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High-throughput proteomics using antibody microarrays: an update

Borrebaeck, Carl LU and Wingren, Christer LU (2007) In Expert Review of Molecular Diagnostics 7(5). p.673-686
Abstract
Antibody-based microarrays are a rapidly emerging technology that has advanced from the first proof-of-concept studies to demanding serum protein profiling applications during recent years, displaying great promise within disease proteomics. Miniaturized micro- and nanoarrays can be fabricated with an almost infinite number of antibodies carrying the desired specificities. While consuming only minute amounts of reagents, multiplexed and ultrasensitive assays can be performed targeting high- as well as low-abundance analytes in complex nonfractionated proteomes. The microarray images generated can then be converted into protein expression profiles or protein atlases, revealing a detailed composition of the sample. The technology will... (More)
Antibody-based microarrays are a rapidly emerging technology that has advanced from the first proof-of-concept studies to demanding serum protein profiling applications during recent years, displaying great promise within disease proteomics. Miniaturized micro- and nanoarrays can be fabricated with an almost infinite number of antibodies carrying the desired specificities. While consuming only minute amounts of reagents, multiplexed and ultrasensitive assays can be performed targeting high- as well as low-abundance analytes in complex nonfractionated proteomes. The microarray images generated can then be converted into protein expression profiles or protein atlases, revealing a detailed composition of the sample. The technology will provide unique opportunities for fields such as disease diagnostics, biomarker discovery, patient stratification, predicting disease recurrence and drug target discovery. This review describes an update of high-throughput proteomics, using antibody-based microarrays, focusing on key technological advances and novel applications that have emerged over the last 3 years. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
recombinant, proteomics, protein array, oncoproteomics, nanoarrays, disease proteomics, affinity proteomics, antibody microarrays, antibody microarray
in
Expert Review of Molecular Diagnostics
volume
7
issue
5
pages
673 - 686
publisher
Future Drugs Ltd
external identifiers
  • wos:000250321400020
  • scopus:35148842343
ISSN
1744-8352
DOI
10.1586/14737159.7.5.673
project
CREATE Health
language
English
LU publication?
yes
id
c181473e-8bce-47c9-8865-54ff7fed6cb8 (old id 654052)
date added to LUP
2007-12-11 15:31:26
date last changed
2017-09-03 03:58:14
@article{c181473e-8bce-47c9-8865-54ff7fed6cb8,
  abstract     = {Antibody-based microarrays are a rapidly emerging technology that has advanced from the first proof-of-concept studies to demanding serum protein profiling applications during recent years, displaying great promise within disease proteomics. Miniaturized micro- and nanoarrays can be fabricated with an almost infinite number of antibodies carrying the desired specificities. While consuming only minute amounts of reagents, multiplexed and ultrasensitive assays can be performed targeting high- as well as low-abundance analytes in complex nonfractionated proteomes. The microarray images generated can then be converted into protein expression profiles or protein atlases, revealing a detailed composition of the sample. The technology will provide unique opportunities for fields such as disease diagnostics, biomarker discovery, patient stratification, predicting disease recurrence and drug target discovery. This review describes an update of high-throughput proteomics, using antibody-based microarrays, focusing on key technological advances and novel applications that have emerged over the last 3 years.},
  author       = {Borrebaeck, Carl and Wingren, Christer},
  issn         = {1744-8352},
  keyword      = {recombinant,proteomics,protein array,oncoproteomics,nanoarrays,disease proteomics,affinity proteomics,antibody microarrays,antibody microarray},
  language     = {eng},
  number       = {5},
  pages        = {673--686},
  publisher    = {Future Drugs Ltd},
  series       = {Expert Review of Molecular Diagnostics},
  title        = {High-throughput proteomics using antibody microarrays: an update},
  url          = {http://dx.doi.org/10.1586/14737159.7.5.673},
  volume       = {7},
  year         = {2007},
}