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L-Arabinose transport and catabolism in yeast

Fonseca, Cesar; Romao, Rute; de Sousa, Helena Rodrigues; Hahn-Hägerdal, Bärbel LU and Spencer-Martins, Isabel (2007) In The FEBS Journal 274(14). p.3589-3600
Abstract
Two yeasts, Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012, which show rapid growth on L-arabinose and very high rates of L-arabinose uptake on screening, were selected for characterization of L-arabinose transport and the first steps of intracellular L-arabinose metabolism. The kinetics of L-arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties. The L-arabinose catabolic pathway proposed for filamentous fungi also seems to operate in the yeasts studied. The kinetic parameters of the initial L-arabinose-metabolizing enzymes were determined. Reductases were found to be mostly NADPH-dependent, whereas NAD was the... (More)
Two yeasts, Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012, which show rapid growth on L-arabinose and very high rates of L-arabinose uptake on screening, were selected for characterization of L-arabinose transport and the first steps of intracellular L-arabinose metabolism. The kinetics of L-arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties. The L-arabinose catabolic pathway proposed for filamentous fungi also seems to operate in the yeasts studied. The kinetic parameters of the initial L-arabinose-metabolizing enzymes were determined. Reductases were found to be mostly NADPH-dependent, whereas NAD was the preferred cofactor of dehydrogenases. The differences found between the two yeasts agree with the higher efficiency of L-arabinose metabolism in C. arabinofermentans. This is the first full account of the initial steps of L-arabinose catabolism in yeast including the biochemical characterization of a specific L-arabinose transporter. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
guilliermondii, Pichia, Candida arabinofermentans, L-arabinose catabolism, sugar transport, yeast
in
The FEBS Journal
volume
274
issue
14
pages
3589 - 3600
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • wos:000247904900011
  • scopus:34447310554
ISSN
1742-464X
DOI
10.1111/j.1742-4658.2007.05892.x
language
English
LU publication?
yes
id
01c81ea9-730d-4500-9a12-c8d0a209d7ac (old id 645786)
date added to LUP
2007-12-10 13:44:08
date last changed
2017-11-19 04:07:59
@article{01c81ea9-730d-4500-9a12-c8d0a209d7ac,
  abstract     = {Two yeasts, Candida arabinofermentans PYCC 5603(T) and Pichia guilliermondii PYCC 3012, which show rapid growth on L-arabinose and very high rates of L-arabinose uptake on screening, were selected for characterization of L-arabinose transport and the first steps of intracellular L-arabinose metabolism. The kinetics of L-arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties. The L-arabinose catabolic pathway proposed for filamentous fungi also seems to operate in the yeasts studied. The kinetic parameters of the initial L-arabinose-metabolizing enzymes were determined. Reductases were found to be mostly NADPH-dependent, whereas NAD was the preferred cofactor of dehydrogenases. The differences found between the two yeasts agree with the higher efficiency of L-arabinose metabolism in C. arabinofermentans. This is the first full account of the initial steps of L-arabinose catabolism in yeast including the biochemical characterization of a specific L-arabinose transporter.},
  author       = {Fonseca, Cesar and Romao, Rute and de Sousa, Helena Rodrigues and Hahn-Hägerdal, Bärbel and Spencer-Martins, Isabel},
  issn         = {1742-464X},
  keyword      = {guilliermondii,Pichia,Candida arabinofermentans,L-arabinose catabolism,sugar transport,yeast},
  language     = {eng},
  number       = {14},
  pages        = {3589--3600},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {The FEBS Journal},
  title        = {L-Arabinose transport and catabolism in yeast},
  url          = {http://dx.doi.org/10.1111/j.1742-4658.2007.05892.x},
  volume       = {274},
  year         = {2007},
}