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Cold acclimation and lipid composition in the earthworm Dendrobaena octaedra

Holmstrup, Martin; Sorensen, Louise; Bindesbol, Anne-Mette and Hedlund, Katarina LU (2007) In Comparative Biochemistry and Physiology A 147(4). p.911-919
Abstract
We have investigated the lipid chemistry during cold acclimation in the freeze tolerant earthworm Dendrobaena octaedra. The dominant phospholipid fatty acids (PLFA) of D. octaedra were 20:4, 20:5 and 20:1 (50% of total PLFA) followed by 18:0, 18:1 and 18:2 omega 6,9 (25% of total PLFA). The ability to tolerate freezing in this species was acquired after acclimation at low temperature for 2-4 weeks. During this period one particular membrane PLFA, 18:2 omega 6,9, increased significantly and there was a good correlation between the proportion of this PLFA and the survival of freezing. The composition of neutral lipid fatty acids (NLFA), most likely representing storage lipids (triacylglycerides), also changed during cold acclimation so that... (More)
We have investigated the lipid chemistry during cold acclimation in the freeze tolerant earthworm Dendrobaena octaedra. The dominant phospholipid fatty acids (PLFA) of D. octaedra were 20:4, 20:5 and 20:1 (50% of total PLFA) followed by 18:0, 18:1 and 18:2 omega 6,9 (25% of total PLFA). The ability to tolerate freezing in this species was acquired after acclimation at low temperature for 2-4 weeks. During this period one particular membrane PLFA, 18:2 omega 6,9, increased significantly and there was a good correlation between the proportion of this PLFA and the survival of freezing. The composition of neutral lipid fatty acids (NLFA), most likely representing storage lipids (triacylglycerides), also changed during cold acclimation so that the overall degree of unsaturation increased. Using a common-garden experiment approach, we compared lipid composition of three genetically different populations (Denmark, Finland and Greenland) that differed in their freeze tolerance. Inter-populational differences and differences due to cold acclimation in overall fatty acid composition were evident in both PLFAs and NLFAs. Specifically, the PLFAs, 20:4 and 20:5, were considerably more represented in worms from Greenland, and this contributed to a higher U1 of PLFAs in this population. (c) 2007 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
membrane phospholipids, earthworms, cold acclimation, freeze tolerance, adaptation, homeoviscous
in
Comparative Biochemistry and Physiology A
volume
147
issue
4
pages
911 - 919
publisher
Elsevier
external identifiers
  • wos:000247717000012
  • scopus:34249798163
ISSN
1531-4332
DOI
10.1016/j.cbpa.2007.02.019
language
English
LU publication?
yes
id
418128ab-422c-43c8-8a65-04ccacbe1da2 (old id 647516)
date added to LUP
2007-12-12 15:58:49
date last changed
2017-08-20 03:30:40
@article{418128ab-422c-43c8-8a65-04ccacbe1da2,
  abstract     = {We have investigated the lipid chemistry during cold acclimation in the freeze tolerant earthworm Dendrobaena octaedra. The dominant phospholipid fatty acids (PLFA) of D. octaedra were 20:4, 20:5 and 20:1 (50% of total PLFA) followed by 18:0, 18:1 and 18:2 omega 6,9 (25% of total PLFA). The ability to tolerate freezing in this species was acquired after acclimation at low temperature for 2-4 weeks. During this period one particular membrane PLFA, 18:2 omega 6,9, increased significantly and there was a good correlation between the proportion of this PLFA and the survival of freezing. The composition of neutral lipid fatty acids (NLFA), most likely representing storage lipids (triacylglycerides), also changed during cold acclimation so that the overall degree of unsaturation increased. Using a common-garden experiment approach, we compared lipid composition of three genetically different populations (Denmark, Finland and Greenland) that differed in their freeze tolerance. Inter-populational differences and differences due to cold acclimation in overall fatty acid composition were evident in both PLFAs and NLFAs. Specifically, the PLFAs, 20:4 and 20:5, were considerably more represented in worms from Greenland, and this contributed to a higher U1 of PLFAs in this population. (c) 2007 Elsevier Inc. All rights reserved.},
  author       = {Holmstrup, Martin and Sorensen, Louise and Bindesbol, Anne-Mette and Hedlund, Katarina},
  issn         = {1531-4332},
  keyword      = {membrane phospholipids,earthworms,cold acclimation,freeze tolerance,adaptation,homeoviscous},
  language     = {eng},
  number       = {4},
  pages        = {911--919},
  publisher    = {Elsevier},
  series       = {Comparative Biochemistry and Physiology A},
  title        = {Cold acclimation and lipid composition in the earthworm Dendrobaena octaedra},
  url          = {http://dx.doi.org/10.1016/j.cbpa.2007.02.019},
  volume       = {147},
  year         = {2007},
}