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Genetic variation in the major mitotic checkpoint genes associated with chromosomal aberrations in healthy humans

Försti, Asta LU ; Frank, Christoph LU ; Smolkova, Bozena; Kazimirova, Alena; Barancokova, Magdalena; Vymetalkova, Veronika; Kroupa, Michal; Naccarati, Alessio; Vodickova, Ludmila and Buchancova, Janka, et al. (2016) In Cancer Letters 380(2). p.442-446
Abstract

Non-specific chromosomal aberrations (CAs) are microscopically detected in about 1% of lymphocytes drawn from healthy persons. Causes of CAs in general population are not known but they may be related to risk of cancer. In view of the importance of the mitotic checkpoint machinery on maintaining chromosomal integrity we selected 9 variants in main checkpoint related genes (BUB1B, BUB3, MAD2L1, CENPF, ESPL1/separase, NEK2, PTTG1/securin, ZWILCH and ZWINT) for a genotyping study on samples from healthy individuals (N = 330 to 729) whose lymphocytes had an increased number of CAs compared to persons with a low number of CAs. Genetic variation in individual genes played a minor importance, consistent with the high conservation and selection... (More)

Non-specific chromosomal aberrations (CAs) are microscopically detected in about 1% of lymphocytes drawn from healthy persons. Causes of CAs in general population are not known but they may be related to risk of cancer. In view of the importance of the mitotic checkpoint machinery on maintaining chromosomal integrity we selected 9 variants in main checkpoint related genes (BUB1B, BUB3, MAD2L1, CENPF, ESPL1/separase, NEK2, PTTG1/securin, ZWILCH and ZWINT) for a genotyping study on samples from healthy individuals (N = 330 to 729) whose lymphocytes had an increased number of CAs compared to persons with a low number of CAs. Genetic variation in individual genes played a minor importance, consistent with the high conservation and selection pressure of the checkpoint system. However, gene pairs were significantly associated with CAs: PTTG1-ZWILCH and PTTG1-ZWINT. MAD2L1 and PTTG1 were the most common partners in any of the two-way interactions. The results suggest that interactions at the level of cohesin (PTTG1) and kinetochore function (ZWINT, ZWILCH and MAD2L1) contribute to the frequency of CAs, suggesting that gene variants at different checkpoint functions appeared to be required for the formation of CAs.

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publication status
published
subject
keywords
Chromosomal integrity, Cytogenetics, DNA double-stranded break, Spindle checkpoint
in
Cancer Letters
volume
380
issue
2
pages
5 pages
publisher
Elsevier
external identifiers
  • scopus:84978639705
  • wos:000383300300009
ISSN
0304-3835
DOI
10.1016/j.canlet.2016.07.011
language
English
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yes
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653a80f1-1dac-44f6-bc07-6bea334b7e21
date added to LUP
2016-08-01 10:59:51
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2017-04-11 16:22:44
@article{653a80f1-1dac-44f6-bc07-6bea334b7e21,
  abstract     = {<p>Non-specific chromosomal aberrations (CAs) are microscopically detected in about 1% of lymphocytes drawn from healthy persons. Causes of CAs in general population are not known but they may be related to risk of cancer. In view of the importance of the mitotic checkpoint machinery on maintaining chromosomal integrity we selected 9 variants in main checkpoint related genes (BUB1B, BUB3, MAD2L1, CENPF, ESPL1/separase, NEK2, PTTG1/securin, ZWILCH and ZWINT) for a genotyping study on samples from healthy individuals (N = 330 to 729) whose lymphocytes had an increased number of CAs compared to persons with a low number of CAs. Genetic variation in individual genes played a minor importance, consistent with the high conservation and selection pressure of the checkpoint system. However, gene pairs were significantly associated with CAs: PTTG1-ZWILCH and PTTG1-ZWINT. MAD2L1 and PTTG1 were the most common partners in any of the two-way interactions. The results suggest that interactions at the level of cohesin (PTTG1) and kinetochore function (ZWINT, ZWILCH and MAD2L1) contribute to the frequency of CAs, suggesting that gene variants at different checkpoint functions appeared to be required for the formation of CAs.</p>},
  author       = {Försti, Asta and Frank, Christoph and Smolkova, Bozena and Kazimirova, Alena and Barancokova, Magdalena and Vymetalkova, Veronika and Kroupa, Michal and Naccarati, Alessio and Vodickova, Ludmila and Buchancova, Janka and Dusinska, Maria and Musak, Ludovit and Vodicka, Pavel and Hemminki, Kari},
  issn         = {0304-3835},
  keyword      = {Chromosomal integrity,Cytogenetics,DNA double-stranded break,Spindle checkpoint},
  language     = {eng},
  month        = {10},
  number       = {2},
  pages        = {442--446},
  publisher    = {Elsevier},
  series       = {Cancer Letters},
  title        = {Genetic variation in the major mitotic checkpoint genes associated with chromosomal aberrations in healthy humans},
  url          = {http://dx.doi.org/10.1016/j.canlet.2016.07.011},
  volume       = {380},
  year         = {2016},
}