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Clonal chromosome aberrations are present in vivo in synovia and osteophytes from patients with osteoarthritis

Broberg, K LU ; Limon, J; Pålsson, E LU ; Lindstrand, A LU ; Toksvig-Larsen, S LU ; Mandahl, N LU and Mertens, F LU (1997) In Human Genetics 101(3). p.8-295
Abstract

We have previously reported recurrent clonal chromosomal aberrations in synovia, osteophytes and articular cartilage from patients with osteoarthritis (OA). In particular, gain of chromosomes 5 and 7 was found to be strongly associated with OA. In order to exclude the possibility of in vitro artefacts, we studied three to four parallel, independent cultures from ten samples of synovia and three samples of osteophytes from ten women with primary OA. In all, 40 cultures were cytogenetically analysed, 39 of which had clonal chromosomal aberrations. The most common aberrations were +7 and +5 which were found in 38 and 12 cultures, respectively. There were striking karyotype similarities among the parallel cultures from each case. Out of a... (More)

We have previously reported recurrent clonal chromosomal aberrations in synovia, osteophytes and articular cartilage from patients with osteoarthritis (OA). In particular, gain of chromosomes 5 and 7 was found to be strongly associated with OA. In order to exclude the possibility of in vitro artefacts, we studied three to four parallel, independent cultures from ten samples of synovia and three samples of osteophytes from ten women with primary OA. In all, 40 cultures were cytogenetically analysed, 39 of which had clonal chromosomal aberrations. The most common aberrations were +7 and +5 which were found in 38 and 12 cultures, respectively. There were striking karyotype similarities among the parallel cultures from each case. Out of a total of 83 clones, only 11 were unique for one culture, 7 from synovia and 4 from osteophytes. The genetic homogeneity among different cultures from the same patients excludes the possibility of in vitro artefacts and indicates a widespread distribution of the cytogenetically aberrant clones in vivo.

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publication status
published
subject
keywords
Aged, Aged, 80 and over, Bone and Bones/pathology, Chromosome Aberrations, Chromosomes, Human, Pair 5, Chromosomes, Human, Pair 7, Clone Cells, Cytogenetics, Female, Humans, Karyotyping, Knee Joint/pathology, Osteoarthritis/genetics, Osteoblasts/pathology, Synovial Membrane/pathology
in
Human Genetics
volume
101
issue
3
pages
4 pages
publisher
Springer
external identifiers
  • scopus:0031433063
ISSN
0340-6717
DOI
10.1007/s004390050631
language
English
LU publication?
yes
id
625d5e43-df39-439c-b7d3-6b360ab7858b
date added to LUP
2019-02-08 13:59:51
date last changed
2019-02-20 11:49:45
@article{625d5e43-df39-439c-b7d3-6b360ab7858b,
  abstract     = {<p>We have previously reported recurrent clonal chromosomal aberrations in synovia, osteophytes and articular cartilage from patients with osteoarthritis (OA). In particular, gain of chromosomes 5 and 7 was found to be strongly associated with OA. In order to exclude the possibility of in vitro artefacts, we studied three to four parallel, independent cultures from ten samples of synovia and three samples of osteophytes from ten women with primary OA. In all, 40 cultures were cytogenetically analysed, 39 of which had clonal chromosomal aberrations. The most common aberrations were +7 and +5 which were found in 38 and 12 cultures, respectively. There were striking karyotype similarities among the parallel cultures from each case. Out of a total of 83 clones, only 11 were unique for one culture, 7 from synovia and 4 from osteophytes. The genetic homogeneity among different cultures from the same patients excludes the possibility of in vitro artefacts and indicates a widespread distribution of the cytogenetically aberrant clones in vivo.</p>},
  author       = {Broberg, K and Limon, J and Pålsson, E and Lindstrand, A and Toksvig-Larsen, S and Mandahl, N and Mertens, F},
  issn         = {0340-6717},
  keyword      = {Aged,Aged, 80 and over,Bone and Bones/pathology,Chromosome Aberrations,Chromosomes, Human, Pair 5,Chromosomes, Human, Pair 7,Clone Cells,Cytogenetics,Female,Humans,Karyotyping,Knee Joint/pathology,Osteoarthritis/genetics,Osteoblasts/pathology,Synovial Membrane/pathology},
  language     = {eng},
  number       = {3},
  pages        = {8--295},
  publisher    = {Springer},
  series       = {Human Genetics},
  title        = {Clonal chromosome aberrations are present in vivo in synovia and osteophytes from patients with osteoarthritis},
  url          = {http://dx.doi.org/10.1007/s004390050631},
  volume       = {101},
  year         = {1997},
}