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Transition of the ability to generate petites in the Saccharomyces/Kluyveromyces complex

Fekete, Veronika; Cierna, Maria; Poláková, Silvia LU ; Piskur, Jure LU and Sulo, Pavol (2007) In FEMS Yeast Research 7(8). p.1237-1247
Abstract
Petite-positivity - the ability to tolerate the loss of mtDNA - was examined after the treatment with ethidium bromide (EB) in over hundred isolates from the Saccharomyces/Kluyveromyces complex. The identity of petite mutants was confirmed by the loss of specific mtDNA DAPI staining patterns. Besides unequivocal petite-positive and petite-negative phenotypes, a few species exhibited temperature sensitive petite positive phenotype and petiteness of a few other species could be observed only at the elevated EB concentrations. Several yeast species displayed a mixed 'moot' phenotype, where a major part of the population did not tolerate the loss of mtDNA but several cells did. The genera from postwhole-genome duplication lineages... (More)
Petite-positivity - the ability to tolerate the loss of mtDNA - was examined after the treatment with ethidium bromide (EB) in over hundred isolates from the Saccharomyces/Kluyveromyces complex. The identity of petite mutants was confirmed by the loss of specific mtDNA DAPI staining patterns. Besides unequivocal petite-positive and petite-negative phenotypes, a few species exhibited temperature sensitive petite positive phenotype and petiteness of a few other species could be observed only at the elevated EB concentrations. Several yeast species displayed a mixed 'moot' phenotype, where a major part of the population did not tolerate the loss of mtDNA but several cells did. The genera from postwhole-genome duplication lineages (Saccharomyces, Kazachstania, Naumovia, Nakaseomyces) were invariably petite-positive. However, petite-positive traits could also be observed among the prewhole-genome duplication species. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ethidium, Saccharomyces/Kluyveromyces complex, petite mutation, mtDNA, bromide, mitochondria
in
FEMS Yeast Research
volume
7
issue
8
pages
1237 - 1247
publisher
Elsevier
external identifiers
  • wos:000250298000004
  • scopus:35448951798
ISSN
1567-1364
DOI
10.1111/j.1567-1364.2007.00287.x
language
English
LU publication?
yes
id
fa59bda7-43f9-49a0-a935-4edbf760fd95 (old id 653143)
date added to LUP
2007-12-11 20:46:46
date last changed
2017-01-01 04:37:49
@article{fa59bda7-43f9-49a0-a935-4edbf760fd95,
  abstract     = {Petite-positivity - the ability to tolerate the loss of mtDNA - was examined after the treatment with ethidium bromide (EB) in over hundred isolates from the Saccharomyces/Kluyveromyces complex. The identity of petite mutants was confirmed by the loss of specific mtDNA DAPI staining patterns. Besides unequivocal petite-positive and petite-negative phenotypes, a few species exhibited temperature sensitive petite positive phenotype and petiteness of a few other species could be observed only at the elevated EB concentrations. Several yeast species displayed a mixed 'moot' phenotype, where a major part of the population did not tolerate the loss of mtDNA but several cells did. The genera from postwhole-genome duplication lineages (Saccharomyces, Kazachstania, Naumovia, Nakaseomyces) were invariably petite-positive. However, petite-positive traits could also be observed among the prewhole-genome duplication species.},
  author       = {Fekete, Veronika and Cierna, Maria and Poláková, Silvia and Piskur, Jure and Sulo, Pavol},
  issn         = {1567-1364},
  keyword      = {ethidium,Saccharomyces/Kluyveromyces complex,petite mutation,mtDNA,bromide,mitochondria},
  language     = {eng},
  number       = {8},
  pages        = {1237--1247},
  publisher    = {Elsevier},
  series       = {FEMS Yeast Research},
  title        = {Transition of the ability to generate petites in the Saccharomyces/Kluyveromyces complex},
  url          = {http://dx.doi.org/10.1111/j.1567-1364.2007.00287.x},
  volume       = {7},
  year         = {2007},
}