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Composition of carbon, nitrogen and phosphorus in single cells of three filamentous cyanobacteria using nuclear microprobe and traditional techniques

Blanco, Eva Perez ; Karlsson, Chatarina ; Pallon, Jan LU and Graneli, Edna (2013) In Aquatic Microbial Ecology 71(1). p.91-97
Abstract
Intracellular of carbon, nitrogen and phosphorus content in phytoplankton cells is traditionally measured using concentrates containing thousands to millions of cells. In the present study, we used a nuclear microprobe (NMP) to determine C, N and P concentrations in single filaments of 3 cyanobacteria species: Anabaena sp., Nodularia spumigena and Aphanizomenon flosaquae var. klebahnii isolated from Baltic Sea water. Estimations of C, N and P content per cell were calculated and compared to the concentrations found using traditional bulk methods. No significant differences regarding the C, N and P cellular content were found between the 2 methods for each of the species tested (p < 0.05). From our results, we conclude that the NMP can... (More)
Intracellular of carbon, nitrogen and phosphorus content in phytoplankton cells is traditionally measured using concentrates containing thousands to millions of cells. In the present study, we used a nuclear microprobe (NMP) to determine C, N and P concentrations in single filaments of 3 cyanobacteria species: Anabaena sp., Nodularia spumigena and Aphanizomenon flosaquae var. klebahnii isolated from Baltic Sea water. Estimations of C, N and P content per cell were calculated and compared to the concentrations found using traditional bulk methods. No significant differences regarding the C, N and P cellular content were found between the 2 methods for each of the species tested (p < 0.05). From our results, we conclude that the NMP can be a useful tool for studying the elemental content of single phytoplankton cells. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Baltic Sea, Elemental cellular content, Nitrogen-fixing cyanobacteria, Nuclear microprobe, Nutrients
in
Aquatic Microbial Ecology
volume
71
issue
1
pages
91 - 97
publisher
Inter-Research
external identifiers
  • wos:000327552900007
  • scopus:84889071168
ISSN
0948-3055
DOI
10.3354/ame01665
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Nuclear Physics (Faculty of Technology) (011013007)
id
622bc627-a381-48db-9646-b7ba3028d463 (old id 4275334)
date added to LUP
2016-04-01 13:28:04
date last changed
2022-01-27 19:19:50
@article{622bc627-a381-48db-9646-b7ba3028d463,
  abstract     = {{Intracellular of carbon, nitrogen and phosphorus content in phytoplankton cells is traditionally measured using concentrates containing thousands to millions of cells. In the present study, we used a nuclear microprobe (NMP) to determine C, N and P concentrations in single filaments of 3 cyanobacteria species: Anabaena sp., Nodularia spumigena and Aphanizomenon flosaquae var. klebahnii isolated from Baltic Sea water. Estimations of C, N and P content per cell were calculated and compared to the concentrations found using traditional bulk methods. No significant differences regarding the C, N and P cellular content were found between the 2 methods for each of the species tested (p &lt; 0.05). From our results, we conclude that the NMP can be a useful tool for studying the elemental content of single phytoplankton cells.}},
  author       = {{Blanco, Eva Perez and Karlsson, Chatarina and Pallon, Jan and Graneli, Edna}},
  issn         = {{0948-3055}},
  keywords     = {{Baltic Sea; Elemental cellular content; Nitrogen-fixing cyanobacteria; Nuclear microprobe; Nutrients}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{91--97}},
  publisher    = {{Inter-Research}},
  series       = {{Aquatic Microbial Ecology}},
  title        = {{Composition of carbon, nitrogen and phosphorus in single cells of three filamentous cyanobacteria using nuclear microprobe and traditional techniques}},
  url          = {{http://dx.doi.org/10.3354/ame01665}},
  doi          = {{10.3354/ame01665}},
  volume       = {{71}},
  year         = {{2013}},
}