Radio-iodinated and internally labelled (35S) IgM monoclonal antibodies in a syngenic rat model
(1991) In Acta Oncologica 30(3). p.379-383- Abstract
- To simulate the human situation concerning human monoclonal antibodies (MAbs), we have introduced a new syngenic rat model with an implanted rat colon carcinoma. Rat IgM MAbs (10B12), labelled by the chloramine-T method with 125I or internally with 35S, were injected intravenously into the rats and the biodistribution was studied for 8 days. The radioactivity uptake in the tumours of the 35S label was higher than that of the 125I label and the retention of 35S in the tumours gave tumour/blood ratios 8 times higher than those of 125I at 48 and 96 h after injection. In this model we have shown that dehalogenation of iodinated IgM MAbs is a serious problem. We therefore suggest that internally labelled MAbs should be used and that further... (More)
- To simulate the human situation concerning human monoclonal antibodies (MAbs), we have introduced a new syngenic rat model with an implanted rat colon carcinoma. Rat IgM MAbs (10B12), labelled by the chloramine-T method with 125I or internally with 35S, were injected intravenously into the rats and the biodistribution was studied for 8 days. The radioactivity uptake in the tumours of the 35S label was higher than that of the 125I label and the retention of 35S in the tumours gave tumour/blood ratios 8 times higher than those of 125I at 48 and 96 h after injection. In this model we have shown that dehalogenation of iodinated IgM MAbs is a serious problem. We therefore suggest that internally labelled MAbs should be used and that further investigations should be carried out in a syngenic rat model, since this probably reflects the clinical situation better than the nude mouse model. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1105770
- author
- Wanying, Q
; Brodin, T
; Ceberg, Crister
LU
; Ingvar, Christian LU ; Norrgren, Kristina LU ; Sjogren, H O and Strand, Sven-Erik LU
- organization
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Monoclonal antibodies, colon carcinoma, rat
- in
- Acta Oncologica
- volume
- 30
- issue
- 3
- pages
- 379 - 383
- publisher
- Taylor & Francis
- external identifiers
-
- pmid:2036250
- scopus:0025790945
- ISSN
- 1651-226X
- DOI
- 10.3109/02841869109092390
- language
- English
- LU publication?
- yes
- id
- 60ae7c3a-2dc4-40a9-9f7b-d4ac88040d85 (old id 1105770)
- date added to LUP
- 2016-04-01 16:59:37
- date last changed
- 2021-01-03 07:12:39
@article{60ae7c3a-2dc4-40a9-9f7b-d4ac88040d85, abstract = {{To simulate the human situation concerning human monoclonal antibodies (MAbs), we have introduced a new syngenic rat model with an implanted rat colon carcinoma. Rat IgM MAbs (10B12), labelled by the chloramine-T method with 125I or internally with 35S, were injected intravenously into the rats and the biodistribution was studied for 8 days. The radioactivity uptake in the tumours of the 35S label was higher than that of the 125I label and the retention of 35S in the tumours gave tumour/blood ratios 8 times higher than those of 125I at 48 and 96 h after injection. In this model we have shown that dehalogenation of iodinated IgM MAbs is a serious problem. We therefore suggest that internally labelled MAbs should be used and that further investigations should be carried out in a syngenic rat model, since this probably reflects the clinical situation better than the nude mouse model.}}, author = {{Wanying, Q and Brodin, T and Ceberg, Crister and Ingvar, Christian and Norrgren, Kristina and Sjogren, H O and Strand, Sven-Erik}}, issn = {{1651-226X}}, keywords = {{Monoclonal antibodies; colon carcinoma; rat}}, language = {{eng}}, number = {{3}}, pages = {{379--383}}, publisher = {{Taylor & Francis}}, series = {{Acta Oncologica}}, title = {{Radio-iodinated and internally labelled (35S) IgM monoclonal antibodies in a syngenic rat model}}, url = {{http://dx.doi.org/10.3109/02841869109092390}}, doi = {{10.3109/02841869109092390}}, volume = {{30}}, year = {{1991}}, }