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The porcine large intestine contains developmentally distinct submucosal lymphoid clusters and mucosal isolated lymphoid follicles

Jørgensen, Peter B ; Eriksen, Lise L ; Fenton, Thomas M ; Bailey, Michael ; Agace, William W LU and Mörbe, Urs M LU (2022) In Developmental and Comparative Immunology 131.
Abstract

Gut-associated lymphoid tissues (GALT) serve as key priming sites for intestinal adaptive immune responses. Most of our understanding of GALT function and development arises from studies in mice. However, the diversity, structure and cellular composition of GALT differs markedly between mammalian species and the developmental window in which distinct GALT structures develop in large mammals remains poorly understood. Given the importance of pigs as models of human disease, as well as their role in livestock production, we adapted a recently developed protocol for the isolation of human GALT to assess the diversity, development and immune composition of large intestinal GALT in neonatal and adult pigs. We demonstrate that the large... (More)

Gut-associated lymphoid tissues (GALT) serve as key priming sites for intestinal adaptive immune responses. Most of our understanding of GALT function and development arises from studies in mice. However, the diversity, structure and cellular composition of GALT differs markedly between mammalian species and the developmental window in which distinct GALT structures develop in large mammals remains poorly understood. Given the importance of pigs as models of human disease, as well as their role in livestock production, we adapted a recently developed protocol for the isolation of human GALT to assess the diversity, development and immune composition of large intestinal GALT in neonatal and adult pigs. We demonstrate that the large intestine of adult pigs contains two major GALT types; multifollicular submucosal GALT that we term submucosal lymphoid clusters (SLC) which develop prenatally, and as yet undescribed mucosal isolated lymphoid follicles (M-ILF), which arise after birth. Using confocal laser microscopy and flow cytometry, we additionally assess the microanatomy and lymphocyte composition of SLC and M-ILF, compare them to jejunal Peyer's patches (PP), and describe the maturation of these structures. Collectively, our results provide a deeper understanding of the diversity and development of GALT within the porcine large intestine.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Developmental and Comparative Immunology
volume
131
article number
104375
publisher
Elsevier
external identifiers
  • pmid:35219758
  • scopus:85125476752
ISSN
0145-305X
DOI
10.1016/j.dci.2022.104375
language
English
LU publication?
yes
id
610b4275-e662-481b-94bf-f8addbc1694d
date added to LUP
2022-03-04 18:13:29
date last changed
2024-06-13 16:08:29
@article{610b4275-e662-481b-94bf-f8addbc1694d,
  abstract     = {{<p>Gut-associated lymphoid tissues (GALT) serve as key priming sites for intestinal adaptive immune responses. Most of our understanding of GALT function and development arises from studies in mice. However, the diversity, structure and cellular composition of GALT differs markedly between mammalian species and the developmental window in which distinct GALT structures develop in large mammals remains poorly understood. Given the importance of pigs as models of human disease, as well as their role in livestock production, we adapted a recently developed protocol for the isolation of human GALT to assess the diversity, development and immune composition of large intestinal GALT in neonatal and adult pigs. We demonstrate that the large intestine of adult pigs contains two major GALT types; multifollicular submucosal GALT that we term submucosal lymphoid clusters (SLC) which develop prenatally, and as yet undescribed mucosal isolated lymphoid follicles (M-ILF), which arise after birth. Using confocal laser microscopy and flow cytometry, we additionally assess the microanatomy and lymphocyte composition of SLC and M-ILF, compare them to jejunal Peyer's patches (PP), and describe the maturation of these structures. Collectively, our results provide a deeper understanding of the diversity and development of GALT within the porcine large intestine.</p>}},
  author       = {{Jørgensen, Peter B and Eriksen, Lise L and Fenton, Thomas M and Bailey, Michael and Agace, William W and Mörbe, Urs M}},
  issn         = {{0145-305X}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Developmental and Comparative Immunology}},
  title        = {{The porcine large intestine contains developmentally distinct submucosal lymphoid clusters and mucosal isolated lymphoid follicles}},
  url          = {{http://dx.doi.org/10.1016/j.dci.2022.104375}},
  doi          = {{10.1016/j.dci.2022.104375}},
  volume       = {{131}},
  year         = {{2022}},
}