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Poly(acrylamide-allyl glycidyl ether) cryogel as a novel stationary phase in dye-affinity chromatography

Demiryas, Nazan; Tuzmen, Nalan; Galaev, Igor LU ; Piskin, Erhan and Denizli, Adil (2007) In Journal of Applied Polymer Science 105(4). p.1808-1816
Abstract
Poly(acrylamide-allyl glycidyl ether) [poly(AAm-AGE)] cryogel was prepared by bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 mu m size. Cibacron Blue F3GA was immobilized by covalent binding onto poly(AAm-AGE) cryogel via epoxy groups. Poly(AAm-AGE) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly(AAm-AGE) monolithic cryogel was 6.84 g H2O/9 cryogel. Poly(AAm-AGE) cryogel containing 68.9 mu mol Cibacron Blue F3GA/g was used in the adsorption/desorption... (More)
Poly(acrylamide-allyl glycidyl ether) [poly(AAm-AGE)] cryogel was prepared by bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 mu m size. Cibacron Blue F3GA was immobilized by covalent binding onto poly(AAm-AGE) cryogel via epoxy groups. Poly(AAm-AGE) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly(AAm-AGE) monolithic cryogel was 6.84 g H2O/9 cryogel. Poly(AAm-AGE) cryogel containing 68.9 mu mol Cibacron Blue F3GA/g was used in the adsorption/desorption of human serum albumin (HSA) from aqueous solutions and human plasma. The nonspecific adsorption of HSA was very low (0.2 mg/g). The maximum amount of HSA adsorption from aqueous solution in acetate buffer was 27 mg/g at pH 5.0. Higher HSA adsorption value was obtained from human plasma (up to 74.2 mg/g). Desorption of HSA with a purity of 92% from Cibacron Blue F3GA attached poly(AAm-AGE) cryogel was achieved using 0.110 Tris/HCl buffer containing 0.5M NaCl. It was observed that HSA could be repeatedly adsorbed and desorbed with poly(AAm-AGE) cryogel without significant loss in the adsorption capacity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
albumin, protein purification, dye-affinity chromatography, cryogels
in
Journal of Applied Polymer Science
volume
105
issue
4
pages
1808 - 1816
publisher
John Wiley & Sons
external identifiers
  • wos:000247576600012
  • scopus:34547165579
ISSN
1097-4628
DOI
10.1002/app.26187
language
English
LU publication?
yes
id
c266475c-e1b0-403d-a592-e6d9c6441063 (old id 647724)
date added to LUP
2007-12-10 12:29:15
date last changed
2017-10-22 03:32:58
@article{c266475c-e1b0-403d-a592-e6d9c6441063,
  abstract     = {Poly(acrylamide-allyl glycidyl ether) [poly(AAm-AGE)] cryogel was prepared by bulk polymerization which proceeds in an aqueous solution of monomers frozen inside a glass column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 mu m size. Cibacron Blue F3GA was immobilized by covalent binding onto poly(AAm-AGE) cryogel via epoxy groups. Poly(AAm-AGE) cryogel was characterized by swelling studies, FTIR, scanning electron microscopy, and elemental analysis. The equilibrium swelling degree of the poly(AAm-AGE) monolithic cryogel was 6.84 g H2O/9 cryogel. Poly(AAm-AGE) cryogel containing 68.9 mu mol Cibacron Blue F3GA/g was used in the adsorption/desorption of human serum albumin (HSA) from aqueous solutions and human plasma. The nonspecific adsorption of HSA was very low (0.2 mg/g). The maximum amount of HSA adsorption from aqueous solution in acetate buffer was 27 mg/g at pH 5.0. Higher HSA adsorption value was obtained from human plasma (up to 74.2 mg/g). Desorption of HSA with a purity of 92% from Cibacron Blue F3GA attached poly(AAm-AGE) cryogel was achieved using 0.110 Tris/HCl buffer containing 0.5M NaCl. It was observed that HSA could be repeatedly adsorbed and desorbed with poly(AAm-AGE) cryogel without significant loss in the adsorption capacity.},
  author       = {Demiryas, Nazan and Tuzmen, Nalan and Galaev, Igor and Piskin, Erhan and Denizli, Adil},
  issn         = {1097-4628},
  keyword      = {albumin,protein purification,dye-affinity chromatography,cryogels},
  language     = {eng},
  number       = {4},
  pages        = {1808--1816},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Applied Polymer Science},
  title        = {Poly(acrylamide-allyl glycidyl ether) cryogel as a novel stationary phase in dye-affinity chromatography},
  url          = {http://dx.doi.org/10.1002/app.26187},
  volume       = {105},
  year         = {2007},
}