Advanced

Acoustic Differential Extraction - ultrasonic DNA-extraction from sexual assault evidence

Evander, Mikael LU ; Horsman, Katie M.; Easley, Christopher J.; Landers, James P.; Laurell, Thomas LU and Nilsson, Johan LU (2007) International Congress on Ultrasonics 2007 In International Congress on Ultrasonics 1. p.151-151
Abstract
Isolation of the male and female DNA is one of the most important steps in obtaining the DNA profile of the perpetrator in sexual assault cases. The sample is obtained by taking a vaginal swab containing both epithelial cells from the female and sperm cells from the male from the victim. The purity of the extracted male fraction decides whether or not a single-source DNA profile of the suspect will be obtained or not. The existing techniques are have poor separation efficiency, time-consuming, labour-intensive and are neither easily automated nor integrated with further analysis steps.

A novel method of DNA extraction based on ultrasonic trapping, Acoustic Differential Extraction, has been developed. A microfluidic device using... (More)
Isolation of the male and female DNA is one of the most important steps in obtaining the DNA profile of the perpetrator in sexual assault cases. The sample is obtained by taking a vaginal swab containing both epithelial cells from the female and sperm cells from the male from the victim. The purity of the extracted male fraction decides whether or not a single-source DNA profile of the suspect will be obtained or not. The existing techniques are have poor separation efficiency, time-consuming, labour-intensive and are neither easily automated nor integrated with further analysis steps.

A novel method of DNA extraction based on ultrasonic trapping, Acoustic Differential Extraction, has been developed. A microfluidic device using laminar flow valving and miniature PZT transducers retains the sperm cells while mobilizing the female fraction into a separate outlet. The device was evaluated using a mock sexual assault sample and the separated fractions were analyzed using quantitative PCR and STR-profiling.

A 16-fold enrichment of the male fraction, making an originally hard-to-detect-male DNA profile readily profiled, has been demonstrated. The STR-profiling of the male and female fractions showed a male fraction purity of up to 99 % making it possible to obtain a single-source DNA-profile of the suspect. The microfluidic format of the device makes it possible to downscale the sample time from 4-8 hours to 10 minutes. It is also possible to integrate this method with further downstream analysis steps necessarey for the full forensic DNA analysis. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
in
International Congress on Ultrasonics
editor
Benes, Ewald and
volume
1
pages
1 pages
publisher
International Congress on Ultrasonics
conference name
International Congress on Ultrasonics 2007
language
English
LU publication?
yes
id
c0905344-c05a-4e2f-b2fa-f67c632086be (old id 637872)
date added to LUP
2007-12-21 09:48:57
date last changed
2016-04-16 08:35:52
@inproceedings{c0905344-c05a-4e2f-b2fa-f67c632086be,
  abstract     = {Isolation of the male and female DNA is one of the most important steps in obtaining the DNA profile of the perpetrator in sexual assault cases. The sample is obtained by taking a vaginal swab containing both epithelial cells from the female and sperm cells from the male from the victim. The purity of the extracted male fraction decides whether or not a single-source DNA profile of the suspect will be obtained or not. The existing techniques are have poor separation efficiency, time-consuming, labour-intensive and are neither easily automated nor integrated with further analysis steps. <br/><br>
 A novel method of DNA extraction based on ultrasonic trapping, Acoustic Differential Extraction, has been developed. A microfluidic device using laminar flow valving and miniature PZT transducers retains the sperm cells while mobilizing the female fraction into a separate outlet. The device was evaluated using a mock sexual assault sample and the separated fractions were analyzed using quantitative PCR and STR-profiling.<br/><br>
 A 16-fold enrichment of the male fraction, making an originally hard-to-detect-male DNA profile readily profiled, has been demonstrated. The STR-profiling of the male and female fractions showed a male fraction purity of up to 99 % making it possible to obtain a single-source DNA-profile of the suspect. The microfluidic format of the device makes it possible to downscale the sample time from 4-8 hours to 10 minutes. It is also possible to integrate this method with further downstream analysis steps necessarey for the full forensic DNA analysis.},
  author       = {Evander, Mikael and Horsman, Katie M. and Easley, Christopher J. and Landers, James P. and Laurell, Thomas and Nilsson, Johan},
  booktitle    = {International Congress on Ultrasonics},
  editor       = {Benes, Ewald},
  language     = {eng},
  pages        = {151--151},
  publisher    = {International Congress on Ultrasonics},
  title        = {Acoustic Differential Extraction - ultrasonic DNA-extraction from sexual assault evidence},
  volume       = {1},
  year         = {2007},
}