Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis
(2019) In Cell Reports 27(10). p.5-2836- Abstract
The distinct lineage potential is a key feature of hematopoietic stem cell (HSC) heterogeneity, but a subset of HSCs specialized for a single lymphoid compartment has not been identified. Here we report that HSCs expressing junctional adhesion molecule 2 (Jam2) at a higher level (Jam2high HSCs) have a greater T cell reconstitution capacity. Jam2high HSCs are metabolically dormant but preferentially differentiate toward lymphocytes, especially T cell lineages. Jam2high HSCs uniquely express T cell-related genes, and the interaction with Jam1 facilitates the Notch/Delta signaling pathway. Frequency of Jam2high HSCs changes upon T cell depletion in vivo, potentially suggesting that Jam2... (More)
The distinct lineage potential is a key feature of hematopoietic stem cell (HSC) heterogeneity, but a subset of HSCs specialized for a single lymphoid compartment has not been identified. Here we report that HSCs expressing junctional adhesion molecule 2 (Jam2) at a higher level (Jam2high HSCs) have a greater T cell reconstitution capacity. Jam2high HSCs are metabolically dormant but preferentially differentiate toward lymphocytes, especially T cell lineages. Jam2high HSCs uniquely express T cell-related genes, and the interaction with Jam1 facilitates the Notch/Delta signaling pathway. Frequency of Jam2high HSCs changes upon T cell depletion in vivo, potentially suggesting that Jam2 expression may reflect scarcity of T cells and requirement of T cell replenishment. Our findings highlight Jam2 as a potential marker for a subfraction of HSCs with an extensive lymphopoietic capacity, mainly in T lymphopoiesis. Radulovic et al. show that hematopoietic stem cells expressing Jam2 at a higher level on their cell surface (Jam2high HSCs) have a greater lymphopoietic potential, particularly T cells. Interaction with Jam1 facilitates Notch/Delta signals, which might be the potential mechanism. The frequency of Jam2high HSCs changes upon selective hematopoietic stress.
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- author
- Radulovic, Visnja LU ; van der Garde, Mark LU ; Koide, Shuhei LU ; Sigurdsson, Valgardur LU ; Lang, Stefan LU ; Kaneko, Shin and Miharada, Kenichi LU
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- hematopoietic stem cell, Jam2, junctional adhesion molecule, Notch signal, stem cell heterogeneity, T cell
- in
- Cell Reports
- volume
- 27
- issue
- 10
- pages
- 5 - 2836
- publisher
- Cell Press
- external identifiers
-
- scopus:85065758169
- pmid:31167130
- ISSN
- 2211-1247
- DOI
- 10.1016/j.celrep.2019.05.028
- language
- English
- LU publication?
- yes
- id
- 605707a8-8ca7-4e4b-b49c-5b05ac072f09
- date added to LUP
- 2019-06-17 11:31:00
- date last changed
- 2024-04-02 09:57:57
@article{605707a8-8ca7-4e4b-b49c-5b05ac072f09, abstract = {{<p>The distinct lineage potential is a key feature of hematopoietic stem cell (HSC) heterogeneity, but a subset of HSCs specialized for a single lymphoid compartment has not been identified. Here we report that HSCs expressing junctional adhesion molecule 2 (Jam2) at a higher level (Jam2<sup>high</sup> HSCs) have a greater T cell reconstitution capacity. Jam2<sup>high</sup> HSCs are metabolically dormant but preferentially differentiate toward lymphocytes, especially T cell lineages. Jam2<sup>high</sup> HSCs uniquely express T cell-related genes, and the interaction with Jam1 facilitates the Notch/Delta signaling pathway. Frequency of Jam2<sup>high</sup> HSCs changes upon T cell depletion in vivo, potentially suggesting that Jam2 expression may reflect scarcity of T cells and requirement of T cell replenishment. Our findings highlight Jam2 as a potential marker for a subfraction of HSCs with an extensive lymphopoietic capacity, mainly in T lymphopoiesis. Radulovic et al. show that hematopoietic stem cells expressing Jam2 at a higher level on their cell surface (Jam2<sup>high</sup> HSCs) have a greater lymphopoietic potential, particularly T cells. Interaction with Jam1 facilitates Notch/Delta signals, which might be the potential mechanism. The frequency of Jam2<sup>high</sup> HSCs changes upon selective hematopoietic stress.</p>}}, author = {{Radulovic, Visnja and van der Garde, Mark and Koide, Shuhei and Sigurdsson, Valgardur and Lang, Stefan and Kaneko, Shin and Miharada, Kenichi}}, issn = {{2211-1247}}, keywords = {{hematopoietic stem cell; Jam2; junctional adhesion molecule; Notch signal; stem cell heterogeneity; T cell}}, language = {{eng}}, number = {{10}}, pages = {{5--2836}}, publisher = {{Cell Press}}, series = {{Cell Reports}}, title = {{Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis}}, url = {{http://dx.doi.org/10.1016/j.celrep.2019.05.028}}, doi = {{10.1016/j.celrep.2019.05.028}}, volume = {{27}}, year = {{2019}}, }