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Free flow acoustophoresis: Microfluidic-based mode of particle and cell separation

Petersson, Filip LU ; Aberg, Lena; Sward-Nilsson, Ann-Margret and Laurell, Thomas LU (2007) In Analytical Chemistry 79(14). p.5117-5123
Abstract
A novel method, free flow acoustophoresis (FFA), capable of continuous separation of mixed particle suspensions into multiple outlet fractions is presented. Acoustic forces are utilized to separate particles based on their size and density. The method is shown to be suitable for both biological and nonbiological suspended particles. The microfluidic separation chips were fabricated using conventional microfabrication methods. Particle separation was accomplished by combining laminar flow with the axial acoustic primary radiation force in an ultrasonic standing wave field. Dissimilar suspended particles flowing through the 350-mu m-wide channel were thereby laterally translated to different regions of the laminar flow profile, which was... (More)
A novel method, free flow acoustophoresis (FFA), capable of continuous separation of mixed particle suspensions into multiple outlet fractions is presented. Acoustic forces are utilized to separate particles based on their size and density. The method is shown to be suitable for both biological and nonbiological suspended particles. The microfluidic separation chips were fabricated using conventional microfabrication methods. Particle separation was accomplished by combining laminar flow with the axial acoustic primary radiation force in an ultrasonic standing wave field. Dissimilar suspended particles flowing through the 350-mu m-wide channel were thereby laterally translated to different regions of the laminar flow profile, which was split into multiple outlets for continuous fraction collection. Using four outlets, a mixture of 2-, 5-, 8-, and 10-mu m polystyrene particles was separated with between 62 and 94% of each particle size ending up in separate fractions. Using three outlets and three particle sizes (3, 7, and 10 mu m) the corresponding results ranged between 76 and 96%. It was also proven possible to separate normally acoustically inseparable particle types by manipulating the density of the suspending medium with cesium chloride. The medium manipulation, in combination with FFA, was further used to enable the fractionation of red cells, platelets, and leukocytes. The results show that free flow acoustophoresis can be used to perform complex separation tasks, thereby offering an alternative to expensive and time-consuming methods currently in use. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
79
issue
14
pages
5117 - 5123
publisher
The American Chemical Society
external identifiers
  • wos:000247992600001
  • scopus:34547118407
ISSN
1520-6882
DOI
10.1021/ac070444e
language
English
LU publication?
yes
id
f556d6ad-cfd5-4d8d-9a7f-4ad297d9d55d (old id 646004)
date added to LUP
2007-12-19 13:50:46
date last changed
2017-11-05 03:32:30
@article{f556d6ad-cfd5-4d8d-9a7f-4ad297d9d55d,
  abstract     = {A novel method, free flow acoustophoresis (FFA), capable of continuous separation of mixed particle suspensions into multiple outlet fractions is presented. Acoustic forces are utilized to separate particles based on their size and density. The method is shown to be suitable for both biological and nonbiological suspended particles. The microfluidic separation chips were fabricated using conventional microfabrication methods. Particle separation was accomplished by combining laminar flow with the axial acoustic primary radiation force in an ultrasonic standing wave field. Dissimilar suspended particles flowing through the 350-mu m-wide channel were thereby laterally translated to different regions of the laminar flow profile, which was split into multiple outlets for continuous fraction collection. Using four outlets, a mixture of 2-, 5-, 8-, and 10-mu m polystyrene particles was separated with between 62 and 94% of each particle size ending up in separate fractions. Using three outlets and three particle sizes (3, 7, and 10 mu m) the corresponding results ranged between 76 and 96%. It was also proven possible to separate normally acoustically inseparable particle types by manipulating the density of the suspending medium with cesium chloride. The medium manipulation, in combination with FFA, was further used to enable the fractionation of red cells, platelets, and leukocytes. The results show that free flow acoustophoresis can be used to perform complex separation tasks, thereby offering an alternative to expensive and time-consuming methods currently in use.},
  author       = {Petersson, Filip and Aberg, Lena and Sward-Nilsson, Ann-Margret and Laurell, Thomas},
  issn         = {1520-6882},
  language     = {eng},
  number       = {14},
  pages        = {5117--5123},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Free flow acoustophoresis: Microfluidic-based mode of particle and cell separation},
  url          = {http://dx.doi.org/10.1021/ac070444e},
  volume       = {79},
  year         = {2007},
}