On the importance of the support material for bioorganic synthesis : Influence of water partition between solvent, enzyme and solid support in water‐poor reaction media
(1988) In European Journal of Biochemistry 172(3). p.573-578- Abstract
α‐Chymotrypsin was adsorbed on solid support materials and the catalytic activity of the preparations in organic solvents was studied. The activity was highly dependent on the nature of the support material and on the amount of water present in the reaction mixture. There appears to be competition for the water in the system between the enzyme, the support material and the solvent. The support materials were characterized by measuring their ability to absorb water from water‐saturated diisopropyl ether. For the quotient: (amount of water on the support)/(amount of water in the solvent) in the model system the term aquaphilicity was proposed. The activity of adsorbed chymotrypsin in diisopropyl ether decreased with increasing... (More)
α‐Chymotrypsin was adsorbed on solid support materials and the catalytic activity of the preparations in organic solvents was studied. The activity was highly dependent on the nature of the support material and on the amount of water present in the reaction mixture. There appears to be competition for the water in the system between the enzyme, the support material and the solvent. The support materials were characterized by measuring their ability to absorb water from water‐saturated diisopropyl ether. For the quotient: (amount of water on the support)/(amount of water in the solvent) in the model system the term aquaphilicity was proposed. The activity of adsorbed chymotrypsin in diisopropyl ether decreased with increasing aquaphilicity of the support material. The same trend was observed when the activity of horse liver alcohol dehydrogenase adsorbed on different supports was measured in diisopropyl ether.
(Less)
- author
- RESLOW, Mats ; ADLERCREUTZ, Patrick LU and MATTIASSON, Bo LU
- organization
- publishing date
- 1988-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Biochemistry
- volume
- 172
- issue
- 3
- pages
- 6 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:3350014
- scopus:0024287153
- ISSN
- 0014-2956
- DOI
- 10.1111/j.1432-1033.1988.tb13927.x
- language
- English
- LU publication?
- yes
- id
- 6550aa6b-aadd-4a36-b738-0acdfd1ff868
- date added to LUP
- 2019-06-22 18:58:36
- date last changed
- 2024-01-01 12:28:44
@article{6550aa6b-aadd-4a36-b738-0acdfd1ff868, abstract = {{<p>α‐Chymotrypsin was adsorbed on solid support materials and the catalytic activity of the preparations in organic solvents was studied. The activity was highly dependent on the nature of the support material and on the amount of water present in the reaction mixture. There appears to be competition for the water in the system between the enzyme, the support material and the solvent. The support materials were characterized by measuring their ability to absorb water from water‐saturated diisopropyl ether. For the quotient: (amount of water on the support)/(amount of water in the solvent) in the model system the term aquaphilicity was proposed. The activity of adsorbed chymotrypsin in diisopropyl ether decreased with increasing aquaphilicity of the support material. The same trend was observed when the activity of horse liver alcohol dehydrogenase adsorbed on different supports was measured in diisopropyl ether.</p>}}, author = {{RESLOW, Mats and ADLERCREUTZ, Patrick and MATTIASSON, Bo}}, issn = {{0014-2956}}, language = {{eng}}, month = {{01}}, number = {{3}}, pages = {{573--578}}, publisher = {{Wiley-Blackwell}}, series = {{European Journal of Biochemistry}}, title = {{On the importance of the support material for bioorganic synthesis : Influence of water partition between solvent, enzyme and solid support in water‐poor reaction media}}, url = {{http://dx.doi.org/10.1111/j.1432-1033.1988.tb13927.x}}, doi = {{10.1111/j.1432-1033.1988.tb13927.x}}, volume = {{172}}, year = {{1988}}, }