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Influence of bioreactor scale and complex medium on probing control of glucose feeding in cultivations of recombinant strains of Escherichia coli

Velut, Stephane; Castan, Andreas; Short, Kevin A.; Axelsson, Jan Peter; Hagander, Per LU ; Zditosky, Barry A.; Rysenga, Christopher W.; de Maré, Lena LU and Haglund, Jan (2007) In Biotechnology and Bioengineering 97(4). p.816-824
Abstract
The objective of this work was to evaluate the performance of a feedback glucose control strategy (the probing strategy) in production relevant bioreactors with complex and mineral media. Experimental results from fedbatch cultivations with two recombinant Escherichia coli constructs expressing two different human therapeutic proteins were used to assess the performance and limitations of the glucose probing technique. Even though the performance of the probing strategy was affected by scale and complex media, this methodology rapidly identified a glucose feed protocol similar to an experimentally derived feed regime. This methodology may serve as a powerful tool for industrial process development and in optimization of glucose feed... (More)
The objective of this work was to evaluate the performance of a feedback glucose control strategy (the probing strategy) in production relevant bioreactors with complex and mineral media. Experimental results from fedbatch cultivations with two recombinant Escherichia coli constructs expressing two different human therapeutic proteins were used to assess the performance and limitations of the glucose probing technique. Even though the performance of the probing strategy was affected by scale and complex media, this methodology rapidly identified a glucose feed protocol similar to an experimentally derived feed regime. This methodology may serve as a powerful tool for industrial process development and in optimization of glucose feed regimes when transferring process technology from one bioreactor system to another. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
probing control, feeding strategy, fed-batch, fermentations, industrial, bioreactor, complex medium
in
Biotechnology and Bioengineering
volume
97
issue
4
pages
816 - 824
publisher
John Wiley & Sons
external identifiers
  • wos:000247158800015
  • scopus:34447342536
ISSN
1097-0290
DOI
10.1002/bit.21294
language
English
LU publication?
yes
id
415ba8f8-19b4-490e-aa97-271d4727d44a (old id 648982)
date added to LUP
2008-01-03 11:26:50
date last changed
2017-01-01 04:59:30
@article{415ba8f8-19b4-490e-aa97-271d4727d44a,
  abstract     = {The objective of this work was to evaluate the performance of a feedback glucose control strategy (the probing strategy) in production relevant bioreactors with complex and mineral media. Experimental results from fedbatch cultivations with two recombinant Escherichia coli constructs expressing two different human therapeutic proteins were used to assess the performance and limitations of the glucose probing technique. Even though the performance of the probing strategy was affected by scale and complex media, this methodology rapidly identified a glucose feed protocol similar to an experimentally derived feed regime. This methodology may serve as a powerful tool for industrial process development and in optimization of glucose feed regimes when transferring process technology from one bioreactor system to another.},
  author       = {Velut, Stephane and Castan, Andreas and Short, Kevin A. and Axelsson, Jan Peter and Hagander, Per and Zditosky, Barry A. and Rysenga, Christopher W. and de Maré, Lena and Haglund, Jan},
  issn         = {1097-0290},
  keyword      = {probing control,feeding strategy,fed-batch,fermentations,industrial,bioreactor,complex medium},
  language     = {eng},
  number       = {4},
  pages        = {816--824},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Influence of bioreactor scale and complex medium on probing control of glucose feeding in cultivations of recombinant strains of Escherichia coli},
  url          = {http://dx.doi.org/10.1002/bit.21294},
  volume       = {97},
  year         = {2007},
}