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Evidence for substrate binding of a recombinant thermostable xylanase originating from Rhodothermus marinus

Nordberg Karlsson, Eva LU orcid ; Bartonek-Roxå, Eva LU and Holst, Olle LU (1998) In FEMS Microbiology Letters 168(1). p.1-7
Abstract

The xyn1 encoded 5 domain xylanase from the thermophilic bacterium Rhodothermus marinus binds specifically to xylan, β-glucan and amorphous but not crystalline cellulose. Our results show that the binding is mediated by the full length xylanase, but not by the catalytic domain only. Based on similarities concerning both predicted secondary structure and binding specificity found with one cellulose binding domain of CenC from Cellulomonas fimi, we suggest that the binding is mediated by the two N-terminally repeated domains. Copyright (C) 1998 Federation of European Microbiological Societies.

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Carbohydrate binding domain, Rhodothermus marinus, Thermostable, Xylanase
in
FEMS Microbiology Letters
volume
168
issue
1
pages
7 pages
publisher
Oxford University Press
external identifiers
  • pmid:9812357
  • scopus:0032211894
ISSN
0378-1097
DOI
10.1016/S0378-1097(98)00404-2
language
English
LU publication?
yes
id
61b1a91e-54f5-44d0-8bd6-40096aae0579
date added to LUP
2018-11-14 21:15:09
date last changed
2024-04-15 16:37:30
@article{61b1a91e-54f5-44d0-8bd6-40096aae0579,
  abstract     = {{<p>The xyn1 encoded 5 domain xylanase from the thermophilic bacterium Rhodothermus marinus binds specifically to xylan, β-glucan and amorphous but not crystalline cellulose. Our results show that the binding is mediated by the full length xylanase, but not by the catalytic domain only. Based on similarities concerning both predicted secondary structure and binding specificity found with one cellulose binding domain of CenC from Cellulomonas fimi, we suggest that the binding is mediated by the two N-terminally repeated domains. Copyright (C) 1998 Federation of European Microbiological Societies.</p>}},
  author       = {{Nordberg Karlsson, Eva and Bartonek-Roxå, Eva and Holst, Olle}},
  issn         = {{0378-1097}},
  keywords     = {{Carbohydrate binding domain; Rhodothermus marinus; Thermostable; Xylanase}},
  language     = {{eng}},
  month        = {{11}},
  number       = {{1}},
  pages        = {{1--7}},
  publisher    = {{Oxford University Press}},
  series       = {{FEMS Microbiology Letters}},
  title        = {{Evidence for substrate binding of a recombinant thermostable xylanase originating from Rhodothermus marinus}},
  url          = {{http://dx.doi.org/10.1016/S0378-1097(98)00404-2}},
  doi          = {{10.1016/S0378-1097(98)00404-2}},
  volume       = {{168}},
  year         = {{1998}},
}