Development of polymorphic nuclear microsatellite markers for polyploid and diploid members of the orchid genus Dactylorhiza
(2007) In Molecular Ecology Notes 7(4). p.644-647- Abstract
- Dactylorhiza traunsteineri is an allotetraploid species that belongs to the large Dactylorhiza incarnata/maculata polyploid complex of the Eurasian genus Dactylorhiza (Orchidaceae). Here, eight polymorphic microsatellite loci were isolated using selective hybridization according to the FIASCO protocol (fast isolation by AFLP of sequences containing repeats) with slight modifications. The number of alleles per locus ranged from two to seven. All loci were possible to amplify in several other species of Dactylorhiza, using the same primers.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/646295
- author
- Olofsson, Sofie LU and Hedrén, Mikael LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- narrowed-leaved marsh orchid, microsatellites, cross-species amplification, Dactylorhiza, polymorphism, polyploidy
- in
- Molecular Ecology Notes
- volume
- 7
- issue
- 4
- pages
- 644 - 647
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000247758100028
- scopus:34447114519
- ISSN
- 1471-8278
- DOI
- 10.1111/j.1471-8286.2006.01662.x
- language
- English
- LU publication?
- yes
- id
- 0fbb0aa5-26cb-440a-bece-879b533657b6 (old id 646295)
- date added to LUP
- 2016-04-01 12:16:52
- date last changed
- 2022-02-18 20:22:30
@article{0fbb0aa5-26cb-440a-bece-879b533657b6, abstract = {{Dactylorhiza traunsteineri is an allotetraploid species that belongs to the large Dactylorhiza incarnata/maculata polyploid complex of the Eurasian genus Dactylorhiza (Orchidaceae). Here, eight polymorphic microsatellite loci were isolated using selective hybridization according to the FIASCO protocol (fast isolation by AFLP of sequences containing repeats) with slight modifications. The number of alleles per locus ranged from two to seven. All loci were possible to amplify in several other species of Dactylorhiza, using the same primers.}}, author = {{Olofsson, Sofie and Hedrén, Mikael}}, issn = {{1471-8278}}, keywords = {{narrowed-leaved marsh orchid; microsatellites; cross-species amplification; Dactylorhiza; polymorphism; polyploidy}}, language = {{eng}}, number = {{4}}, pages = {{644--647}}, publisher = {{Wiley-Blackwell}}, series = {{Molecular Ecology Notes}}, title = {{Development of polymorphic nuclear microsatellite markers for polyploid and diploid members of the orchid genus Dactylorhiza}}, url = {{http://dx.doi.org/10.1111/j.1471-8286.2006.01662.x}}, doi = {{10.1111/j.1471-8286.2006.01662.x}}, volume = {{7}}, year = {{2007}}, }