First case of type E wound botulism diagnosed using real-time PCR.
Artin, Ingrid LU ; Björkman, Per LU
; Cronqvist, Jonas
LU
; Rådström, Peter
LU
and Holst, Elisabet
LU
(2007)
In Journal of Clinical Microbiology
45(11).
p.3589-3594
- Abstract
- Wound botulism is a growing problem among injecting drug users. The condition is often difficult to diagnose, with laboratory confirmation in only 50% of the cases. Here we present a real-time PCR-based method for the diagnosis of wound botulism caused by Clostridium botulinum. The assay includes an internal amplification control which is amplified simultaneously with the genes encoding neurotoxin types A, B, and E. This method was used to detect the first case of wound botulism in an injecting drug user in Sweden. In addition, to the best of our knowledge, this is the first reported case of wound botulism caused by C. botulinum type E.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/607648
- author
- Artin, Ingrid
LU
; Björkman, Per
LU
; Cronqvist, Jonas
LU
; Rådström, Peter
LU
and Holst, Elisabet
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Clinical Microbiology
- volume
- 45
- issue
- 11
- pages
- 3589 - 3594
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000250932700017
- scopus:36349011454
- ISSN
- 1098-660X
- DOI
- 10.1128/JCM.01192-07
- language
- English
- LU publication?
- yes
- id
- 496e1497-1412-4769-a177-1a74bd258c0d (old id 607648)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17881556&dopt=Abstract
- date added to LUP
- 2016-04-01 16:22:36
- date last changed
- 2022-01-28 19:17:43
@article{496e1497-1412-4769-a177-1a74bd258c0d,
abstract = {{Wound botulism is a growing problem among injecting drug users. The condition is often difficult to diagnose, with laboratory confirmation in only 50% of the cases. Here we present a real-time PCR-based method for the diagnosis of wound botulism caused by Clostridium botulinum. The assay includes an internal amplification control which is amplified simultaneously with the genes encoding neurotoxin types A, B, and E. This method was used to detect the first case of wound botulism in an injecting drug user in Sweden. In addition, to the best of our knowledge, this is the first reported case of wound botulism caused by C. botulinum type E.}},
author = {{Artin, Ingrid and Björkman, Per and Cronqvist, Jonas and Rådström, Peter and Holst, Elisabet}},
issn = {{1098-660X}},
language = {{eng}},
number = {{11}},
pages = {{3589--3594}},
publisher = {{American Society for Microbiology}},
series = {{Journal of Clinical Microbiology}},
title = {{First case of type E wound botulism diagnosed using real-time PCR.}},
url = {{http://dx.doi.org/10.1128/JCM.01192-07}},
doi = {{10.1128/JCM.01192-07}},
volume = {{45}},
year = {{2007}},
}