutz, Patrick}},
issn = {{1097-0290}},
keywords = {{enzymatic enrichment; enzyme catalysis; competing substrates; lipase}},
language = {{eng}},
number = {{1}},
pages = {{78--86}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology and Bioengineering}},
title = {{Quantitative analysis of enzymatic fractionation of multiple substrate mixtures.}},
url = {{http://dx.doi.org/10.1002/bit.24613}},
doi = {{10.1002/bit.24613}},
volume = {{110}},
year = {{2013}},
}