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Solid and fluid segments within the same molecule of stratum corneum ceramide lipid

Pham, Dat LU ; Gooris, Gert; Bouwstra, Joke A; Sparr, Emma LU ; Topgaard, Daniel LU and Mojumdar, Enamul LU (2018) In Quarterly Reviews of Biophysics 51(e7).
Abstract
The outer layer of the skin, stratum corneum (SC) is an efficient transport barrier and it tolerates mechanical deformation. At physiological conditions, the majority of SC lipids are solid, while the presence of a small amount of fluid lipids is considered crucial for SC barrier and material properties. Here we use solid-state and diffusion nuclear magnetic resonance to characterize the composition and molecular dynamics of the fluid lipid fraction in SC model lipids, focusing on the role of the essential SC lipid CER EOS, which is a ceramide esterified omega-hydroxy sphingosine linoleate with very long chain. We show that both rigid and mobile structures are present within the same CER EOS molecule, and that the linoleate segments... (More)
The outer layer of the skin, stratum corneum (SC) is an efficient transport barrier and it tolerates mechanical deformation. At physiological conditions, the majority of SC lipids are solid, while the presence of a small amount of fluid lipids is considered crucial for SC barrier and material properties. Here we use solid-state and diffusion nuclear magnetic resonance to characterize the composition and molecular dynamics of the fluid lipid fraction in SC model lipids, focusing on the role of the essential SC lipid CER EOS, which is a ceramide esterified omega-hydroxy sphingosine linoleate with very long chain. We show that both rigid and mobile structures are present within the same CER EOS molecule, and that the linoleate segments undergo fast isotropic reorientation while exhibiting extraordinarily slow self-diffusion. The characterization of this unusual self-assembly in SC lipids provides deepened insight into the molecular arrangement in the SC extracellular lipid matrix and the role of CER EOS linoleate in the healthy and diseased skin. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Quarterly Reviews of Biophysics
volume
51
issue
e7
pages
9 pages
publisher
Cambridge University Press
external identifiers
  • scopus:85054464022
ISSN
1469-8994
DOI
10.1017/S0033583518000069
language
English
LU publication?
yes
id
649e88df-d23b-402f-b843-00b8cfc3beb0
date added to LUP
2018-11-29 12:07:50
date last changed
2019-02-20 11:38:18
@article{649e88df-d23b-402f-b843-00b8cfc3beb0,
  abstract     = {The outer layer of the skin, stratum corneum (SC) is an efficient transport barrier and it tolerates mechanical deformation. At physiological conditions, the majority of SC lipids are solid, while the presence of a small amount of fluid lipids is considered crucial for SC barrier and material properties. Here we use solid-state and diffusion nuclear magnetic resonance to characterize the composition and molecular dynamics of the fluid lipid fraction in SC model lipids, focusing on the role of the essential SC lipid CER EOS, which is a ceramide esterified omega-hydroxy sphingosine linoleate with very long chain. We show that both rigid and mobile structures are present within the same CER EOS molecule, and that the linoleate segments undergo fast isotropic reorientation while exhibiting extraordinarily slow self-diffusion. The characterization of this unusual self-assembly in SC lipids provides deepened insight into the molecular arrangement in the SC extracellular lipid matrix and the role of CER EOS linoleate in the healthy and diseased skin.},
  author       = {Pham, Dat and Gooris, Gert and Bouwstra, Joke A and Sparr, Emma and Topgaard, Daniel and Mojumdar, Enamul},
  issn         = {1469-8994},
  language     = {eng},
  number       = {e7},
  pages        = {9},
  publisher    = {Cambridge University Press},
  series       = {Quarterly Reviews of Biophysics},
  title        = {Solid and fluid segments within the same molecule of stratum corneum ceramide lipid},
  url          = {http://dx.doi.org/10.1017/S0033583518000069},
  volume       = {51},
  year         = {2018},
}