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First case of type E wound botulism diagnosed using real-time PCR.

Artin, Ingrid LU ; Björkman, Per LU ; Cronqvist, Jonas LU ; Rådström, Peter LU and Holst, Elisabet LU (2007) In Journal of Clinical Microbiology 45(11). p.3589-3594
Abstract
Wound botulism is a growing problem among injecting drug users. The condition is often difficult to diagnose, with laboratory confirmation in only 50% of the cases. Here we present a real-time PCR-based method for the diagnosis of wound botulism caused by Clostridium botulinum. The assay includes an internal amplification control which is amplified simultaneously with the genes encoding neurotoxin types A, B, and E. This method was used to detect the first case of wound botulism in an injecting drug user in Sweden. In addition, to the best of our knowledge, this is the first reported case of wound botulism caused by C. botulinum type E.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Microbiology
volume
45
issue
11
pages
3589 - 3594
publisher
American Society for Microbiology
external identifiers
  • wos:000250932700017
  • scopus:36349011454
ISSN
1098-660X
DOI
10.1128/JCM.01192-07
language
English
LU publication?
yes
id
496e1497-1412-4769-a177-1a74bd258c0d (old id 607648)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17881556&dopt=Abstract
date added to LUP
2007-11-22 17:57:18
date last changed
2017-09-10 04:33:10
@article{496e1497-1412-4769-a177-1a74bd258c0d,
  abstract     = {Wound botulism is a growing problem among injecting drug users. The condition is often difficult to diagnose, with laboratory confirmation in only 50% of the cases. Here we present a real-time PCR-based method for the diagnosis of wound botulism caused by Clostridium botulinum. The assay includes an internal amplification control which is amplified simultaneously with the genes encoding neurotoxin types A, B, and E. This method was used to detect the first case of wound botulism in an injecting drug user in Sweden. In addition, to the best of our knowledge, this is the first reported case of wound botulism caused by C. botulinum type E.},
  author       = {Artin, Ingrid and Björkman, Per and Cronqvist, Jonas and Rådström, Peter and Holst, Elisabet},
  issn         = {1098-660X},
  language     = {eng},
  number       = {11},
  pages        = {3589--3594},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Clinical Microbiology},
  title        = {First case of type E wound botulism diagnosed using real-time PCR.},
  url          = {http://dx.doi.org/10.1128/JCM.01192-07},
  volume       = {45},
  year         = {2007},
}