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A novel cell force sensor for quantification of traction during cell spreading and contact guidance

Tymchenko, N.; Wallentin, J.; Petronis, S.; Bjursten, Lars Magnus LU ; Kasemo, B. and Gold, J. (2007) In Biophysical Journal 93(1). p.335-345
Abstract
In this work, we present a ridged, microfabricated, force sensor that can be used to investigate mechanical interactions between cells exhibiting contact guidance and the underlying cell culture substrate, and a proof-of-function evaluation of the force sensor performance. The substrates contain arrays of vertical pillars between solid ridges that were microfabricated in silicon wafers using photolithography and deep reactive ion etching. The spring constant of the pillars was measured by atomic force microscopy. For time-lapse experiments, cells were seeded on the pillared substrates and cultured in an on-stage incubator on a microscope equipped with re. flected differential interference contrast optics. Endothelial cells (ECs) and.... (More)
In this work, we present a ridged, microfabricated, force sensor that can be used to investigate mechanical interactions between cells exhibiting contact guidance and the underlying cell culture substrate, and a proof-of-function evaluation of the force sensor performance. The substrates contain arrays of vertical pillars between solid ridges that were microfabricated in silicon wafers using photolithography and deep reactive ion etching. The spring constant of the pillars was measured by atomic force microscopy. For time-lapse experiments, cells were seeded on the pillared substrates and cultured in an on-stage incubator on a microscope equipped with re. flected differential interference contrast optics. Endothelial cells (ECs) and. broblasts were observed during attachment, spreading, and migration. Custom image analysis software was developed to resolve cell borders, cell alignment to the pillars and migration, displacements of individual pillars, and to quantify cell traction forces. Contact guidance classifi. cation was based on cell alignment and movement angles with respect to microfabricated ridges, as well as cell elongation. In initial investigations made with the ridged cell force sensor, we have observed contact guidance in ECs but not in. broblast cells. A difference in maximal amplitude of mechanical forces was observed between a contact-guided and non-contact-guided, but mobile, EC. However, further experiments are required to determine the statistical significance of this observation. By chance, we observed another feature of cell behavior, namely a reversion of cell force direction. The direction of forces measured under rounded. broblast cells changed from outwards during early cell attachment to inwards during further observation of the spreading phase. The range of forces measured under. broblasts (up to 138 nN) was greater than that measured in EC (up to 57 nN), showing that the rigid silicon sensor is capable of resolving a large range of forces, and hence detection of differences in traction forces between cell types. These observations indicate proof-of-function of the ridged cell force sensor to induce contact guidance, and that the pillared cell force sensor constructed in rigid silicon has the necessary sensitivity to detect differences in traction force vectors between different cell phenotypes and morphologies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biophysical Journal
volume
93
issue
1
pages
335 - 345
publisher
Cell Press
external identifiers
  • wos:000247061500037
  • scopus:34447250752
ISSN
1542-0086
DOI
10.1529/biophysj.106.093302
language
English
LU publication?
yes
id
2740cd8f-d8f8-4658-8c7f-5536029a3329 (old id 651154)
date added to LUP
2007-12-19 11:00:55
date last changed
2017-11-19 03:31:19
@article{2740cd8f-d8f8-4658-8c7f-5536029a3329,
  abstract     = {In this work, we present a ridged, microfabricated, force sensor that can be used to investigate mechanical interactions between cells exhibiting contact guidance and the underlying cell culture substrate, and a proof-of-function evaluation of the force sensor performance. The substrates contain arrays of vertical pillars between solid ridges that were microfabricated in silicon wafers using photolithography and deep reactive ion etching. The spring constant of the pillars was measured by atomic force microscopy. For time-lapse experiments, cells were seeded on the pillared substrates and cultured in an on-stage incubator on a microscope equipped with re. flected differential interference contrast optics. Endothelial cells (ECs) and. broblasts were observed during attachment, spreading, and migration. Custom image analysis software was developed to resolve cell borders, cell alignment to the pillars and migration, displacements of individual pillars, and to quantify cell traction forces. Contact guidance classifi. cation was based on cell alignment and movement angles with respect to microfabricated ridges, as well as cell elongation. In initial investigations made with the ridged cell force sensor, we have observed contact guidance in ECs but not in. broblast cells. A difference in maximal amplitude of mechanical forces was observed between a contact-guided and non-contact-guided, but mobile, EC. However, further experiments are required to determine the statistical significance of this observation. By chance, we observed another feature of cell behavior, namely a reversion of cell force direction. The direction of forces measured under rounded. broblast cells changed from outwards during early cell attachment to inwards during further observation of the spreading phase. The range of forces measured under. broblasts (up to 138 nN) was greater than that measured in EC (up to 57 nN), showing that the rigid silicon sensor is capable of resolving a large range of forces, and hence detection of differences in traction forces between cell types. These observations indicate proof-of-function of the ridged cell force sensor to induce contact guidance, and that the pillared cell force sensor constructed in rigid silicon has the necessary sensitivity to detect differences in traction force vectors between different cell phenotypes and morphologies.},
  author       = {Tymchenko, N. and Wallentin, J. and Petronis, S. and Bjursten, Lars Magnus and Kasemo, B. and Gold, J.},
  issn         = {1542-0086},
  language     = {eng},
  number       = {1},
  pages        = {335--345},
  publisher    = {Cell Press},
  series       = {Biophysical Journal},
  title        = {A novel cell force sensor for quantification of traction during cell spreading and contact guidance},
  url          = {http://dx.doi.org/10.1529/biophysj.106.093302},
  volume       = {93},
  year         = {2007},
}