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Scatter correction of transmission NIR spectra by photon migration data - Quantitative analysis of solids

Abrahamsson, Christoffer LU ; Andersson-Engels, Stefan LU ; Svanberg, Sune LU ; Johansson, Jonas and Folestad, Staffan (2005) Optical Methods in Drug Discovery and Development In Proceedings of SPIE - The International Society for Optical Engineering 6009. p.60090-60090
Abstract
The scope of this presentation is a new methodology to correct conventional NIR data for scattering effects. The technique aims at measuring the absorption coefficient of the samples rather than the total attenuation, measured by conventional NIR spectroscopy. The main advantage of this is that the absorption coefficient is independent of the path length of the light inside the sample, and therefore independent of the scattering effects. The measurements in this work were made using a novel system for time-resolved measurements, based on short light continuum pulses generated in an index-guided crystal fibre and a spectrometer-equipped streak camera. The system enables spectral recordings in the wavelength range 500 - 1200 nm with a... (More)
The scope of this presentation is a new methodology to correct conventional NIR data for scattering effects. The technique aims at measuring the absorption coefficient of the samples rather than the total attenuation, measured by conventional NIR spectroscopy. The main advantage of this is that the absorption coefficient is independent of the path length of the light inside the sample, and therefore independent of the scattering effects. The measurements in this work were made using a novel system for time-resolved measurements, based on short light continuum pulses generated in an index-guided crystal fibre and a spectrometer-equipped streak camera. The system enables spectral recordings in the wavelength range 500 - 1200 nm with a spectral resolution of 5 nm and a temporal resolution of 30 ps. The evaluation scheme is based on modeling of light transport by diffusion theory, that provides an independent measure of the scattering properties of the samples, that later is used to correct conventional NIR data. This yields a clear advantage over other pre-processing techniques, where scattering effects are estimated and corrected for by using the shape of the measured spectrum only. PLS calibration models shows that, by using the proposed evaluation scheme, the predictive ability is improved by 50% as compared to models based on conventional NIR data. The method also makes it possible to predict the concentration of active substance in samples with physical properties different from those of the samples included in the calibration model. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
NIR spectroscopy, Scatter correction, Time-resolved spectroscopy, Photon migration
in
Proceedings of SPIE - The International Society for Optical Engineering
volume
6009
pages
60090 - 60090
publisher
International Society for Optical Engineering
conference name
Optical Methods in Drug Discovery and Development
external identifiers
  • scopus:33644760458
ISSN
0277-786X
1996-756X
DOI
10.1117/12.638173
language
English
LU publication?
yes
id
ec72985b-09ba-4b09-ae24-f0c939adf0b8 (old id 616513)
date added to LUP
2007-11-22 15:59:51
date last changed
2017-01-01 04:37:18
@inproceedings{ec72985b-09ba-4b09-ae24-f0c939adf0b8,
  abstract     = {The scope of this presentation is a new methodology to correct conventional NIR data for scattering effects. The technique aims at measuring the absorption coefficient of the samples rather than the total attenuation, measured by conventional NIR spectroscopy. The main advantage of this is that the absorption coefficient is independent of the path length of the light inside the sample, and therefore independent of the scattering effects. The measurements in this work were made using a novel system for time-resolved measurements, based on short light continuum pulses generated in an index-guided crystal fibre and a spectrometer-equipped streak camera. The system enables spectral recordings in the wavelength range 500 - 1200 nm with a spectral resolution of 5 nm and a temporal resolution of 30 ps. The evaluation scheme is based on modeling of light transport by diffusion theory, that provides an independent measure of the scattering properties of the samples, that later is used to correct conventional NIR data. This yields a clear advantage over other pre-processing techniques, where scattering effects are estimated and corrected for by using the shape of the measured spectrum only. PLS calibration models shows that, by using the proposed evaluation scheme, the predictive ability is improved by 50% as compared to models based on conventional NIR data. The method also makes it possible to predict the concentration of active substance in samples with physical properties different from those of the samples included in the calibration model.},
  author       = {Abrahamsson, Christoffer and Andersson-Engels, Stefan and Svanberg, Sune and Johansson, Jonas and Folestad, Staffan},
  booktitle    = {Proceedings of SPIE - The International Society for Optical Engineering},
  issn         = {0277-786X},
  keyword      = {NIR spectroscopy,Scatter correction,Time-resolved spectroscopy,Photon migration},
  language     = {eng},
  pages        = {60090--60090},
  publisher    = {International Society for Optical Engineering},
  title        = {Scatter correction of transmission NIR spectra by photon migration data - Quantitative analysis of solids},
  url          = {http://dx.doi.org/10.1117/12.638173},
  volume       = {6009},
  year         = {2005},
}