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Applications of optical DNA mapping in microbiology

Bogas, Diana; Nyberg, Lena; Pacheco, Rui; Azevedo, Nuno F.; Beech, Jason P. LU ; Gomila, Margarita; Lalucat, Jorge; Manaia, Célia M.; Nunes, Olga C. and Tegenfeldt, Jonas O. LU , et al. (2017) In BioTechniques 62(6). p.255-267
Abstract

Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the... (More)

Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the integrated and automated microbiological analysis of biological fluids. Here, we review the basic technology of OM, detail the current state of the art of the field, and look ahead to possible future developments in OM technology for microbiological applications.

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publication status
published
subject
keywords
DNA-based identification, Imaging technique, Lab-on-a chip, Microbiological clinical diagnosis, Optical DNA mapping
in
BioTechniques
volume
62
issue
6
pages
13 pages
publisher
Informa Healthcare
external identifiers
  • scopus:85020897312
  • wos:000403563300004
ISSN
0736-6205
DOI
10.2144/000114555
language
English
LU publication?
yes
id
639288bb-26a0-4ef2-976e-380f8699d475
date added to LUP
2017-07-05 09:35:54
date last changed
2017-09-18 11:37:48
@article{639288bb-26a0-4ef2-976e-380f8699d475,
  abstract     = {<p>Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches. In addition, designing lab-on-a-chip systems based on OM is now feasible and will allow the integrated and automated microbiological analysis of biological fluids. Here, we review the basic technology of OM, detail the current state of the art of the field, and look ahead to possible future developments in OM technology for microbiological applications.</p>},
  author       = {Bogas, Diana and Nyberg, Lena and Pacheco, Rui and Azevedo, Nuno F. and Beech, Jason P. and Gomila, Margarita and Lalucat, Jorge and Manaia, Célia M. and Nunes, Olga C. and Tegenfeldt, Jonas O. and Westerlund, Fredrik},
  issn         = {0736-6205},
  keyword      = {DNA-based identification,Imaging technique,Lab-on-a chip,Microbiological clinical diagnosis,Optical DNA mapping},
  language     = {eng},
  number       = {6},
  pages        = {255--267},
  publisher    = {Informa Healthcare},
  series       = {BioTechniques},
  title        = {Applications of optical DNA mapping in microbiology},
  url          = {http://dx.doi.org/10.2144/000114555},
  volume       = {62},
  year         = {2017},
}