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Ileal pelvic pouch microbiota from two former ulcerative colitis patients, analysed by DNA-based methods, were unstable over time and showed the presence of Clostridium perfringens

Falk, Anders; Olsson, Crister LU ; Ahrné, Siv LU ; Molin, Göran LU ; Adawi, Diya LU and Jeppsson, Bengt LU (2007) In Scandinavian Journal of Gastroenterology 42(8). p.973-985
Abstract
Objective. Ileal pouch anal anastomosis ( IPAA) is the preferred method for restorative surgery in patients with ulcerative colitis who have to undergo proctocolectomy. The most common complication is pouchitis and several studies have pointed to the microbiota of the pouch as being a risk factor. The aim of this study was to follow the development of the bacterial microbiota in pouches during the first year. Material and methods. Terminal restriction fragment length polymorphism ( T-RFLP) combined with cloning and sequencing was used to identify the most predominant bacteria on the different sampling occasions. A total of 274 clones were grouped by T-RFLP and clones from each group were selected for sequencing and identified by comparison... (More)
Objective. Ileal pouch anal anastomosis ( IPAA) is the preferred method for restorative surgery in patients with ulcerative colitis who have to undergo proctocolectomy. The most common complication is pouchitis and several studies have pointed to the microbiota of the pouch as being a risk factor. The aim of this study was to follow the development of the bacterial microbiota in pouches during the first year. Material and methods. Terminal restriction fragment length polymorphism ( T-RFLP) combined with cloning and sequencing was used to identify the most predominant bacteria on the different sampling occasions. A total of 274 clones were grouped by T-RFLP and clones from each group were selected for sequencing and identified by comparison with known sequences. Results. Differences in T-RFLP profiles and clone libraries were found between the patients, and also in changes apparent in each patient at different time-points. The main bacterial groups in the pouches resembled those of the normal colonic microbiota, with a predominance of the clostridia clusters XIVa and IV, Bacteroides and Enterobacteriaceae. Exceptions were clones with sequences resembling those of the Clostridium perfringens group, in both patients and on all sampling occasions, and the dominance of clones resembling Turicibacter in one of the patients at the time of pouch construction. Conclusions. The pouch microbiota showed similarities to the normal colon microbiota except for the presence of clones with sequences resembling those of the C. perfringens group and Turicibacter. The bacterial composition differed between the two patients and the microbiota changed with time, suggesting that the composition is not stable during the first year. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Turicibacter, ileal pouches, terminal restriction fragment, ulcerative colitis, intestinal microbiota, length polymorphism
in
Scandinavian Journal of Gastroenterology
volume
42
issue
8
pages
973 - 985
publisher
Taylor & Francis
external identifiers
  • wos:000247778700012
  • scopus:34447115160
ISSN
1502-7708
DOI
10.1080/00365520701204238
language
English
LU publication?
yes
id
b558c082-312c-41cd-a855-37d31f142483 (old id 646109)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17613928&dopt=Abstract
date added to LUP
2007-12-11 20:31:22
date last changed
2017-06-11 04:29:30
@article{b558c082-312c-41cd-a855-37d31f142483,
  abstract     = {Objective. Ileal pouch anal anastomosis ( IPAA) is the preferred method for restorative surgery in patients with ulcerative colitis who have to undergo proctocolectomy. The most common complication is pouchitis and several studies have pointed to the microbiota of the pouch as being a risk factor. The aim of this study was to follow the development of the bacterial microbiota in pouches during the first year. Material and methods. Terminal restriction fragment length polymorphism ( T-RFLP) combined with cloning and sequencing was used to identify the most predominant bacteria on the different sampling occasions. A total of 274 clones were grouped by T-RFLP and clones from each group were selected for sequencing and identified by comparison with known sequences. Results. Differences in T-RFLP profiles and clone libraries were found between the patients, and also in changes apparent in each patient at different time-points. The main bacterial groups in the pouches resembled those of the normal colonic microbiota, with a predominance of the clostridia clusters XIVa and IV, Bacteroides and Enterobacteriaceae. Exceptions were clones with sequences resembling those of the Clostridium perfringens group, in both patients and on all sampling occasions, and the dominance of clones resembling Turicibacter in one of the patients at the time of pouch construction. Conclusions. The pouch microbiota showed similarities to the normal colon microbiota except for the presence of clones with sequences resembling those of the C. perfringens group and Turicibacter. The bacterial composition differed between the two patients and the microbiota changed with time, suggesting that the composition is not stable during the first year.},
  author       = {Falk, Anders and Olsson, Crister and Ahrné, Siv and Molin, Göran and Adawi, Diya and Jeppsson, Bengt},
  issn         = {1502-7708},
  keyword      = {Turicibacter,ileal pouches,terminal restriction fragment,ulcerative colitis,intestinal microbiota,length polymorphism},
  language     = {eng},
  number       = {8},
  pages        = {973--985},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Gastroenterology},
  title        = {Ileal pelvic pouch microbiota from two former ulcerative colitis patients, analysed by DNA-based methods, were unstable over time and showed the presence of Clostridium perfringens},
  url          = {http://dx.doi.org/10.1080/00365520701204238},
  volume       = {42},
  year         = {2007},
}