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Lack of respiratory chain Complex I impairs alternative oxidase engagement and modulates redox signaling during elicitor-induced cell death in tobacco.

Vidal, Guillaume; Ribas-Carbo, Miquel; Garmier, Marie; Dubertret, Guy; Rasmusson, Allan LU ; Mathieu, Chantal; Foyer, Christine H and De Paepe, Rosine (2007) In Plant Cell 19(2). p.640-655
Abstract
Alternative oxidase (AOX) functions in stress resistance by preventing accumulation of reactive oxygen species (ROS), but little is known about in vivo partitioning of electron flow between AOX and the cytochrome pathway. We investigated the relationships between AOX expression and in vivo activity in Nicotiana sylvestris and the complex I–deficient CMSII mutant in response to a cell death elicitor. While a specific AOX1 isoform in the active reduced state was constitutively overexpressed in CMSII, partitioning through the alternative pathway was similar to the wild type. Lack of correlation between AOX content and activity indicates severe metabolic constraints in nonstressed mutant leaves. The bacterial elicitor harpin NEa induced... (More)
Alternative oxidase (AOX) functions in stress resistance by preventing accumulation of reactive oxygen species (ROS), but little is known about in vivo partitioning of electron flow between AOX and the cytochrome pathway. We investigated the relationships between AOX expression and in vivo activity in Nicotiana sylvestris and the complex I–deficient CMSII mutant in response to a cell death elicitor. While a specific AOX1 isoform in the active reduced state was constitutively overexpressed in CMSII, partitioning through the alternative pathway was similar to the wild type. Lack of correlation between AOX content and activity indicates severe metabolic constraints in nonstressed mutant leaves. The bacterial elicitor harpin NEa induced similar timing and extent of cell death and a twofold respiratory burst in both genotypes with little change in AOX amounts. However, partitioning to AOX was increased twofold in the wild type but remained unchanged in CMSII. Oxidative phosphorylation modeling indicated a twofold ATP increase in both genotypes. By contrast, mitochondrial superoxide dismutase activity and reduced forms of ascorbate and glutathione were higher in CMSII than in the wild type. These results demonstrate genetically programmed flexibility of plant respiratory routes and antioxidants in response to elicitors and suggest that sustained ATP production, rather than AOX activity by itself or mitochondrial ROS, might be important for in planta cell death. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant Cell
volume
19
issue
2
pages
640 - 655
publisher
American Society of Plant Biologists
external identifiers
  • wos:000245467700023
  • scopus:34250672678
ISSN
1040-4651
DOI
10.1105/tpc.106.044461
language
English
LU publication?
yes
id
83f1dcf8-f240-4409-9b92-d08b9d971b94 (old id 637157)
date added to LUP
2007-11-30 14:16:35
date last changed
2017-10-22 03:45:03
@article{83f1dcf8-f240-4409-9b92-d08b9d971b94,
  abstract     = {Alternative oxidase (AOX) functions in stress resistance by preventing accumulation of reactive oxygen species (ROS), but little is known about in vivo partitioning of electron flow between AOX and the cytochrome pathway. We investigated the relationships between AOX expression and in vivo activity in Nicotiana sylvestris and the complex I–deficient CMSII mutant in response to a cell death elicitor. While a specific AOX1 isoform in the active reduced state was constitutively overexpressed in CMSII, partitioning through the alternative pathway was similar to the wild type. Lack of correlation between AOX content and activity indicates severe metabolic constraints in nonstressed mutant leaves. The bacterial elicitor harpin NEa induced similar timing and extent of cell death and a twofold respiratory burst in both genotypes with little change in AOX amounts. However, partitioning to AOX was increased twofold in the wild type but remained unchanged in CMSII. Oxidative phosphorylation modeling indicated a twofold ATP increase in both genotypes. By contrast, mitochondrial superoxide dismutase activity and reduced forms of ascorbate and glutathione were higher in CMSII than in the wild type. These results demonstrate genetically programmed flexibility of plant respiratory routes and antioxidants in response to elicitors and suggest that sustained ATP production, rather than AOX activity by itself or mitochondrial ROS, might be important for in planta cell death.},
  author       = {Vidal, Guillaume and Ribas-Carbo, Miquel and Garmier, Marie and Dubertret, Guy and Rasmusson, Allan and Mathieu, Chantal and Foyer, Christine H and De Paepe, Rosine},
  issn         = {1040-4651},
  language     = {eng},
  number       = {2},
  pages        = {640--655},
  publisher    = {American Society of Plant Biologists},
  series       = {Plant Cell},
  title        = {Lack of respiratory chain Complex I impairs alternative oxidase engagement and modulates redox signaling during elicitor-induced cell death in tobacco.},
  url          = {http://dx.doi.org/10.1105/tpc.106.044461},
  volume       = {19},
  year         = {2007},
}