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Biofunctionalization of dispense-plotted hydroxyapatite scaffolds with peptides : Quantification and cellular response

Detsch, Rainer ; Dieser, Irina ; Deisinger, Ulrike ; Uhl, Franziska LU ; Hamisch, Sabine ; Ziegler, Günter and Lipps, Georg (2010) In Journal of Biomedical Materials Research - Part A 92(2). p.493-503
Abstract

Hydroxyapatite (HA) ceramic is a widely used synthetic bone substitute material for the regeneration of bone defects. We manufactured HA scaffolds with adjustable pore sizes and pore geometry by dispense-plotting. In addition, we attached peptides covalently onto the HA surface and are able to simultaneously quantify the amount of covalently attached and adsorbed peptide down to the picomolar range with a novel fluorescence-based detection method. In cell culture assays with stromal bone marrow cells, we observed a positive effect of biofunctionalization on cell differentiation after 21 days of culture when comparing the scaffold functionalized with the RGD motif containing adhesion peptide to an unmodified scaffold.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Biofunctionalization, Bone marrow cells, Bone substitute, Hydroxyapatite, Osteoblastic differentiation, Rapid prototyping
in
Journal of Biomedical Materials Research - Part A
volume
92
issue
2
pages
11 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:75149141713
  • pmid:19213057
ISSN
1549-3296
DOI
10.1002/jbm.a.32386
language
English
LU publication?
no
id
622386c3-8d54-4344-a01d-f62d456f4342
date added to LUP
2018-09-13 17:54:54
date last changed
2024-10-15 07:13:59
@article{622386c3-8d54-4344-a01d-f62d456f4342,
  abstract     = {{<p>Hydroxyapatite (HA) ceramic is a widely used synthetic bone substitute material for the regeneration of bone defects. We manufactured HA scaffolds with adjustable pore sizes and pore geometry by dispense-plotting. In addition, we attached peptides covalently onto the HA surface and are able to simultaneously quantify the amount of covalently attached and adsorbed peptide down to the picomolar range with a novel fluorescence-based detection method. In cell culture assays with stromal bone marrow cells, we observed a positive effect of biofunctionalization on cell differentiation after 21 days of culture when comparing the scaffold functionalized with the RGD motif containing adhesion peptide to an unmodified scaffold.</p>}},
  author       = {{Detsch, Rainer and Dieser, Irina and Deisinger, Ulrike and Uhl, Franziska and Hamisch, Sabine and Ziegler, Günter and Lipps, Georg}},
  issn         = {{1549-3296}},
  keywords     = {{Biofunctionalization; Bone marrow cells; Bone substitute; Hydroxyapatite; Osteoblastic differentiation; Rapid prototyping}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{2}},
  pages        = {{493--503}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Journal of Biomedical Materials Research - Part A}},
  title        = {{Biofunctionalization of dispense-plotted hydroxyapatite scaffolds with peptides : Quantification and cellular response}},
  url          = {{http://dx.doi.org/10.1002/jbm.a.32386}},
  doi          = {{10.1002/jbm.a.32386}},
  volume       = {{92}},
  year         = {{2010}},
}