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Continuous full filling capillary electrochromatography: Chromatographic performance and reproducibility

Viberg, Peter LU ; Spégel, Peter LU ; Carlstedt, Jonas LU ; Jornten-Karlsson, Magnus and Petersson, Patrik (2007) In Journal of Chromatography A 1154(1-2). p.386-389
Abstract
Continuous full filling capillary electrochromatography with nanoparticles as pseudostationary phase interfaced with electrospray ionisation mass spectrometric detection was used for reversed phase separations with very high separation efficiency. Several batches of nanoparticles were synthesised and their electrochromatographic performance were evaluated. Different parameters, such as repeatability, reproducibility, limit of detection, and peak asymmetry, were investigated yielding excellent results. The stability of the system over wide pH ranges and over time was found to be excellent. Very high separation efficiencies with over 1.1 million theoretical plates per metre were obtained. The limit of detection for the investigated dialkyl... (More)
Continuous full filling capillary electrochromatography with nanoparticles as pseudostationary phase interfaced with electrospray ionisation mass spectrometric detection was used for reversed phase separations with very high separation efficiency. Several batches of nanoparticles were synthesised and their electrochromatographic performance were evaluated. Different parameters, such as repeatability, reproducibility, limit of detection, and peak asymmetry, were investigated yielding excellent results. The stability of the system over wide pH ranges and over time was found to be excellent. Very high separation efficiencies with over 1.1 million theoretical plates per metre were obtained. The limit of detection for the investigated dialkyl phthalates was approximately 1.0 mu mol L-1, corresponding to 3-5 fmol injected. After preparation, nanoparticle suspensions could be used without further treatment for at least an entire working day with maintained chromatographic qualities. (c) 2007 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
pseudostationary phase, nanoparticles, electrospray ionisation, orthogonal, capillary electrochromatography, mass spectrometry, reversed phase, continuous full filling
in
Journal of Chromatography A
volume
1154
issue
1-2
pages
386 - 389
publisher
Elsevier
external identifiers
  • wos:000247419300045
  • scopus:34249690200
ISSN
0021-9673
DOI
10.1016/j.chroma.2007.02.059
language
English
LU publication?
yes
id
737edb9e-adec-42af-bdad-1c69860473b8 (old id 648058)
date added to LUP
2007-12-20 14:04:19
date last changed
2017-01-01 06:47:42
@article{737edb9e-adec-42af-bdad-1c69860473b8,
  abstract     = {Continuous full filling capillary electrochromatography with nanoparticles as pseudostationary phase interfaced with electrospray ionisation mass spectrometric detection was used for reversed phase separations with very high separation efficiency. Several batches of nanoparticles were synthesised and their electrochromatographic performance were evaluated. Different parameters, such as repeatability, reproducibility, limit of detection, and peak asymmetry, were investigated yielding excellent results. The stability of the system over wide pH ranges and over time was found to be excellent. Very high separation efficiencies with over 1.1 million theoretical plates per metre were obtained. The limit of detection for the investigated dialkyl phthalates was approximately 1.0 mu mol L-1, corresponding to 3-5 fmol injected. After preparation, nanoparticle suspensions could be used without further treatment for at least an entire working day with maintained chromatographic qualities. (c) 2007 Elsevier B.V. All rights reserved.},
  author       = {Viberg, Peter and Spégel, Peter and Carlstedt, Jonas and Jornten-Karlsson, Magnus and Petersson, Patrik},
  issn         = {0021-9673},
  keyword      = {pseudostationary phase,nanoparticles,electrospray ionisation,orthogonal,capillary electrochromatography,mass spectrometry,reversed phase,continuous full filling},
  language     = {eng},
  number       = {1-2},
  pages        = {386--389},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Continuous full filling capillary electrochromatography: Chromatographic performance and reproducibility},
  url          = {http://dx.doi.org/10.1016/j.chroma.2007.02.059},
  volume       = {1154},
  year         = {2007},
}