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Versatile microchip utilising ultrasonic manipulation of microparticles

Nilsson, M; Lilliehorn, T; Johansson, L; Almqvist, Monica LU ; Simu, U; Johansson, S; Laurell, Thomas LU and Nilsson, Johan LU (2005) Proceedings of the International Federation for Medical & Biomedical Engineering. 13th Nordic Baltic Conference on Biomedical Engineering and Medical Physics In Proceedings of the International Federation for Medical & Biomedical Engineering. 13th Nordic Baltic Conference on Biomedical Engineering and Medical Physics p.123-124
Abstract
This paper presents the concept and initial work on a microfluidic platform for bead-based analysis of biological sample. The core technology in this project is ultrasonic manipulation and trapping of particle in array configurations by means of acoustic forces. The platform is ultimately aimed for parallel multistep bioassays performed on biochemically activated microbeads (or particles) using submicrolitre sample volumes. A first prototype with three individually controlled particle trapping sites has been developed and evaluated. Standing ultrasonic waves were generated across a microfluidic channel by integrated PZT ultrasonic microtransducers. Particles in a fluid passing a transducer were drawn to pressure minima in the acoustic... (More)
This paper presents the concept and initial work on a microfluidic platform for bead-based analysis of biological sample. The core technology in this project is ultrasonic manipulation and trapping of particle in array configurations by means of acoustic forces. The platform is ultimately aimed for parallel multistep bioassays performed on biochemically activated microbeads (or particles) using submicrolitre sample volumes. A first prototype with three individually controlled particle trapping sites has been developed and evaluated. Standing ultrasonic waves were generated across a microfluidic channel by integrated PZT ultrasonic microtransducers. Particles in a fluid passing a transducer were drawn to pressure minima in the acoustic field, thereby being trapped and confined laterally over the transducer. It is anticipated that acoustic trapping using integrated transducers can be exploited in miniaturised total chemical analysis systems (μTAS), where e.g. microbeads with immobilised antibodies can be trapped in arrays and subjected to minute amounts of sample followed by a reaction, detected using fluorescence. Preliminary results indicate that the platform is capable of handling live cells as well as microbeads. A first model bioassay with detection of fluorescein marked avidin binding to trapped biotin beads has been evaluated (Less)
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author
organization
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Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
acoustic field, integrated PZT ultrasonic microtransducer, microfluidic channel, standing ultrasonic wave, submicrolitre sample volume, biochemically activated microbead, parallel multistep bioassay, biological sample, microparticle, versatile microchip, ultrasonic manipulation, acoustic trapping, chemical analysis system, immobilised antibody, fluorescein marked avidin binding, biotin bead
in
Proceedings of the International Federation for Medical & Biomedical Engineering. 13th Nordic Baltic Conference on Biomedical Engineering and Medical Physics
pages
123 - 124
publisher
Int. Federation for Medical and Biological Eng
conference name
Proceedings of the International Federation for Medical & Biomedical Engineering. 13th Nordic Baltic Conference on Biomedical Engineering and Medical Physics
ISBN
91-7305-910-2
language
English
LU publication?
yes
id
9ae042d4-8eac-470f-8b2a-e3ea15a767df (old id 615254)
date added to LUP
2007-11-25 15:42:01
date last changed
2016-04-16 07:46:30
@inproceedings{9ae042d4-8eac-470f-8b2a-e3ea15a767df,
  abstract     = {This paper presents the concept and initial work on a microfluidic platform for bead-based analysis of biological sample. The core technology in this project is ultrasonic manipulation and trapping of particle in array configurations by means of acoustic forces. The platform is ultimately aimed for parallel multistep bioassays performed on biochemically activated microbeads (or particles) using submicrolitre sample volumes. A first prototype with three individually controlled particle trapping sites has been developed and evaluated. Standing ultrasonic waves were generated across a microfluidic channel by integrated PZT ultrasonic microtransducers. Particles in a fluid passing a transducer were drawn to pressure minima in the acoustic field, thereby being trapped and confined laterally over the transducer. It is anticipated that acoustic trapping using integrated transducers can be exploited in miniaturised total chemical analysis systems (μTAS), where e.g. microbeads with immobilised antibodies can be trapped in arrays and subjected to minute amounts of sample followed by a reaction, detected using fluorescence. Preliminary results indicate that the platform is capable of handling live cells as well as microbeads. A first model bioassay with detection of fluorescein marked avidin binding to trapped biotin beads has been evaluated},
  author       = {Nilsson, M and Lilliehorn, T and Johansson, L and Almqvist, Monica and Simu, U and Johansson, S and Laurell, Thomas and Nilsson, Johan},
  booktitle    = {Proceedings of the International Federation for Medical & Biomedical Engineering. 13th Nordic Baltic Conference on Biomedical Engineering and Medical Physics},
  isbn         = {91-7305-910-2},
  keyword      = {acoustic field,integrated PZT ultrasonic microtransducer,microfluidic channel,standing ultrasonic wave,submicrolitre sample volume,biochemically activated microbead,parallel multistep bioassay,biological sample,microparticle,versatile microchip,ultrasonic manipulation,acoustic trapping,chemical analysis system,immobilised antibody,fluorescein marked avidin binding,biotin bead},
  language     = {eng},
  pages        = {123--124},
  publisher    = {Int. Federation for Medical and Biological Eng},
  title        = {Versatile microchip utilising ultrasonic manipulation of microparticles},
  year         = {2005},
}