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Analysis of complexes formed by small gold nanoparticles in low concentration in cell culture media

Gunnarsson, Stefán B. LU ; Bernfur, Katja LU ; Englund-Johansson, Ulrica LU ; Johansson, Fredrik LU and Cedervall, Tommy LU (2019) In PLoS ONE 14(6).
Abstract

New nanomaterials are constantly developed with applications in everything from cosmetics to high tech electronics. Assessing their biological impact has been done by analysis of their adsorbed protein corona, in vitro cell assays, and larger scale ecotoxicological studies. This has proved to be a huge challenge due to the wide range of available nanomaterials and their unpredictable behaviour in different environments. Furthermore, the enormous number of experimental variables make comparisons difficult. Concentration is one of these variables and can vary greatly depending on the aim of the study. When analysing the protein corona, concentrations are often higher than in cell assays. Using a combination of complementary techniques, we... (More)

New nanomaterials are constantly developed with applications in everything from cosmetics to high tech electronics. Assessing their biological impact has been done by analysis of their adsorbed protein corona, in vitro cell assays, and larger scale ecotoxicological studies. This has proved to be a huge challenge due to the wide range of available nanomaterials and their unpredictable behaviour in different environments. Furthermore, the enormous number of experimental variables make comparisons difficult. Concentration is one of these variables and can vary greatly depending on the aim of the study. When analysing the protein corona, concentrations are often higher than in cell assays. Using a combination of complementary techniques, we have characterised 20 nm gold nanoparticles in a concentration level commonly used in cell studies. We compare their behaviour in a commonly used, protein rich medium and one protein poor medium over 24 hours. Under these conditions, the NPs were stable in protein rich environment but underwent gradual aggregation in protein poor medium. We characterise the biomolecular corona in both media. In protein poor medium, we can describe the often overlooked aggregation. The aggregates' morphology is confirmed by cryo-TEM. Finally, in the protein poor medium, by infrared spectroscopy, we have identified the amino acid arginine in the biomolecular corona which drives the aggregation.

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organization
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Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
14
issue
6
publisher
Public Library of Science
external identifiers
  • scopus:85067420507
ISSN
1932-6203
DOI
10.1371/journal.pone.0218211
language
English
LU publication?
yes
id
62163523-3c81-4576-8af3-25eb341f7ef8
date added to LUP
2019-07-05 10:58:30
date last changed
2019-07-30 05:06:05
@article{62163523-3c81-4576-8af3-25eb341f7ef8,
  abstract     = {<p>New nanomaterials are constantly developed with applications in everything from cosmetics to high tech electronics. Assessing their biological impact has been done by analysis of their adsorbed protein corona, in vitro cell assays, and larger scale ecotoxicological studies. This has proved to be a huge challenge due to the wide range of available nanomaterials and their unpredictable behaviour in different environments. Furthermore, the enormous number of experimental variables make comparisons difficult. Concentration is one of these variables and can vary greatly depending on the aim of the study. When analysing the protein corona, concentrations are often higher than in cell assays. Using a combination of complementary techniques, we have characterised 20 nm gold nanoparticles in a concentration level commonly used in cell studies. We compare their behaviour in a commonly used, protein rich medium and one protein poor medium over 24 hours. Under these conditions, the NPs were stable in protein rich environment but underwent gradual aggregation in protein poor medium. We characterise the biomolecular corona in both media. In protein poor medium, we can describe the often overlooked aggregation. The aggregates' morphology is confirmed by cryo-TEM. Finally, in the protein poor medium, by infrared spectroscopy, we have identified the amino acid arginine in the biomolecular corona which drives the aggregation.</p>},
  articleno    = {e0218211},
  author       = {Gunnarsson, Stefán B. and Bernfur, Katja and Englund-Johansson, Ulrica and Johansson, Fredrik and Cedervall, Tommy},
  issn         = {1932-6203},
  language     = {eng},
  number       = {6},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Analysis of complexes formed by small gold nanoparticles in low concentration in cell culture media},
  url          = {http://dx.doi.org/10.1371/journal.pone.0218211},
  volume       = {14},
  year         = {2019},
}