Analysis of complement biomarkers in systemic sclerosis indicates a distinct pattern in scleroderma renal crisis
Okrój, Marcin LU ; Johansson, Martin LU ; Saxne, Tore LU ; Blom, Anna M. LU
and Hesselstrand, Roger
LU
(2016)
In Arthritis Research and Therapy
18(1).
- Abstract
Background: The complement system has been implicated in pathogenesis of systemic sclerosis (SSc). The goal of the present study was to evaluate improved complement biomarkers in SSc. Methods: The presence of C4d, reflecting activation of the classical/lectin pathways, C3bBbP corresponding to activation of the alternative pathway, and soluble terminal complement complexes (all complement pathways), was measured in plasma samples by enzyme-linked immunosorbent assay and correlated to clinical parameters. The study included 81 patients with limited cutaneous SSc and 41 with diffuse cutaneous SSc, as well as 47 matched healthy controls and 81 patients with rheumatoid arthritis, 22 with psoriatic arthritis and 20 with ankylosing... (More)
Background: The complement system has been implicated in pathogenesis of systemic sclerosis (SSc). The goal of the present study was to evaluate improved complement biomarkers in SSc. Methods: The presence of C4d, reflecting activation of the classical/lectin pathways, C3bBbP corresponding to activation of the alternative pathway, and soluble terminal complement complexes (all complement pathways), was measured in plasma samples by enzyme-linked immunosorbent assay and correlated to clinical parameters. The study included 81 patients with limited cutaneous SSc and 41 with diffuse cutaneous SSc, as well as 47 matched healthy controls and 81 patients with rheumatoid arthritis, 22 with psoriatic arthritis and 20 with ankylosing spondylitis. Skin and kidney biopsies of selected patients were stained to detect deposited C3b as a marker of local complement activation. Results: Biomarkers of activation of all complement pathways were increased in SSc compared with healthy controls and were similar to those in other rheumatic diseases. When patients with SSc were divided into subgroups, a distinct pattern of complement markers was observed in individuals with scleroderma renal crisis (SRC). By functional assay, we confirmed a significant decrease in complement haemolytic activity in SRC vs. non-SRC patients, indicating complement consumption. Further, we detected glomerular deposits of C3b in some patients with SRC. Conclusions: The data indicate that complement activation is an important feature of SRC.
(Less)
- author
- Okrój, Marcin
LU
; Johansson, Martin
LU
; Saxne, Tore
LU
; Blom, Anna M.
LU
and Hesselstrand, Roger
LU
- organization
- publishing date
- 2016-11-18
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biomarkers, Complement, Renal crisis, Scleroderma, Systemic sclerosis
- in
- Arthritis Research and Therapy
- volume
- 18
- issue
- 1
- article number
- 267
- publisher
- BioMed Central (BMC)
- external identifiers
-
- pmid:27863511
- wos:000388416000002
- scopus:84998775143
- ISSN
- 1478-6354
- DOI
- 10.1186/s13075-016-1168-x
- language
- English
- LU publication?
- yes
- id
- 6009cf00-556c-426c-8939-0eb0ebdc80e4
- date added to LUP
- 2016-12-19 12:52:56
- date last changed
- 2024-11-03 11:09:40
@article{6009cf00-556c-426c-8939-0eb0ebdc80e4,
abstract = {{<p>Background: The complement system has been implicated in pathogenesis of systemic sclerosis (SSc). The goal of the present study was to evaluate improved complement biomarkers in SSc. Methods: The presence of C4d, reflecting activation of the classical/lectin pathways, C3bBbP corresponding to activation of the alternative pathway, and soluble terminal complement complexes (all complement pathways), was measured in plasma samples by enzyme-linked immunosorbent assay and correlated to clinical parameters. The study included 81 patients with limited cutaneous SSc and 41 with diffuse cutaneous SSc, as well as 47 matched healthy controls and 81 patients with rheumatoid arthritis, 22 with psoriatic arthritis and 20 with ankylosing spondylitis. Skin and kidney biopsies of selected patients were stained to detect deposited C3b as a marker of local complement activation. Results: Biomarkers of activation of all complement pathways were increased in SSc compared with healthy controls and were similar to those in other rheumatic diseases. When patients with SSc were divided into subgroups, a distinct pattern of complement markers was observed in individuals with scleroderma renal crisis (SRC). By functional assay, we confirmed a significant decrease in complement haemolytic activity in SRC vs. non-SRC patients, indicating complement consumption. Further, we detected glomerular deposits of C3b in some patients with SRC. Conclusions: The data indicate that complement activation is an important feature of SRC.</p>}},
author = {{Okrój, Marcin and Johansson, Martin and Saxne, Tore and Blom, Anna M. and Hesselstrand, Roger}},
issn = {{1478-6354}},
keywords = {{Biomarkers; Complement; Renal crisis; Scleroderma; Systemic sclerosis}},
language = {{eng}},
month = {{11}},
number = {{1}},
publisher = {{BioMed Central (BMC)}},
series = {{Arthritis Research and Therapy}},
title = {{Analysis of complement biomarkers in systemic sclerosis indicates a distinct pattern in scleroderma renal crisis}},
url = {{http://dx.doi.org/10.1186/s13075-016-1168-x}},
doi = {{10.1186/s13075-016-1168-x}},
volume = {{18}},
year = {{2016}},
}