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A unique symbiosome in an anaerobic single-celled eukaryote

Jerlström-Hultqvist, Jon ; Gallot-Lavallée, Lucie ; Salas-Leiva, Dayana E. ; Curtis, Bruce A. ; Záhonová, Kristína ; Čepička, Ivan ; Stairs, Courtney W. LU orcid ; Pipaliya, Shweta ; Dacks, Joel B. and Archibald, John M. , et al. (2024) In Nature Communications 15.
Abstract

Symbiotic relationships between eukaryotes and prokaryotes played pivotal roles in the evolution of life and drove the emergence of specialized symbiotic structures in animals, plants and fungi. The host-evolved symbiotic structures of microbial eukaryotes – the vast majority of such hosts in nature – remain largely unstudied. Here we describe highly structured symbiosomes within three free-living anaerobic protists (Anaeramoeba spp.). We dissect this symbiosis using complete genome sequencing and transcriptomics of host and symbiont cells coupled with fluorescence in situ hybridization, and 3D reconstruction using focused-ion-beam scanning electron microscopy. The emergence of the symbiosome is underpinned by expansion of gene families... (More)

Symbiotic relationships between eukaryotes and prokaryotes played pivotal roles in the evolution of life and drove the emergence of specialized symbiotic structures in animals, plants and fungi. The host-evolved symbiotic structures of microbial eukaryotes – the vast majority of such hosts in nature – remain largely unstudied. Here we describe highly structured symbiosomes within three free-living anaerobic protists (Anaeramoeba spp.). We dissect this symbiosis using complete genome sequencing and transcriptomics of host and symbiont cells coupled with fluorescence in situ hybridization, and 3D reconstruction using focused-ion-beam scanning electron microscopy. The emergence of the symbiosome is underpinned by expansion of gene families encoding regulators of membrane trafficking and phagosomal maturation and extensive bacteria-to-eukaryote lateral transfer. The symbionts reside deep within a symbiosomal membrane network that enables metabolic syntrophy by precisely positioning sulfate-reducing bacteria alongside host hydrogenosomes. Importantly, the symbionts maintain connections to the Anaeramoeba plasma membrane, blurring traditional boundaries between ecto- and endosymbiosis.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nature Communications
volume
15
article number
9726
publisher
Nature Publishing Group
external identifiers
  • scopus:85209474863
  • pmid:39521804
ISSN
2041-1723
DOI
10.1038/s41467-024-54102-7
language
English
LU publication?
yes
additional info
Publisher Copyright: © The Author(s) 2024.
id
62930c98-de6e-4464-bdf2-b0068a2c9040
date added to LUP
2024-12-15 10:29:58
date last changed
2025-05-04 20:25:39
@article{62930c98-de6e-4464-bdf2-b0068a2c9040,
  abstract     = {{<p>Symbiotic relationships between eukaryotes and prokaryotes played pivotal roles in the evolution of life and drove the emergence of specialized symbiotic structures in animals, plants and fungi. The host-evolved symbiotic structures of microbial eukaryotes – the vast majority of such hosts in nature – remain largely unstudied. Here we describe highly structured symbiosomes within three free-living anaerobic protists (Anaeramoeba spp.). We dissect this symbiosis using complete genome sequencing and transcriptomics of host and symbiont cells coupled with fluorescence in situ hybridization, and 3D reconstruction using focused-ion-beam scanning electron microscopy. The emergence of the symbiosome is underpinned by expansion of gene families encoding regulators of membrane trafficking and phagosomal maturation and extensive bacteria-to-eukaryote lateral transfer. The symbionts reside deep within a symbiosomal membrane network that enables metabolic syntrophy by precisely positioning sulfate-reducing bacteria alongside host hydrogenosomes. Importantly, the symbionts maintain connections to the Anaeramoeba plasma membrane, blurring traditional boundaries between ecto- and endosymbiosis.</p>}},
  author       = {{Jerlström-Hultqvist, Jon and Gallot-Lavallée, Lucie and Salas-Leiva, Dayana E. and Curtis, Bruce A. and Záhonová, Kristína and Čepička, Ivan and Stairs, Courtney W. and Pipaliya, Shweta and Dacks, Joel B. and Archibald, John M. and Roger, Andrew J.}},
  issn         = {{2041-1723}},
  language     = {{eng}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Communications}},
  title        = {{A unique symbiosome in an anaerobic single-celled eukaryote}},
  url          = {{http://dx.doi.org/10.1038/s41467-024-54102-7}},
  doi          = {{10.1038/s41467-024-54102-7}},
  volume       = {{15}},
  year         = {{2024}},
}