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Development of polymorphic nuclear microsatellite markers for polyploid and diploid members of the orchid genus Dactylorhiza

Olofsson, Sofie LU and Hedrén, Mikael LU (2007) In Molecular Ecology Notes 7(4). p.644-647
Abstract
Dactylorhiza traunsteineri is an allotetraploid species that belongs to the large Dactylorhiza incarnata/maculata polyploid complex of the Eurasian genus Dactylorhiza (Orchidaceae). Here, eight polymorphic microsatellite loci were isolated using selective hybridization according to the FIASCO protocol (fast isolation by AFLP of sequences containing repeats) with slight modifications. The number of alleles per locus ranged from two to seven. All loci were possible to amplify in several other species of Dactylorhiza, using the same primers.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
narrowed-leaved marsh orchid, microsatellites, cross-species amplification, Dactylorhiza, polymorphism, polyploidy
in
Molecular Ecology Notes
volume
7
issue
4
pages
644 - 647
publisher
Wiley-Blackwell
external identifiers
  • wos:000247758100028
  • scopus:34447114519
ISSN
1471-8278
DOI
10.1111/j.1471-8286.2006.01662.x
language
English
LU publication?
yes
id
0fbb0aa5-26cb-440a-bece-879b533657b6 (old id 646295)
date added to LUP
2007-12-14 10:39:08
date last changed
2017-08-13 03:41:51
@article{0fbb0aa5-26cb-440a-bece-879b533657b6,
  abstract     = {Dactylorhiza traunsteineri is an allotetraploid species that belongs to the large Dactylorhiza incarnata/maculata polyploid complex of the Eurasian genus Dactylorhiza (Orchidaceae). Here, eight polymorphic microsatellite loci were isolated using selective hybridization according to the FIASCO protocol (fast isolation by AFLP of sequences containing repeats) with slight modifications. The number of alleles per locus ranged from two to seven. All loci were possible to amplify in several other species of Dactylorhiza, using the same primers.},
  author       = {Olofsson, Sofie and Hedrén, Mikael},
  issn         = {1471-8278},
  keyword      = {narrowed-leaved marsh orchid,microsatellites,cross-species amplification,Dactylorhiza,polymorphism,polyploidy},
  language     = {eng},
  number       = {4},
  pages        = {644--647},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Ecology Notes},
  title        = {Development of polymorphic nuclear microsatellite markers for polyploid and diploid members of the orchid genus Dactylorhiza},
  url          = {http://dx.doi.org/10.1111/j.1471-8286.2006.01662.x},
  volume       = {7},
  year         = {2007},
}