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Fibronectin promotes cell cycle entry in smooth muscle cells in primary culture

Roy, Joy ; Tran, Phan Kiet LU ; Religa, Piotr ; Kazi, Monsur ; Henderson, Bimma ; Lundmark, Karin LU and Hedin, Ulf (2002) In Experimental Cell Research 273(2). p.169-177
Abstract


Smooth muscle cell proliferation after arterial injury is regulated by growth factors and components of the extracellular matrix. We have previously demonstrated that fibronectin promotes a phenotypic modulation of freshly isolated rat smooth muscle cells from a contractile to a synthetic phenotype in primary culture and supports the ability of the cells to respond to growth factors. Here, we analyzed if fibronectin promotes cell cycle entry in freshly isolated rat aortic smooth muscle cells during primary culture. Cell cycle analysis showed that cells seeded on fibronectin remained in the G
0
/G
... (More)


Smooth muscle cell proliferation after arterial injury is regulated by growth factors and components of the extracellular matrix. We have previously demonstrated that fibronectin promotes a phenotypic modulation of freshly isolated rat smooth muscle cells from a contractile to a synthetic phenotype in primary culture and supports the ability of the cells to respond to growth factors. Here, we analyzed if fibronectin promotes cell cycle entry in freshly isolated rat aortic smooth muscle cells during primary culture. Cell cycle analysis showed that cells seeded on fibronectin remained in the G
0
/G
1
phase of the cell cycle during the first 6 days of culture. During this period, there was an increased expression of cyclin D1 and p27
KIP1
in the absence of exogenous growth factors. Addition of serum was followed by enhanced cyclin D1 expression, decreased p27
KIP1
levels, hyperphosphorylation of Rb protein, induction of cyclin A and cyclin D3 expression, and cell cycle progression into S phase. The results indicate that fibronectin initiates cell cycle entry in freshly isolated smooth muscle cells by promoting the induction of cyclin D1 and thereby facilitates further cell cycle progression together with growth factors.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Cell cycle, Cyclin D1, Extracellular matrix, Fibronectin, Phenotypic modulation, Smooth muscle cells
in
Experimental Cell Research
volume
273
issue
2
pages
9 pages
publisher
Academic Press
external identifiers
  • pmid:11822872
  • scopus:0036059340
ISSN
0014-4827
DOI
10.1006/excr.2001.5427
language
English
LU publication?
no
id
62dfd1ab-bf9e-4d36-9031-b3b5854d5e8b
date added to LUP
2019-07-01 22:52:36
date last changed
2024-01-01 15:15:48
@article{62dfd1ab-bf9e-4d36-9031-b3b5854d5e8b,
  abstract     = {{<p><br>
                            Smooth muscle cell proliferation after arterial injury is regulated by growth factors and components of the extracellular matrix. We have previously demonstrated that fibronectin promotes a phenotypic modulation of freshly isolated rat smooth muscle cells from a contractile to a synthetic phenotype in primary culture and supports the ability of the cells to respond to growth factors. Here, we analyzed if fibronectin promotes cell cycle entry in freshly isolated rat aortic smooth muscle cells during primary culture. Cell cycle analysis showed that cells seeded on fibronectin remained in the G<br>
                            <sub>0</sub><br>
                            /G<br>
                            <sub>1</sub><br>
                             phase of the cell cycle during the first 6 days of culture. During this period, there was an increased expression of cyclin D1 and p27<br>
                            <sup>KIP1</sup><br>
                             in the absence of exogenous growth factors. Addition of serum was followed by enhanced cyclin D1 expression, decreased p27<br>
                            <sup>KIP1</sup><br>
                             levels, hyperphosphorylation of Rb protein, induction of cyclin A and cyclin D3 expression, and cell cycle progression into S phase. The results indicate that fibronectin initiates cell cycle entry in freshly isolated smooth muscle cells by promoting the induction of cyclin D1 and thereby facilitates further cell cycle progression together with growth factors.</p>}},
  author       = {{Roy, Joy and Tran, Phan Kiet and Religa, Piotr and Kazi, Monsur and Henderson, Bimma and Lundmark, Karin and Hedin, Ulf}},
  issn         = {{0014-4827}},
  keywords     = {{Cell cycle; Cyclin D1; Extracellular matrix; Fibronectin; Phenotypic modulation; Smooth muscle cells}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{2}},
  pages        = {{169--177}},
  publisher    = {{Academic Press}},
  series       = {{Experimental Cell Research}},
  title        = {{Fibronectin promotes cell cycle entry in smooth muscle cells in primary culture}},
  url          = {{http://dx.doi.org/10.1006/excr.2001.5427}},
  doi          = {{10.1006/excr.2001.5427}},
  volume       = {{273}},
  year         = {{2002}},
}