L-Arabinose transport and catabolism in yeast
(2007) In The FEBS Journal 274(14). p.3589-3600- Abstract
- Two yeasts, Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012, which show rapid growth on L-arabinose and very high rates of L-arabinose uptake on screening, were selected for characterization of L-arabinose transport and the first steps of intracellular L-arabinose metabolism. The kinetics of L-arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties. The L-arabinose catabolic pathway proposed for filamentous fungi also seems to operate in the yeasts studied. The kinetic parameters of the initial L-arabinose-metabolizing enzymes were determined. Reductases were found to be mostly NADPH-dependent, whereas NAD was the... (More)
- Two yeasts, Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012, which show rapid growth on L-arabinose and very high rates of L-arabinose uptake on screening, were selected for characterization of L-arabinose transport and the first steps of intracellular L-arabinose metabolism. The kinetics of L-arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties. The L-arabinose catabolic pathway proposed for filamentous fungi also seems to operate in the yeasts studied. The kinetic parameters of the initial L-arabinose-metabolizing enzymes were determined. Reductases were found to be mostly NADPH-dependent, whereas NAD was the preferred cofactor of dehydrogenases. The differences found between the two yeasts agree with the higher efficiency of L-arabinose metabolism in C. arabinofermentans. This is the first full account of the initial steps of L-arabinose catabolism in yeast including the biochemical characterization of a specific L-arabinose transporter. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/645786
- author
- Fonseca, Cesar ; Romao, Rute ; de Sousa, Helena Rodrigues ; Hahn-Hägerdal, Bärbel LU and Spencer-Martins, Isabel
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- guilliermondii, Pichia, Candida arabinofermentans, L-arabinose catabolism, sugar transport, yeast
- in
- The FEBS Journal
- volume
- 274
- issue
- 14
- pages
- 3589 - 3600
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000247904900011
- scopus:34447310554
- ISSN
- 1742-464X
- DOI
- 10.1111/j.1742-4658.2007.05892.x
- language
- English
- LU publication?
- yes
- id
- 01c81ea9-730d-4500-9a12-c8d0a209d7ac (old id 645786)
- date added to LUP
- 2016-04-01 16:05:50
- date last changed
- 2022-01-28 17:17:15
@article{01c81ea9-730d-4500-9a12-c8d0a209d7ac, abstract = {{Two yeasts, Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012, which show rapid growth on L-arabinose and very high rates of L-arabinose uptake on screening, were selected for characterization of L-arabinose transport and the first steps of intracellular L-arabinose metabolism. The kinetics of L-arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties. The L-arabinose catabolic pathway proposed for filamentous fungi also seems to operate in the yeasts studied. The kinetic parameters of the initial L-arabinose-metabolizing enzymes were determined. Reductases were found to be mostly NADPH-dependent, whereas NAD was the preferred cofactor of dehydrogenases. The differences found between the two yeasts agree with the higher efficiency of L-arabinose metabolism in C. arabinofermentans. This is the first full account of the initial steps of L-arabinose catabolism in yeast including the biochemical characterization of a specific L-arabinose transporter.}}, author = {{Fonseca, Cesar and Romao, Rute and de Sousa, Helena Rodrigues and Hahn-Hägerdal, Bärbel and Spencer-Martins, Isabel}}, issn = {{1742-464X}}, keywords = {{guilliermondii; Pichia; Candida arabinofermentans; L-arabinose catabolism; sugar transport; yeast}}, language = {{eng}}, number = {{14}}, pages = {{3589--3600}}, publisher = {{Wiley-Blackwell}}, series = {{The FEBS Journal}}, title = {{L-Arabinose transport and catabolism in yeast}}, url = {{http://dx.doi.org/10.1111/j.1742-4658.2007.05892.x}}, doi = {{10.1111/j.1742-4658.2007.05892.x}}, volume = {{274}}, year = {{2007}}, }