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Lack of Opsonic Antibody Responses to Invasive Infections With Streptococcus dysgalactiae

Bläckberg, Anna LU ; de Neergaard, Therese LU ; Frick, Inga Maria LU ; Nordenfelt, Pontus LU orcid ; Lood, Rolf LU and Rasmussen, Magnus LU (2021) In Frontiers in Microbiology 12.
Abstract

Introduction: Streptococcus dysgalactiae can cause severe recurrent infections. This study aimed to investigate antibody responses following S. dysgalactiae bacteraemia and possible development of protective immunity. Materials and Methods: Patients with S. dysgalactiae bacteraemia in the county of Skåne between 2017 and 2018 were prospectively included. Acute and convalescent sera were obtained. All isolates were emm typed and enzyme-linked immunosorbent assay (ELISA) was utilised to analyse specific antibody responses to bacteria and antigens. Bactericidal- and phagocytosis assays were applied to further establish antibody function. Results: Sixteen patients with S. dysgalactiae bacteraemia were included of whom one had recurrent... (More)

Introduction: Streptococcus dysgalactiae can cause severe recurrent infections. This study aimed to investigate antibody responses following S. dysgalactiae bacteraemia and possible development of protective immunity. Materials and Methods: Patients with S. dysgalactiae bacteraemia in the county of Skåne between 2017 and 2018 were prospectively included. Acute and convalescent sera were obtained. All isolates were emm typed and enzyme-linked immunosorbent assay (ELISA) was utilised to analyse specific antibody responses to bacteria and antigens. Bactericidal- and phagocytosis assays were applied to further establish antibody function. Results: Sixteen patients with S. dysgalactiae bacteraemia were included of whom one had recurrent episodes of bacteraemia. Using ELISA with S. dysgalactiae isolates and mutants, development of IgG antibodies was demonstrated in few patients. Type-specific antibodies were demonstrated in one patient when recombinant M proteins as antigens, were applied. The type-specific serum mediated a small increase in phagocytosis but did not facilitate increased killing of the S. dysgalactiae isolate, carrying that M protein, in blood or by phagocytic cells. Conclusion: S. dysgalactiae bacteraemia sometimes results in increased levels of antibodies to the infecting pathogen. We did not find evidence that these antibodies are effectively opsonising. Apparent failure to produce opsonising antibodies might partially explain why S. dysgalactiae can cause recurrent invasive infections in the same host.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
antibody responses, bacteraemia, emm type, recurrent infection, Streptococcus dysgalactiae
in
Frontiers in Microbiology
volume
12
article number
635591
publisher
Frontiers Media S. A.
external identifiers
  • pmid:33986732
  • scopus:85105635824
ISSN
1664-302X
DOI
10.3389/fmicb.2021.635591
language
English
LU publication?
yes
additional info
Funding Information: We acknowledge Karl Oldberg, Oonagh Shannon, and Gisela Hovold for important contribution with technical support and help. We also thank the Department of Clinical Microbiology, Office for Medical Services, Region Sk?ne, Lund, Sweden. Funding. This study was funded by the Royal Physiographic Society of Lund, the Thelma Zo?gas Foundation, the Swedish Research Council (VR), the Swedish Government Funds for Clinical Research (ALF), and the foundations of Sk?ne University Hospital, ?sterlund and Lundgren. Funding Information: This study was funded by the Royal Physiographic Society of Lund, the Thelma Zoégas Foundation, the Swedish Research Council (VR), the Swedish Government Funds for Clinical Research (ALF), and the foundations of Skåne University Hospital, Österlund and Lundgren. Publisher Copyright: © Copyright © 2021 Bläckberg, de Neergaard, Frick, Nordenfelt, Lood and Rasmussen. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
id
64e33a48-5aed-4d49-9e74-7f8b42e029f3
date added to LUP
2021-06-09 16:02:04
date last changed
2024-06-15 12:22:49
@article{64e33a48-5aed-4d49-9e74-7f8b42e029f3,
  abstract     = {{<p>Introduction: Streptococcus dysgalactiae can cause severe recurrent infections. This study aimed to investigate antibody responses following S. dysgalactiae bacteraemia and possible development of protective immunity. Materials and Methods: Patients with S. dysgalactiae bacteraemia in the county of Skåne between 2017 and 2018 were prospectively included. Acute and convalescent sera were obtained. All isolates were emm typed and enzyme-linked immunosorbent assay (ELISA) was utilised to analyse specific antibody responses to bacteria and antigens. Bactericidal- and phagocytosis assays were applied to further establish antibody function. Results: Sixteen patients with S. dysgalactiae bacteraemia were included of whom one had recurrent episodes of bacteraemia. Using ELISA with S. dysgalactiae isolates and mutants, development of IgG antibodies was demonstrated in few patients. Type-specific antibodies were demonstrated in one patient when recombinant M proteins as antigens, were applied. The type-specific serum mediated a small increase in phagocytosis but did not facilitate increased killing of the S. dysgalactiae isolate, carrying that M protein, in blood or by phagocytic cells. Conclusion: S. dysgalactiae bacteraemia sometimes results in increased levels of antibodies to the infecting pathogen. We did not find evidence that these antibodies are effectively opsonising. Apparent failure to produce opsonising antibodies might partially explain why S. dysgalactiae can cause recurrent invasive infections in the same host.</p>}},
  author       = {{Bläckberg, Anna and de Neergaard, Therese and Frick, Inga Maria and Nordenfelt, Pontus and Lood, Rolf and Rasmussen, Magnus}},
  issn         = {{1664-302X}},
  keywords     = {{antibody responses; bacteraemia; emm type; recurrent infection; Streptococcus dysgalactiae}},
  language     = {{eng}},
  month        = {{04}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Microbiology}},
  title        = {{Lack of Opsonic Antibody Responses to Invasive Infections With Streptococcus dysgalactiae}},
  url          = {{http://dx.doi.org/10.3389/fmicb.2021.635591}},
  doi          = {{10.3389/fmicb.2021.635591}},
  volume       = {{12}},
  year         = {{2021}},
}