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Improved MALDI-TOF-MS analysis of partially depolymerised methyl cellulose by reducing end derivatisation

Momcilovic, Dane LU ; Brinkmalm, Gunnar; Wittgren, Bengt and Wahlund, Karl-Gustav LU (2002) Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics In Proceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics p.421-422
Abstract
The depolymerized methyl cellulose was analyzed, using improved MALDI-TOF-MS by reducing the end derivatization. The methyl cellulose was depolymerized to give methylated oligo-(1->4)-β-D-anhydroglucoses (M-oligo-AG). To reduce the errors during measurement of degree of substitution (DS), M-oligo-AG was derivatized with Girard's T reagent performed on an equimolar mixture of maltooligosaccharides. The GT-derivatized M-oligo-AG (GT-M-oligo-AG) formed singly charged molecular ions with peaks at m/z values 91 higher than the corresponding peaks of M-oligo-AG. The results show that for GT-M-oligo-AG the relative signal intensities for DP=1 and 2 were increased significantly.
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Methyl cellulose, Matrix-assisted laser desorption/ionization (MALDI), Time-of flight (TOF) mass spectrometry, Maltooligosaccharides
in
Proceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics
pages
421 - 422
publisher
American Society for Mass Spectrometry, Santa Fe, NM, 87505, United States
conference name
Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
external identifiers
  • Scopus:2442684602
language
English
LU publication?
yes
id
2945fa3b-48e5-4531-8453-2bf6e9e6a081 (old id 611749)
date added to LUP
2007-11-28 11:39:13
date last changed
2017-01-01 08:07:09
@inproceedings{2945fa3b-48e5-4531-8453-2bf6e9e6a081,
  abstract     = {The depolymerized methyl cellulose was analyzed, using improved MALDI-TOF-MS by reducing the end derivatization. The methyl cellulose was depolymerized to give methylated oligo-(1->4)-β-D-anhydroglucoses (M-oligo-AG). To reduce the errors during measurement of degree of substitution (DS), M-oligo-AG was derivatized with Girard's T reagent performed on an equimolar mixture of maltooligosaccharides. The GT-derivatized M-oligo-AG (GT-M-oligo-AG) formed singly charged molecular ions with peaks at m/z values 91 higher than the corresponding peaks of M-oligo-AG. The results show that for GT-M-oligo-AG the relative signal intensities for DP=1 and 2 were increased significantly.},
  author       = {Momcilovic, Dane and Brinkmalm, Gunnar and Wittgren, Bengt and Wahlund, Karl-Gustav},
  booktitle    = {Proceedings 50th ASMS Conference on Mass Spectrmetry and Allied Topics},
  keyword      = {Methyl cellulose,Matrix-assisted laser desorption/ionization (MALDI),Time-of flight (TOF) mass spectrometry,Maltooligosaccharides},
  language     = {eng},
  pages        = {421--422},
  publisher    = {American Society for Mass Spectrometry, Santa Fe, NM, 87505, United States},
  title        = {Improved MALDI-TOF-MS analysis of partially depolymerised methyl cellulose by reducing end derivatisation},
  year         = {2002},
}