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Microbial manipulation of the rat dam changes bacterial colonization and alters properties of the gut in her offspring.

Fåk, Frida LU ; Ahrné, Siv LU ; Molin, Göran LU ; Jeppsson, Bengt LU and Weström, Björn LU (2008) In American Journal of Physiology: Gastrointestinal and Liver Physiology 294. p.148-154
Abstract
The impact of an altered bacterial colonization on gut development has not been thoroughly studied, despite the increased risk of certain diseases with a disturbed microbiota after birth. This study was conducted to determine the effect of microbial manipulation, i.e. antibiotic treatment or Escherichia coli (E. coli) exposure, of the dam on bacterial colonization and gut development in the offspring. Pregnant rats were administered either broad-spectrum antibiotics three days prior to parturition, or live non-pathogenic E. coli CCUG 29300T one week before parturition and up to 14 days of lactation in the drinking water. Caecal bacterial levels, gut growth, intestinal permeability, digestive enzyme levels and intestinal inflammation were... (More)
The impact of an altered bacterial colonization on gut development has not been thoroughly studied, despite the increased risk of certain diseases with a disturbed microbiota after birth. This study was conducted to determine the effect of microbial manipulation, i.e. antibiotic treatment or Escherichia coli (E. coli) exposure, of the dam on bacterial colonization and gut development in the offspring. Pregnant rats were administered either broad-spectrum antibiotics three days prior to parturition, or live non-pathogenic E. coli CCUG 29300T one week before parturition and up to 14 days of lactation in the drinking water. Caecal bacterial levels, gut growth, intestinal permeability, digestive enzyme levels and intestinal inflammation were studied in two-week old rats. Pups from dams that were antibiotic-treated had higher densities of Enterobacteriaceae which correlated with a decreased stomach growth and function, lower pancreatic protein levels, higher intestinal permeability and increased plasma levels of the acute phase protein, haptoglobin, compared with pups from untreated mothers. Exposure of pregnant/lactating mothers to E. coli CCUG 29300T, also resulting in increased Enterobacteriaceae levels, gave in the offspring similar results on the stomach and an increased small intestinal growth as compared to the control pups. Furthermore, E. coli pups showed increased mucosal disaccharidase activities, increased liver, spleen and adrenal weights, as well as increased plasma concentrations of haptoglobin. These findings indicate that disturbing the normal bacterial colonization after birth, by increasing the densities of caecal Enterobacteriaceae, appear to have lasting effects on the postnatal microflora which affects gut growth and function. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
American Journal of Physiology: Gastrointestinal and Liver Physiology
volume
294
pages
148 - 154
publisher
American Physiological Society
external identifiers
  • wos:000252464600017
  • scopus:38349182439
ISSN
1522-1547
DOI
10.1152/ajpgi.00023.2007
language
English
LU publication?
yes
id
bd5dbde3-4dce-4397-906f-fd5539d5d8dc (old id 608321)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17962363&dopt=Abstract
date added to LUP
2007-12-30 14:36:40
date last changed
2017-09-10 03:39:42
@article{bd5dbde3-4dce-4397-906f-fd5539d5d8dc,
  abstract     = {The impact of an altered bacterial colonization on gut development has not been thoroughly studied, despite the increased risk of certain diseases with a disturbed microbiota after birth. This study was conducted to determine the effect of microbial manipulation, i.e. antibiotic treatment or Escherichia coli (E. coli) exposure, of the dam on bacterial colonization and gut development in the offspring. Pregnant rats were administered either broad-spectrum antibiotics three days prior to parturition, or live non-pathogenic E. coli CCUG 29300T one week before parturition and up to 14 days of lactation in the drinking water. Caecal bacterial levels, gut growth, intestinal permeability, digestive enzyme levels and intestinal inflammation were studied in two-week old rats. Pups from dams that were antibiotic-treated had higher densities of Enterobacteriaceae which correlated with a decreased stomach growth and function, lower pancreatic protein levels, higher intestinal permeability and increased plasma levels of the acute phase protein, haptoglobin, compared with pups from untreated mothers. Exposure of pregnant/lactating mothers to E. coli CCUG 29300T, also resulting in increased Enterobacteriaceae levels, gave in the offspring similar results on the stomach and an increased small intestinal growth as compared to the control pups. Furthermore, E. coli pups showed increased mucosal disaccharidase activities, increased liver, spleen and adrenal weights, as well as increased plasma concentrations of haptoglobin. These findings indicate that disturbing the normal bacterial colonization after birth, by increasing the densities of caecal Enterobacteriaceae, appear to have lasting effects on the postnatal microflora which affects gut growth and function.},
  author       = {Fåk, Frida and Ahrné, Siv and Molin, Göran and Jeppsson, Bengt and Weström, Björn},
  issn         = {1522-1547},
  language     = {eng},
  pages        = {148--154},
  publisher    = {American Physiological Society},
  series       = {American Journal of Physiology: Gastrointestinal and Liver Physiology},
  title        = {Microbial manipulation of the rat dam changes bacterial colonization and alters properties of the gut in her offspring.},
  url          = {http://dx.doi.org/10.1152/ajpgi.00023.2007},
  volume       = {294},
  year         = {2008},
}