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On the importance of the support material for bioorganic synthesis : Influence of water partition between solvent, enzyme and solid support in water‐poor reaction media

RESLOW, Mats ; ADLERCREUTZ, Patrick LU orcid and MATTIASSON, Bo LU (1988) In European Journal of Biochemistry 172(3). p.573-578
Abstract

α‐Chymotrypsin was adsorbed on solid support materials and the catalytic activity of the preparations in organic solvents was studied. The activity was highly dependent on the nature of the support material and on the amount of water present in the reaction mixture. There appears to be competition for the water in the system between the enzyme, the support material and the solvent. The support materials were characterized by measuring their ability to absorb water from water‐saturated diisopropyl ether. For the quotient: (amount of water on the support)/(amount of water in the solvent) in the model system the term aquaphilicity was proposed. The activity of adsorbed chymotrypsin in diisopropyl ether decreased with increasing... (More)

α‐Chymotrypsin was adsorbed on solid support materials and the catalytic activity of the preparations in organic solvents was studied. The activity was highly dependent on the nature of the support material and on the amount of water present in the reaction mixture. There appears to be competition for the water in the system between the enzyme, the support material and the solvent. The support materials were characterized by measuring their ability to absorb water from water‐saturated diisopropyl ether. For the quotient: (amount of water on the support)/(amount of water in the solvent) in the model system the term aquaphilicity was proposed. The activity of adsorbed chymotrypsin in diisopropyl ether decreased with increasing aquaphilicity of the support material. The same trend was observed when the activity of horse liver alcohol dehydrogenase adsorbed on different supports was measured in diisopropyl ether.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Biochemistry
volume
172
issue
3
pages
6 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0024287153
  • pmid:3350014
ISSN
0014-2956
DOI
10.1111/j.1432-1033.1988.tb13927.x
language
English
LU publication?
yes
id
6550aa6b-aadd-4a36-b738-0acdfd1ff868
date added to LUP
2019-06-22 18:58:36
date last changed
2024-01-01 12:28:44
@article{6550aa6b-aadd-4a36-b738-0acdfd1ff868,
  abstract     = {{<p>α‐Chymotrypsin was adsorbed on solid support materials and the catalytic activity of the preparations in organic solvents was studied. The activity was highly dependent on the nature of the support material and on the amount of water present in the reaction mixture. There appears to be competition for the water in the system between the enzyme, the support material and the solvent. The support materials were characterized by measuring their ability to absorb water from water‐saturated diisopropyl ether. For the quotient: (amount of water on the support)/(amount of water in the solvent) in the model system the term aquaphilicity was proposed. The activity of adsorbed chymotrypsin in diisopropyl ether decreased with increasing aquaphilicity of the support material. The same trend was observed when the activity of horse liver alcohol dehydrogenase adsorbed on different supports was measured in diisopropyl ether.</p>}},
  author       = {{RESLOW, Mats and ADLERCREUTZ, Patrick and MATTIASSON, Bo}},
  issn         = {{0014-2956}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{3}},
  pages        = {{573--578}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{European Journal of Biochemistry}},
  title        = {{On the importance of the support material for bioorganic synthesis : Influence of water partition between solvent, enzyme and solid support in water‐poor reaction media}},
  url          = {{http://dx.doi.org/10.1111/j.1432-1033.1988.tb13927.x}},
  doi          = {{10.1111/j.1432-1033.1988.tb13927.x}},
  volume       = {{172}},
  year         = {{1988}},
}