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P-selectin glycoprotein ligand-1 regulates chemokine-dependent leukocyte recruitment in colonic ischemia-reperfusion.

Santén, Stefan LU ; Schramm, R; Menger, M; Wang, Yusheng LU ; Jeppsson, Bengt LU and Thorlacius, Henrik LU (2007) In Inflammation Research 56(11). p.452-458
Abstract
Objective and design: Leukocyte recruitment is a key feature in ischemia-reperfusion (I/R) -provoked tissue injury. This study evaluated the role of P-selectin-glycoprotein ligand-1 (PSGL-1) in CXC chemokine- and ischemia-reperfusion- induced leukocyte rolling and adhesion in the colon.

Materials Balb/c mice were used in an inverted intravital fluorescence microscopy study of the microvascular bed in the colon.

Treatment: Mice were challenged with macrophage inflammatory protein-2 (MIP-2) intraperitonally and leukocyte-endothelium interactions were analysed 3 h later. In separate experiments, mice were exposed to I/R by clamping of the superior mesenteric artery for 30 min and leukocyte rolling and adhesion were analysed... (More)
Objective and design: Leukocyte recruitment is a key feature in ischemia-reperfusion (I/R) -provoked tissue injury. This study evaluated the role of P-selectin-glycoprotein ligand-1 (PSGL-1) in CXC chemokine- and ischemia-reperfusion- induced leukocyte rolling and adhesion in the colon.

Materials Balb/c mice were used in an inverted intravital fluorescence microscopy study of the microvascular bed in the colon.

Treatment: Mice were challenged with macrophage inflammatory protein-2 (MIP-2) intraperitonally and leukocyte-endothelium interactions were analysed 3 h later. In separate experiments, mice were exposed to I/R by clamping of the superior mesenteric artery for 30 min and leukocyte rolling and adhesion were analysed after 120 min of reperfusion.

Results: MIP-2 dose-dependently increased leukocyte rolling and adhesion in the colon. Pretreatment with an anti-PSGL-1 antibody reduced MIP-2-provoked leukocyte rolling and adhesion by more than 89%. I/R increased expression of MIP-2 as well as leukocyte rolling and adhesion. Immunoneutralization of PSGL-1 decreased reperfusion-induced leukocyte rolling by 85% and adhesion by 93% in colonic venules.

Conclusions: Our data demonstrates that PSGL-1 is a dominant adhesion molecule supporting MIP-2- and I/R-provoked leukocyte rolling. Inhibition of PSGL-1 abolished leukocyte rolling and abrogated I/R-induced leukocyte adhesion in colonic venules. These findings suggest that targeting PSGL-1 may be an effective strategy to prevent I/R-induced inflammation in the colon. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Inflammation Research
volume
56
issue
11
pages
452 - 458
publisher
Birkhaüser
external identifiers
  • wos:000251512300003
  • scopus:38549179966
ISSN
1420-908X
DOI
10.1007/s00011-007-7071-9
language
English
LU publication?
yes
id
28163035-8f5d-46c6-b5bc-e8c2563db619 (old id 607542)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17891336&dopt=Abstract
date added to LUP
2008-08-08 13:29:56
date last changed
2017-04-30 08:45:01
@article{28163035-8f5d-46c6-b5bc-e8c2563db619,
  abstract     = {Objective and design: Leukocyte recruitment is a key feature in ischemia-reperfusion (I/R) -provoked tissue injury. This study evaluated the role of P-selectin-glycoprotein ligand-1 (PSGL-1) in CXC chemokine- and ischemia-reperfusion- induced leukocyte rolling and adhesion in the colon.<br/><br>
Materials Balb/c mice were used in an inverted intravital fluorescence microscopy study of the microvascular bed in the colon.<br/><br>
Treatment: Mice were challenged with macrophage inflammatory protein-2 (MIP-2) intraperitonally and leukocyte-endothelium interactions were analysed 3 h later. In separate experiments, mice were exposed to I/R by clamping of the superior mesenteric artery for 30 min and leukocyte rolling and adhesion were analysed after 120 min of reperfusion.<br/><br>
Results: MIP-2 dose-dependently increased leukocyte rolling and adhesion in the colon. Pretreatment with an anti-PSGL-1 antibody reduced MIP-2-provoked leukocyte rolling and adhesion by more than 89%. I/R increased expression of MIP-2 as well as leukocyte rolling and adhesion. Immunoneutralization of PSGL-1 decreased reperfusion-induced leukocyte rolling by 85% and adhesion by 93% in colonic venules.<br/><br>
Conclusions: Our data demonstrates that PSGL-1 is a dominant adhesion molecule supporting MIP-2- and I/R-provoked leukocyte rolling. Inhibition of PSGL-1 abolished leukocyte rolling and abrogated I/R-induced leukocyte adhesion in colonic venules. These findings suggest that targeting PSGL-1 may be an effective strategy to prevent I/R-induced inflammation in the colon.},
  author       = {Santén, Stefan and Schramm, R and Menger, M and Wang, Yusheng and Jeppsson, Bengt and Thorlacius, Henrik},
  issn         = {1420-908X},
  language     = {eng},
  number       = {11},
  pages        = {452--458},
  publisher    = {Birkhaüser},
  series       = {Inflammation Research},
  title        = {P-selectin glycoprotein ligand-1 regulates chemokine-dependent leukocyte recruitment in colonic ischemia-reperfusion.},
  url          = {http://dx.doi.org/10.1007/s00011-007-7071-9},
  volume       = {56},
  year         = {2007},
}