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Stratum corneum hydration: phase transformations and mobility in stratum corneum, extracted lipids and isolated corneocytes

Silva, Claudia L ; Topgaard, Daniel LU ; Kocherbitov, Vitaly LU ; Sousa, J.J.S. ; Pais, Alberto ACC and Sparr, Emma LU (2007) In Biochimica et Biophysica Acta - Biomembranes 1768(11). p.2647-2659
Abstract
The outermost layer of skin, stratum corneum (SC), functions as the major barrier to diffusion. SC has the architecture of dead keratin filled

cells embedded in a lipid matrix. This work presents a detailed study of the hydration process in extracted SC lipids, isolated corneocytes and

intact SC. Using isothermal sorption microcalorimetry and relaxation and wideline 1H NMR, we study these systems at varying degrees of

hydration/relative humidities (RH) at 25 °C. The basic findings are (i) there is a substantial swelling both of SC lipids, the corneocytes and the

intact SC at high RH. At low RHs corneocytes take up more water than SC lipids do, while at high RHs swelling of SC lipids is more... (More)
The outermost layer of skin, stratum corneum (SC), functions as the major barrier to diffusion. SC has the architecture of dead keratin filled

cells embedded in a lipid matrix. This work presents a detailed study of the hydration process in extracted SC lipids, isolated corneocytes and

intact SC. Using isothermal sorption microcalorimetry and relaxation and wideline 1H NMR, we study these systems at varying degrees of

hydration/relative humidities (RH) at 25 °C. The basic findings are (i) there is a substantial swelling both of SC lipids, the corneocytes and the

intact SC at high RH. At low RHs corneocytes take up more water than SC lipids do, while at high RHs swelling of SC lipids is more pronounced

than that of corneocytes. (ii) Lipids in a fluid state are present in both extracted SC lipids and in the intact SC. (iii) The fraction of fluid lipids is

lower at 1.4% water content than at 15% but remains virtually constant as the water content is further increased. (iv) Three exothermic phase

transitions are detected in the SC lipids at RH=91–94%, and we speculate that the lipid re-organization is responsible for the hydration-induced

variations in SC permeability. (v) The hydration causes swelling in the corneocytes, while it does not affect the mobility of solid components

(keratin filaments). (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
SC, Sorption microcalorimetry, Molecular mobility, Corneocyte, Lipid, Wideline NMR, Phase behavior
in
Biochimica et Biophysica Acta - Biomembranes
volume
1768
issue
11
pages
2647 - 2659
publisher
Elsevier
external identifiers
  • wos:000251493700001
  • scopus:35848966805
  • pmid:17927949
ISSN
0005-2736
DOI
10.1016/j.bbamem.2007.05.028
language
English
LU publication?
yes
id
8665722e-5b23-4a3c-94da-54025c921c0d (old id 619159)
date added to LUP
2016-04-01 16:16:46
date last changed
2022-04-07 07:03:57
@article{8665722e-5b23-4a3c-94da-54025c921c0d,
  abstract     = {{The outermost layer of skin, stratum corneum (SC), functions as the major barrier to diffusion. SC has the architecture of dead keratin filled<br/><br>
cells embedded in a lipid matrix. This work presents a detailed study of the hydration process in extracted SC lipids, isolated corneocytes and<br/><br>
intact SC. Using isothermal sorption microcalorimetry and relaxation and wideline 1H NMR, we study these systems at varying degrees of<br/><br>
hydration/relative humidities (RH) at 25 °C. The basic findings are (i) there is a substantial swelling both of SC lipids, the corneocytes and the<br/><br>
intact SC at high RH. At low RHs corneocytes take up more water than SC lipids do, while at high RHs swelling of SC lipids is more pronounced<br/><br>
than that of corneocytes. (ii) Lipids in a fluid state are present in both extracted SC lipids and in the intact SC. (iii) The fraction of fluid lipids is<br/><br>
lower at 1.4% water content than at 15% but remains virtually constant as the water content is further increased. (iv) Three exothermic phase<br/><br>
transitions are detected in the SC lipids at RH=91–94%, and we speculate that the lipid re-organization is responsible for the hydration-induced<br/><br>
variations in SC permeability. (v) The hydration causes swelling in the corneocytes, while it does not affect the mobility of solid components<br/><br>
(keratin filaments).}},
  author       = {{Silva, Claudia L and Topgaard, Daniel and Kocherbitov, Vitaly and Sousa, J.J.S. and Pais, Alberto ACC and Sparr, Emma}},
  issn         = {{0005-2736}},
  keywords     = {{SC; Sorption microcalorimetry; Molecular mobility; Corneocyte; Lipid; Wideline NMR; Phase behavior}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{2647--2659}},
  publisher    = {{Elsevier}},
  series       = {{Biochimica et Biophysica Acta - Biomembranes}},
  title        = {{Stratum corneum hydration: phase transformations and mobility in stratum corneum, extracted lipids and isolated corneocytes}},
  url          = {{http://dx.doi.org/10.1016/j.bbamem.2007.05.028}},
  doi          = {{10.1016/j.bbamem.2007.05.028}},
  volume       = {{1768}},
  year         = {{2007}},
}