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Electrophysiological and behavioral responses of Ips duplicatus to aggregation pheromone in inner mongolia, China: Amitinol as a potential pheromone component

Zhang, Qing-He; Schlyter, Fredrik; Liu, Guang-Tian; Sheng, Mao-Ling and Birgersson, Göran LU (2007) In Journal of Chemical Ecology 33(7). p.1303-1315
Abstract
Volatiles from Ips duplicatus male hindgut extracts and aeration samples of spruce logs colonized by the virgin males were analyzed by coupled gas chromatography-mass spectrometry (GC-MS), coupled gas chromatography-electroantennographic detection (GC-EAD), and field trapping bioassays in Inner Mongolia, China. GC-MS analyses showed that males from nuptial chambers (for reproduction) produced hindgut volatile profiles similar to those of maturation feeding males from the nonreproductive galleries. The known aggregation pheromone components, ipsdienol (Id) and E-myrcenol (EM) are the major constituents of this blend, followed by several minor bark beetle-related compounds: 2-methyl-3-buten-2-ol, ipsenol, cis-verbenol, verbenone,... (More)
Volatiles from Ips duplicatus male hindgut extracts and aeration samples of spruce logs colonized by the virgin males were analyzed by coupled gas chromatography-mass spectrometry (GC-MS), coupled gas chromatography-electroantennographic detection (GC-EAD), and field trapping bioassays in Inner Mongolia, China. GC-MS analyses showed that males from nuptial chambers (for reproduction) produced hindgut volatile profiles similar to those of maturation feeding males from the nonreproductive galleries. The known aggregation pheromone components, ipsdienol (Id) and E-myrcenol (EM) are the major constituents of this blend, followed by several minor bark beetle-related compounds: 2-methyl-3-buten-2-ol, ipsenol, cis-verbenol, verbenone, 2-phenylethanol, myrtenol, and trans-myrtanol. The enantiomeric composition of the Id from the male hindgut extracts was determined to be a racemic mixture (50/50) by enantioselective GC. GC-EAD experiments indicated that I. duplicatus antennae from both sexes responded strongly not only to the known aggregation pheromone components, Id and EM, but also to the other minor conspecific-produced compounds. The strongest antennal response was elicited by the major pheromone component, Id, which was ca. 2-3 times higher than that elicited by the second component, EM. Electroantennogram dose-responses indicated that antennal response threshold to Id was approximately 100 times lower than that of EM. No synergistic effects between these occurred at peripheral level. A strong and repeatable EAD response was found to a trace compound (flame ionization detection undetectable) from both hindgut and aeration samples. The compound was identified as amitinol (At) based on the retention time and a further EAD analysis of a synthetic mixture. In the field trapping experiments, At increased the attraction of I. duplicatus to the traps baited with a binary blend of EM/Id (1:1) in a synergistic manner when tested at a low release rate (1:1:0.1) but had no effect on catches at the high release rate (1:1:1). This result suggests that At might be a part of the Chinese I. duplicatus aggregation pheromone system. The production ratios of EM/Id from the Inner Mongolian population (EM/Id approximate to 1:2.0-4) were twice as high as those reported from Europe (EM/Id approximate to 1:9), corresponding well with the differences in the optimal response ratios. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
scolytidae, enantiomeric composition, Ipsenol, amitinol, attractant, GC-EAD, Ipsdienol, GC-MS, coleoptera, E-myrcenol
in
Journal of Chemical Ecology
volume
33
issue
7
pages
1303 - 1315
publisher
Springer
external identifiers
  • wos:000247785600002
  • scopus:33846428617
ISSN
1573-1561
DOI
10.1007/s10886-007-9320-3
language
English
LU publication?
yes
id
479aec70-6e7a-4e5c-9293-f307ae432b88 (old id 645689)
date added to LUP
2007-12-21 14:13:01
date last changed
2017-01-01 07:10:05
@article{479aec70-6e7a-4e5c-9293-f307ae432b88,
  abstract     = {Volatiles from Ips duplicatus male hindgut extracts and aeration samples of spruce logs colonized by the virgin males were analyzed by coupled gas chromatography-mass spectrometry (GC-MS), coupled gas chromatography-electroantennographic detection (GC-EAD), and field trapping bioassays in Inner Mongolia, China. GC-MS analyses showed that males from nuptial chambers (for reproduction) produced hindgut volatile profiles similar to those of maturation feeding males from the nonreproductive galleries. The known aggregation pheromone components, ipsdienol (Id) and E-myrcenol (EM) are the major constituents of this blend, followed by several minor bark beetle-related compounds: 2-methyl-3-buten-2-ol, ipsenol, cis-verbenol, verbenone, 2-phenylethanol, myrtenol, and trans-myrtanol. The enantiomeric composition of the Id from the male hindgut extracts was determined to be a racemic mixture (50/50) by enantioselective GC. GC-EAD experiments indicated that I. duplicatus antennae from both sexes responded strongly not only to the known aggregation pheromone components, Id and EM, but also to the other minor conspecific-produced compounds. The strongest antennal response was elicited by the major pheromone component, Id, which was ca. 2-3 times higher than that elicited by the second component, EM. Electroantennogram dose-responses indicated that antennal response threshold to Id was approximately 100 times lower than that of EM. No synergistic effects between these occurred at peripheral level. A strong and repeatable EAD response was found to a trace compound (flame ionization detection undetectable) from both hindgut and aeration samples. The compound was identified as amitinol (At) based on the retention time and a further EAD analysis of a synthetic mixture. In the field trapping experiments, At increased the attraction of I. duplicatus to the traps baited with a binary blend of EM/Id (1:1) in a synergistic manner when tested at a low release rate (1:1:0.1) but had no effect on catches at the high release rate (1:1:1). This result suggests that At might be a part of the Chinese I. duplicatus aggregation pheromone system. The production ratios of EM/Id from the Inner Mongolian population (EM/Id approximate to 1:2.0-4) were twice as high as those reported from Europe (EM/Id approximate to 1:9), corresponding well with the differences in the optimal response ratios.},
  author       = {Zhang, Qing-He and Schlyter, Fredrik and Liu, Guang-Tian and Sheng, Mao-Ling and Birgersson, Göran},
  issn         = {1573-1561},
  keyword      = {scolytidae,enantiomeric composition,Ipsenol,amitinol,attractant,GC-EAD,Ipsdienol,GC-MS,coleoptera,E-myrcenol},
  language     = {eng},
  number       = {7},
  pages        = {1303--1315},
  publisher    = {Springer},
  series       = {Journal of Chemical Ecology},
  title        = {Electrophysiological and behavioral responses of Ips duplicatus to aggregation pheromone in inner mongolia, China: Amitinol as a potential pheromone component},
  url          = {http://dx.doi.org/10.1007/s10886-007-9320-3},
  volume       = {33},
  year         = {2007},
}