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The γ-tubulin meshwork assists in the recruitment of PCNA to chromatin in mammalian cells

Corvaisier, Matthieu LU ; Zhou, Jingkai LU ; Malycheva, Darina LU ; Cornella, Nicola LU ; Chioureas, Dimitrios ; Gustafsson, Nina ; Rosselló, Catalina Ana ; Ayora, Silvia ; Li, Tongbin and Ekström-Holka, Kristina LU , et al. (2021) In Communications Biology 4.
Abstract
Changes in the location of γ-tubulin ensure cell survival and preserve genome integrity. We investigated whether the nuclear accumulation of γ-tubulin facilitates the transport of proliferating cell nuclear antigen (PCNA) between the cytosolic and the nuclear compartment in mammalian cells. We found that the γ-tubulin meshwork assists in the recruitment of PCNA to chromatin. Also, decreased levels of γ-tubulin reduce the nuclear pool of PCNA. In addition, the γ-tubulin C terminus encodes a PCNA-interacting peptide (PIP) motif, and a γ-tubulin–PIP-mutant affects the nuclear accumulation of PCNA. In a cell-free system, PCNA and γ-tubulin formed a complex. In tumors, there is a significant positive correlation between TUBG1 and PCNA... (More)
Changes in the location of γ-tubulin ensure cell survival and preserve genome integrity. We investigated whether the nuclear accumulation of γ-tubulin facilitates the transport of proliferating cell nuclear antigen (PCNA) between the cytosolic and the nuclear compartment in mammalian cells. We found that the γ-tubulin meshwork assists in the recruitment of PCNA to chromatin. Also, decreased levels of γ-tubulin reduce the nuclear pool of PCNA. In addition, the γ-tubulin C terminus encodes a PCNA-interacting peptide (PIP) motif, and a γ-tubulin–PIP-mutant affects the nuclear accumulation of PCNA. In a cell-free system, PCNA and γ-tubulin formed a complex. In tumors, there is a significant positive correlation between TUBG1 and PCNA expression. Thus, we report a novel mechanism that constitutes the basis for tumor growth by which the γ-tubulin meshwork maintains indefinite proliferation by acting as an opportune scaffold for the transport of PCNA from the cytosol to the chromatin. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Communications Biology
volume
4
article number
767
publisher
Nature Publishing Group
external identifiers
  • scopus:85108581941
  • pmid:34158617
ISSN
2399-3642
DOI
10.1038/s42003-021-02280-1
language
English
LU publication?
yes
id
649b91bd-75c4-4521-94b0-c61baa2ca149
date added to LUP
2021-06-22 11:40:09
date last changed
2024-02-20 08:37:06
@article{649b91bd-75c4-4521-94b0-c61baa2ca149,
  abstract     = {{Changes in the location of γ-tubulin ensure cell survival and preserve genome integrity. We investigated whether the nuclear accumulation of γ-tubulin facilitates the transport of proliferating cell nuclear antigen (PCNA) between the cytosolic and the nuclear compartment in mammalian cells. We found that the γ-tubulin meshwork assists in the recruitment of PCNA to chromatin. Also, decreased levels of γ-tubulin reduce the nuclear pool of PCNA. In addition, the γ-tubulin C terminus encodes a PCNA-interacting peptide (PIP) motif, and a γ-tubulin–PIP-mutant affects the nuclear accumulation of PCNA. In a cell-free system, PCNA and γ-tubulin formed a complex. In tumors, there is a significant positive correlation between TUBG1 and PCNA expression. Thus, we report a novel mechanism that constitutes the basis for tumor growth by which the γ-tubulin meshwork maintains indefinite proliferation by acting as an opportune scaffold for the transport of PCNA from the cytosol to the chromatin.}},
  author       = {{Corvaisier, Matthieu and Zhou, Jingkai and Malycheva, Darina and Cornella, Nicola and Chioureas, Dimitrios and Gustafsson, Nina and Rosselló, Catalina Ana and Ayora, Silvia and Li, Tongbin and Ekström-Holka, Kristina and Jirström, Karin and Lindström, Lisa and Alvarado Kristensson, Maria}},
  issn         = {{2399-3642}},
  language     = {{eng}},
  month        = {{06}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Communications Biology}},
  title        = {{The γ-tubulin meshwork assists in the recruitment of PCNA to chromatin in mammalian cells}},
  url          = {{http://dx.doi.org/10.1038/s42003-021-02280-1}},
  doi          = {{10.1038/s42003-021-02280-1}},
  volume       = {{4}},
  year         = {{2021}},
}