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The effects of storage and sterilization on de-cellularized and re-cellularized whole lung

Bonenfant, Nicholas R. ; Sokocevic, Dino ; Wagner, Darcy E. LU ; Borg, Zachary D. ; Lathrop, Melissa J. ; Lam, Ying Wai ; Deng, Bin ; DeSarno, Michael J ; Ashikaga, Taka and Loi, Roberto , et al. (2013) In Biomaterials 34(13). p.3231-3245
Abstract

Despite growing interest on the potential use of de-cellularized whole lungs as 3-dimensional scaffolds for ex vivo lung tissue generation, optimal processing including sterilization and storage conditions, are not well defined. Further, it is unclear whether lungs need to be obtained immediately or may be usable even if harvested several days post-mortem, a situation mimicking potential procurement of human lungs from autopsy. We therefore assessed effects of delayed necropsy, prolonged storage (3 and 6 months), and of two commonly utilized sterilization approaches: irradiation or final rinse with peracetic acid, on architecture and extracellular matrix (ECM) protein characteristics of de-cellularized mouse lungs. These different... (More)

Despite growing interest on the potential use of de-cellularized whole lungs as 3-dimensional scaffolds for ex vivo lung tissue generation, optimal processing including sterilization and storage conditions, are not well defined. Further, it is unclear whether lungs need to be obtained immediately or may be usable even if harvested several days post-mortem, a situation mimicking potential procurement of human lungs from autopsy. We therefore assessed effects of delayed necropsy, prolonged storage (3 and 6 months), and of two commonly utilized sterilization approaches: irradiation or final rinse with peracetic acid, on architecture and extracellular matrix (ECM) protein characteristics of de-cellularized mouse lungs. These different approaches resulted in significant differences in both histologic appearance and in retention of ECM and intracellular proteins as assessed by immunohistochemistry and mass spectrometry. Despite these differences, binding and proliferation of bone marrow-derived mesenchymal stromal cells (MSCs) over a one month period following intratracheal inoculation was similar between experimental conditions. In contrast, significant differences occurred with C10 mouse lung epithelial cells between the different conditions. Therefore, delayed necropsy, duration of scaffold storage, sterilization approach, and cell type used for re-cellularization may significantly impact the usefulness of this biological scaffold-based model of ex vivo lung tissue regeneration.

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publishing date
type
Contribution to journal
publication status
published
keywords
Acellular matrix, De-cellularization protocol, Epithelial cell, Extracellular matrix (ECM), Mesenchymal stem cell, Sterilization
in
Biomaterials
volume
34
issue
13
pages
3231 - 3245
publisher
Elsevier
external identifiers
  • scopus:84874231659
ISSN
0142-9612
DOI
10.1016/j.biomaterials.2013.01.031
language
English
LU publication?
no
id
64a14985-9bb5-41f5-957d-1042bceb12a9
date added to LUP
2017-08-15 15:08:40
date last changed
2020-07-01 04:12:14
@article{64a14985-9bb5-41f5-957d-1042bceb12a9,
  abstract     = {<p>Despite growing interest on the potential use of de-cellularized whole lungs as 3-dimensional scaffolds for ex vivo lung tissue generation, optimal processing including sterilization and storage conditions, are not well defined. Further, it is unclear whether lungs need to be obtained immediately or may be usable even if harvested several days post-mortem, a situation mimicking potential procurement of human lungs from autopsy. We therefore assessed effects of delayed necropsy, prolonged storage (3 and 6 months), and of two commonly utilized sterilization approaches: irradiation or final rinse with peracetic acid, on architecture and extracellular matrix (ECM) protein characteristics of de-cellularized mouse lungs. These different approaches resulted in significant differences in both histologic appearance and in retention of ECM and intracellular proteins as assessed by immunohistochemistry and mass spectrometry. Despite these differences, binding and proliferation of bone marrow-derived mesenchymal stromal cells (MSCs) over a one month period following intratracheal inoculation was similar between experimental conditions. In contrast, significant differences occurred with C10 mouse lung epithelial cells between the different conditions. Therefore, delayed necropsy, duration of scaffold storage, sterilization approach, and cell type used for re-cellularization may significantly impact the usefulness of this biological scaffold-based model of ex vivo lung tissue regeneration.</p>},
  author       = {Bonenfant, Nicholas R. and Sokocevic, Dino and Wagner, Darcy E. and Borg, Zachary D. and Lathrop, Melissa J. and Lam, Ying Wai and Deng, Bin and DeSarno, Michael J and Ashikaga, Taka and Loi, Roberto and Weiss, Daniel J.},
  issn         = {0142-9612},
  language     = {eng},
  number       = {13},
  pages        = {3231--3245},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {The effects of storage and sterilization on de-cellularized and re-cellularized whole lung},
  url          = {http://dx.doi.org/10.1016/j.biomaterials.2013.01.031},
  doi          = {10.1016/j.biomaterials.2013.01.031},
  volume       = {34},
  year         = {2013},
}